Examinando por Autor "Chávez Hidalgo, Diego Andrés"

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(Universidad Privada Norbert Wiener, 2017-09-29) Chávez Hidalgo, Diego Andrés; Cupé Araujo, Ana Cecilia
Materials and Methods: Ten toothpaste brands available in the local market were used, with pediatric toothpastes recommended by specialists in pediatric dentistry. Streptococcus mutans (ATCC 25175) was cultured on a specific medium (tryptic soy agar, yeast extract, sucrose, and bacitracin), which is ideal for the recovery and evaluation of Streptococcus mutans, as referenced in studies certifying its suitability. Bacitracin was used to allow only the growth of this bacterium, preventing contamination in our cultures. The agar diffusion method was used, where the agar is inoculated with the bacterial strain to ensure uniform growth. A modification was made to the preparation of the agar, adding 50 ml of sterile lamb blood to create contrast between the agar and inhibition halos. The cylinder-plate technique from the United States Pharmacopeia was employed, which involves using calibrated cylinders (made of surgical steel or porcelain) measuring 10 mm in height and having an external diameter of 8 mm and an internal diameter of 6 mm. These cylinders were placed on the plates with a spacing of 6 cylinders per plate, following the plate's perimeter and ensuring distance between them for the formation of inhibition halos. According to the technique's protocol, 6 Petri dishes were used for each toothpaste evaluated, 6 plates for evaluating the Gold Standard (penicillin plus gentamicin), and another 6 Petri dishes for the negative evaluation. Conclusions: The toothpaste brands sold in the local market showed inhibitory activity against Streptococcus mutans (ATCC 25175), except for one.