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dc.contributor.authorVera-Gonzales, Corinaes_ES
dc.contributor.authorArenas-Chávez, Carlos Albertoes_ES
dc.contributor.authorPonce-Soto, Luis A.es_ES
dc.contributor.authorAlvarez-Risco, Aldoes_ES
dc.contributor.authorDel-Aguila-Arcentales, Shylaes_ES
dc.contributor.authorDavies, Neal M.es_ES
dc.contributor.authorYáñez, Jaime A.es_ES
dc.date.accessioned2022-11-18T15:58:08Z
dc.date.available2022-11-18T15:58:08Z
dc.date.issued2022-07-21
dc.identifier.urihttps://hdl.handle.net/20.500.13053/7148
dc.description.abstractThe fractionation of Crotalus durissus collilineatus whole venom through an HPLC chromatographic method enabled the purification of a new V-1 neurotoxin. Inter-CRO (V-1) presents similarity in its primary structure to crotoxin B (CB), suggesting another isoform of this toxin. The aim of this study was to compare V-1 to the crotoxin complex (CA/CB) and CB to elucidate aspects related to its functionality. The homogeneity of the purified protein was confirmed with a molecular mass of 1425.45 Da, further verified by mass spectrometry. The sequence of the protein showed high similarity to other viperid snake venom PLA2 proteins. The results of this study report that V-1 is an uncharacterized novel toxin with different biological activities from CB. V-1 maintained catalytic activity but presented neurotoxic activity as observed by the 2.5-fold increase in twitch tension record compared to control values on isolated muscle cells. View Full-Textes_ES
dc.formatapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/es_ES
dc.subjectCrotalus durissus collilineatus; Crotalus durissus terrificus; neurotoxin; V-1; HPLC-RPes_ES
dc.titlePurification and Characterization of a Novel Factor of Crotoxin Inter-CRO (V-1), a New Phospholipase A2 Isoform from Crotalus durissus collilineatus Snake Venom Using an In Vitro Neuromuscular Preparationes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doihttps://doi.org/10.3390/pr10071428es_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dc.publisher.countryGBes_ES
dc.subject.ocdehttp://purl.org/pe-repo/ocde/ford#3.03.00es_ES


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