Examinando por Autor "Carhuaricra Soto, Yudiht Abigail"

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(Universidad Privada Norbert Wiener, 2015) Carhuaricra Soto, Yudiht Abigail; Malpartida Quispe, Federico Martin
In periodontal disease, various microorganisms are involved, one of which is Porphyromonas gingivalis, an obligate anaerobic, Gram-negative bacterium that is predominantly found in chronic periodontitis, and is commonly eliminated by 0.12% chlorhexidine. The purpose of this study was to determine the inhibitory effect of Rosmarinus officinalis (rosemary) essential oil and 0.12% chlorhexidine on Porphyromonas gingivalis, using the agar diffusion method with discs, as an effort to find an alternative, natural, effective, and economical antimicrobial substance against this bacterium. For this, Porphyromonas gingivalis strains were inoculated in 40 Petri dishes containing Mueller Hinton agar. Then, three discs were placed on each plate, impregnated with 20 µl of 0.12% chlorhexidine, Rosmarinus officinalis essential oil, and distilled water as a negative control. The plates were incubated at 37°C in an anaerobic jar for the duration of the study, being removed only to measure the inhibition halos generated at 24 and 48 hours. The data were processed using SPSS software, and the Student's T-test was applied. It was concluded that Rosmarinus officinalis (rosemary) essential oil exhibited an inhibitory effect against Porphyromonas gingivalis, and 0.12% chlorhexidine had a greater inhibitory effect than the essential oil against this bacterial strain.