Examinando por Autor "Aliaga Pastor, Carlos Mario"

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    Efecto hepatoprotector del extracto etanólico del tegumento de Raphanus sativus L. “rabanito” sobre la hepatotoxicidad inducida por paracetamol en ratas Holtzman - Lima, 2024
    (Universidad Privada Norbert Wiener, 2024-10-09) Aliaga Pastor, Carlos Mario; Gutiérrez Cachique, Cinthia Pierina; Ñañez del Pino, Daniel
    Objective: Determine the hepatoprotective effect of the ethanolic extract of the integument of Raphanus sativus L. “rabanito” on paracetamol-induced hepatotoxicity in Holtzman rats. Materials and methods: Solubility tests and a phytochemical study were carried out on the ethanolic extract of the integument of Raphanus sativus L., and the hepatoprotective effect on paracetamol-induced intoxication compared to silymarin was determined. An experimental, quantitative and transversal design was applied, for this, 48 Holtzman rats were used, which were distributed into 6 groups of 8 rats each, different treatments were administered: White Group; Negative Control Group (Physiological saline + paracetamol); Positive Control Group: (Silymarin + paracetamol), Experimental group 1: (100mg/kg + 400mg/kg paracetamol); Experimental group 2: (200mg/kg + 400mg/kg paracetamol), Experimental group 3: (400mg/kg + 400mg/kg paracetamol), after 10 days the animals were sacrificed, a liver sample was extracted for the study histopathological, histopathological indicators (type and extent of the lesion) were evaluated. Results: The integument of Raphanus sativus L. is soluble in water, methanol and ethanol. The phytochemical study revealed the presence of flavonoids, tannins, alkaloids, steroids. Regarding the type and extent of the lesion at the histopathological level, it was mild. hepatic and focal degeneration in experimental groups 2 and 3. Conclusion: According to the histopathological analysis, a dose of 200 and 400 mg/kg of the ethanolic extract of the integument of Raphanus Sativus L. “rabanito” presented hepatoprotective effects on paracetamol-induced hepatotoxicity. 400mg/kg.