Millones Gómez, Pablo AlejandroRequena Mendizábal, Margarita FeCalla Poma, Roger DamasoRosales Cifuentes, Tania ValentinaMalpartida Quispe, Federico MartinMaurtua Torres, Dora JesúsBacilio Amaranto, Reyma EvelynMinchón Medina, Carlos AlbertoPonce Contreras, Lusin Antonio2022-10-252022-10-252022https://hdl.handle.net/20.500.13053/6926Objective: To assess the antibacterial activity of Psidium guajava fractions and their effects on adhesion of a multispecies biofilm consisting of Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis in vitro. Material and Methods: Guava leaves were obtained from the mountains of northern Peru, where they grow wild and free of pesticides. The antimicrobial activity of 25 mg/mL petroleum ether, 25 mg/mL dichloromethane and 25 mg/mL methanol fractions of P. guajava was evaluated by measuring inhibition halos, as well as the effect on the adhesion of multispecies biofilms at 4, 7 and 10 days of growth by measuring the optical density. In addition, antimicrobial susceptibility was compared using the Kruskal-Wallis test and its multiple comparison tests, and differences in mean biofilm adhesion between each fraction were assessed by repeated measures analysis and the Tukey multiple comparison test. Results: The rank-based Kruskal-Wallis test highlighted differences in the effects of the fractions on the zone of inhibition for each oral bacterium, including S. gordonii(p=0.000), F. nucleatum (p=0.000), and P. gingivalis (p=0.000), the Tukey test showed that the group treated with 0.12% chlorhexidine exhibited the least amount of adhesion, followed by the group treated with the 1.56 mg/mL methanol fraction. Conclusion: The methanol fraction of P. guajava had an antibacterial effect on S. gordonii and P. gingivalis, and the 1.56 mg/mL methanol fraction decreased biofilm adhesion.application/pdfenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/4.0/Psidium, Biofilms, Gram-Negative Bacteriainfo:eu-repo/semantics/articlehttps://doi.org/10.1590/pboci.2022.028ISSN 1519-0501 / eISSN 1983-4632http://purl.org/pe-repo/ocde/ford#3.03.00