Examinando por Autor "Davies, Neal M."

Mostrando 1 - 14 de 14

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Calidad de vida, ansiedad y depresión en pacientes peruanos con síndrome coronario agudo
(MDPI, 2022-11-11) Furlong-Millones, Marco R.; Mostacero-Becerra, Katherin; Aguirre-Milachay, Edwin; Álvarez-Risco, Aldo; Del-Aguila-Arcentales, Shyla; garcia guerra, Andres; Davies, Neal M.; Yáñez, Jaime A.; Valladares Garrido, Mario J.
El presente estudio tiene como objetivo identificar los factores asociados a la ansiedad y depresión en pacientes con síndrome coronario agudo (SCA) al alta hospitalaria de un centro de salud peruano. Los pacientes al alta del servicio de hospitalización de cardiología entre noviembre de 2019 y diciembre de 2020 fueron evaluados mediante un estudio transversal. La mediana de tiempo transcurrido desde el evento de SCA hasta la fecha de la entrevista fue de 10 meses. El 34,1% de la población presentó depresión leve y el 78,8% ansiedad leve. Nuestros tres análisis indicaron que los pacientes que habían asistido a la universidad tenían niveles significativamente más bajos tanto de depresión como de ansiedad, y los pacientes con diabetes tenían niveles significativamente más altos de ansiedad. El grupo socioeconómico bajo-bajo tuvo 1,5 veces más frecuencia de depresión ( p-valor = 0,002) y 3,12 veces la frecuencia de la ansiedad ( p -valor = 0,050). Curiosamente, mientras que una buena calidad de vida se asoció con niveles más bajos de depresión, también se asoció con niveles más altos de ansiedad moderada/grave ( valor de p = 0,035). Un análisis de regresión múltiple encontró que la hipertensión también se asoció con niveles más altos de ansiedad, y los pacientes que habían tenido COVID-19 tenían 21,05 veces el nivel de ansiedad moderada/grave ( valor de p = 0,000). Los casos de SCA son más frecuentes en pacientes con una edad mayor o igual a 60 años, así como en el sexo masculino. El aislamiento fue una característica común que puede tener un impacto negativo en su calidad de vida y salud mental.
Acceso abierto
Calidad de vida, ansiedad y depresión en pacientes peruanos con síndrome coronario agudo
(MDPI, 2022-11-11) Furlong-Millones, Marco R.; Mostacero-Becerra, Katherin; Aguirre-Milachay, Edwin; Álvarez-Risco, Aldo; Del-Aguila-Arcentales, Shyla; garcia guerra, Andres; Davies, Neal M.; Yáñez, Jaime A.; Valladares Garrido, Mario J.
El presente estudio tiene como objetivo identificar los factores asociados a la ansiedad y depresión en pacientes con síndrome coronario agudo (SCA) al alta hospitalaria de un centro de salud peruano. Los pacientes al alta del servicio de hospitalización de cardiología entre noviembre de 2019 y diciembre de 2020 fueron evaluados mediante un estudio transversal. La mediana de tiempo transcurrido desde el evento de SCA hasta la fecha de la entrevista fue de 10 meses. El 34,1% de la población presentó depresión leve y el 78,8% ansiedad leve. Nuestros tres análisis indicaron que los pacientes que habían asistido a la universidad tenían niveles significativamente más bajos tanto de depresión como de ansiedad, y los pacientes con diabetes tenían niveles significativamente más altos de ansiedad. El grupo socioeconómico bajo-bajo tuvo 1,5 veces más frecuencia de depresión ( p-valor = 0,002) y 3,12 veces la frecuencia de la ansiedad ( p -valor = 0,050). Curiosamente, mientras que una buena calidad de vida se asoció con niveles más bajos de depresión, también se asoció con niveles más altos de ansiedad moderada/grave ( valor de p = 0,035). Un análisis de regresión múltiple encontró que la hipertensión también se asoció con niveles más altos de ansiedad, y los pacientes que habían tenido COVID-19 tenían 21,05 veces el nivel de ansiedad moderada/grave ( valor de p = 0,000). Los casos de SCA son más frecuentes en pacientes con una edad mayor o igual a 60 años, así como en el sexo masculino. El aislamiento fue una característica común que puede tener un impacto negativo en su calidad de vida y salud mental.
Acceso abierto
Consumer Patterns of Sustainable Clothing Based on Theory of Reasoned Action: Evidence from Ecuador
(Sustainability (Switzerland), 2022-11-09) Leclercq-Machado, Luigi; Alvarez-Risco, Aldo; García-Ibarra, Verónica; Esquerre-Botton, Sharon; Morales-Ríos, Flavio; Anderson-Seminario, Maria de las Mercedes; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Yáñez, Jaime A.
