Examinando por Autor "Carhuapoma Yance, Mario"

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Acceso abierto
Actividad antimicrobiana del aceite esencial de Origanum vulgare L. “orégano peruano” frente a Staphylococcus aureus
(Universidad Privada Norbert Wiener, 2018-05-31) Salinas Segura, Roger; Carhuapoma Yance, Mario
The objective of this research was to confirm the antimicrobial activity of the essential oil of Origanum vulgare L., "Peruvian Oregano," against Staphylococcus aureus. The essential oil was obtained by steam distillation and purified with anhydrous sodium sulfate. The analysis of the main phytochemical components of the oil was performed using organoleptic and instrumental methods. The antimicrobial activity was assayed against Staphylococcus aureus ATCC 6538 using the disk diffusion method (Kirby-Bauer), with Gentamicin 10 µg (OXOID brand) as the positive control and dimethyl sulfoxide as the negative control. Results: The essential oil had a good yield of 2.02%, distilled in 60 minutes, with a density of 0.9132 g/mL and a refractive index of 1.475. The main components were carvacrol and thymol. The essential oil of Origanum vulgare L. showed antimicrobial activity against Staphylococcus aureus ATCC 6538, with inhibition halos of 11.47,13.40,16.60, and 19.65 mm at concentrations of 25,50,75, and 100 µg/mL, respectively. The gentamicin control resulted in an inhibition halo of 30.14 mm. Conclusion: The highest antimicrobial activity of the essential oil of Origanum vulgare L. "Peruvian Oregano" against Staphylococcus aureus was confirmed at 100 µg/mL.
Acceso abierto
Buenas prácticas de distribución y transporte, manteniendo la cadena de frío 2°C a 8°C en productos terminados de Paclitaxel Paclitex®, Toxina Botulínica Cunox® y Octreotide Azestan®
(Universidad Privada Norbert Wiener, 2015) Moreto Riofrio, Oscar Reinaldo; Carhuapoma Yance, Mario
In the preservation of the cold chain, the weakest and most critical stage is undoubtedly transportation during the distribution of finished products. It is essential to identify suitable materials to maintain the cold chain through a series of tests, which must be documented to demonstrate that a certain quantity of Gel Ice Packs combined with appropriately sized Cold Boxes ensures temperature control within a predefined period. This guarantees compliance with good distribution and transportation practices. In this study, a method was developed to ensure the proper distribution and conservation of finished pharmaceutical products requiring a specific temperature of 2°C to 8°C to maintain their stability. Cold Boxes from different suppliers were purchased, evaluated, and compared to assess their performance in preserving the cold chain. Gel Ice Packs, accompanied by certificates describing their chemical composition and freezing point, were analyzed and approved by an authorized health network laboratory. Storage equipment and facilities used for products such as Paclitaxel PACLITEX®, Botulinum Toxin CUNOX®, and Octreotide AZESTAN® were calibrated, certified, and maintained under a periodic maintenance program. Field tests were conducted in three provinces with extreme seasonal temperatures. Based on the results of the first and second phases, an instructional guide was prepared for proper product packaging. Finally, the fourth and fifth tests were carried out in Callao and Lima, respectively, using optimal quantities and sizes of materials, with satisfactory results confirmed by temperature controllers (Temptale4) graphs.
Acceso abierto
Determinación fitoquímica y actividad antimicrobiana del aceite esencial de los órganos aéreos de Lepechinia meyenii (Walp.) Epling "pacha salvia"
(Universidad Privada Norbert Wiener, 2018-03-09) Quispe Silva, Mirela Magaly; Tenorio Roca, Eduardo; Carhuapoma Yance, Mario
The plant species Lepechinia meyenii (Walp.) Epling (commonly known as salvia, pacha salvia, salvia de puna, kotapuriña) is a native plant that grows in the southern Andean regions of Peru (Ayacucho, Huancavelica, Cuzco, and Puno). The main objective of the present study was to conduct a phytochemical evaluation and determine the antimicrobial activity of the essential oil obtained from the aerial parts of Lepechinia meyenii (Walp.) Epling “pacha salvia.” In the methodology, a gas chromatograph coupled with a mass spectrometer (GC-MS) was employed to determine the phytochemical composition, and the agar diffusion method (Kirby-Bauer) was used to evaluate antimicrobial activity. The results showed the presence of 31 components, with the major compounds being α-pinene (21.37%), eucalyptol (1,8-cineole = 18.80%), and D-limonene (15.43%). The essential oil demonstrated antimicrobial activity against Staphylococcus aureus ATCC 6538 when compared with gentamicin 10 µg. It is concluded that the essential oil from the aerial parts of Lepechinia meyenii (Walp.) Epling “pacha salvia” contains active components with antimicrobial activity.