dc.contributor.author | Rosales-Rimache, Jaime | es_ES |
dc.contributor.author | Nunayalle-Vargas, Magda | es_ES |
dc.contributor.author | Rueda-Torres, Lenin | es_ES |
dc.contributor.author | Inolopú-Cucche, Jorge | es_ES |
dc.date.accessioned | 2023-03-14T20:27:11Z | |
dc.date.available | 2023-03-14T20:27:11Z | |
dc.date.issued | 2022-12-22 | |
dc.identifier.uri | https://hdl.handle.net/20.500.13053/8099 | |
dc.description.abstract | Background: Sputum smear microscopy (SSM) is a screening test used to diagnose tuberculosis (TB); however, its performance and sensitivity are relatively low, which can lead to false negatives. We designed a cross-sectional study to estimate the performance of SSM that includes a pretreatment based on sputum digestion with bleach (sodium hypochlorite) for the diagnosis of TB. Methods: We evaluated 73 sputum samples from patients with a diagnosis of TB confirmed by the Xpert MTB/RIF test and 114 samples from patients without TB. We performed sputum digestion using a 5% sodium hypochlorite solution, centrifuged at 2000 rpm for 15 min. We prepared smears for direct and bleach-treated SSM and used Ziehl–Neelsen staining. Results: The bleach-treated SSM obtained absolute identification of the cases of TB confirmed by the Xpert test, compared to 95.9% identified by the direct smear method (without bleach treatment). We also found a significant increase (p < 0.001) in the recovery of acid-fast bacilli (AFB) obtained by the bleach-treated SSM (293.8 ± 215.1 AFB) compared to the direct SSM method (222.9 ± 195.5 AFB). The AUC of the bleach-treated SSM and direct SSM was 100% and 96.6%, respectively. Conclusion: The bleach-treated SSM performs better than the direct SSM in identifying AFB and increasing the bacillary count in the sputum samples. | es_ES |
dc.format | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.subject | tuberculosis; bacilloscopy; sputum | es_ES |
dc.title | Performance of Bleach Method Sputum Smear Microscopy for the Diagnosis of Tuberculosis in a Highly Endemic District in Lima, Peru | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | https://doi.org/10.3390/ijerph20010135 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.publisher.country | CH | es_ES |
dc.subject.ocde | http://purl.org/pe-repo/ocde/ford#3.03.00 | es_ES |