Corporations need to understand the factors that influence purchase intention. The current study aimed to understand sustainable clothing patterns in Ecuador. A total of 343 Ecuadorian consumers completed an online survey; the results were analyzed with partial least squares structural equation modeling (PLS-SEM). As the outcome, attitude was predicted by perceived environmental knowledge (PEK) and environmental concern (EC). PEK and EC are positively correlated to attitudes towards purchasing sustainable clothing. Additionally, attitude mediated the relationship between these two variables and purchase intention. As measured by PEK, attitude is the most critical factor in determining purchase intention, based on importance performance map analysis (IPMA). The research findings may support firms’ marketing and selling strategies to demonstrate that their brands are environmentally green and generate greater consumer interest in current and future customers. The novelty of these findings is supported by the partial least squares structural equation modeling (PLS-SEM) technique results.
Acceso abierto
Consumer Patterns of Sustainable Clothing Based on Theory of Reasoned Action: Evidence from Ecuador
(Sustainability (Switzerland), 2022-11-09) Leclercq-Machado, Luigi; Alvarez-Risco, Aldo; García-Ibarra, Verónica; Esquerre-Botton, Sharon; Morales-Ríos, Flavio; Anderson-Seminario, Maria de las Mercedes; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Yáñez, Jaime A.
Corporations need to understand the factors that influence purchase intention. The current study aimed to understand sustainable clothing patterns in Ecuador. A total of 343 Ecuadorian consumers completed an online survey; the results were analyzed with partial least squares structural equation modeling (PLS-SEM). As the outcome, attitude was predicted by perceived environmental knowledge (PEK) and environmental concern (EC). PEK and EC are positively correlated to attitudes towards purchasing sustainable clothing. Additionally, attitude mediated the relationship between these two variables and purchase intention. As measured by PEK, attitude is the most critical factor in determining purchase intention, based on importance performance map analysis (IPMA). The research findings may support firms’ marketing and selling strategies to demonstrate that their brands are environmentally green and generate greater consumer interest in current and future customers. The novelty of these findings is supported by the partial least squares structural equation modeling (PLS-SEM) technique results.
Acceso abierto
Factors Associated with Mortality in Patients with COVID-19 from a Hospital in Northern Peru
(MDPI, 2023-03-23) Valladares-Garrido, Mario J.; Alvarez-Risco, Aldo; Vasquez-Elera, Luis E.; Valdiviezo-Morales, Christopher G.; Martinez-Rivera, Raisa N.; Cruz-Zapata, Annel L.; Pereira-Victorio, César Johan; Garcia-Peña, Elian; Failoc-Rojas, Virgilio E.; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Yáñez, Jaime A.
We aimed to identify the factors associated with mortality in patients with COVID-19 from the hospitalization service of the Cayetano Heredia Hospital, Piura, Peru, from May to June 2020. A prospective study was conducted in hospitalized patients with a confirmed diagnosis of COVID-19 through serological and/or molecular reactive testing. The dependent variable was death due to COVID-19, and the independent variables were the epidemiological, clinical and laboratory characteristics of the patient. The chi-square test and the non-parametric Mann–Whitney U test were used, with a significance level of 5%. Of 301 patients with COVID-19, the majority of them were male (66.1%), and the mean age was 58.63 years. Of the patients analyzed, 41.3% of them died, 40.2% of them were obese and 59.8% of them had hepatic steatosis. The three most frequent signs/symptoms were dyspnea (90.03%), fatigue (90.03%) and a cough (84.72%). Being an older adult (p = 0.011), being hospitalized in the ICU (p = 0.001), overweight (p = 0.016), obese (p = 0.021) and having compromised consciousness (p = 0.039) and thrombocytopenia (p = 0.024) were associated with mortality due to COVID-19. Overall, the mortality rate due to COVID-19 was 41.3%. Having an older age, being hospitalized in the ICU, overweight, obese and having compromised consciousness and thrombocytopenia were positively associated with mortality in patients with COVID-19. These findings highlight the need to establish an adequate system of surveillance and epidemiological education in hospitals and communities in the event of new outbreaks, especially in rural and northern Peru.
Acceso abierto
Factors Associated with Mortality in Patients with COVID-19 from a Hospital in Northern Peru
(MDPI, 2023-02-23) Valladares-Garrido, Mario J.; Alvarez-Risco, Aldo; Vasquez-Elera, Luis E.; Valdiviezo-Morales, Christopher G.; Martinez-Rivera, Raisa N.; Cruz-Zapata, Annel L.; Pereira-Victorio, César Johan; Garcia-Peña, Elian; Failoc-Rojas, Virgilio E.; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Yáñez, Jaime A.
We aimed to identify the factors associated with mortality in patients with COVID-19 from the hospitalization service of the Cayetano Heredia Hospital, Piura, Peru, from May to June 2020. A prospective study was conducted in hospitalized patients with a confirmed diagnosis of COVID-19 through serological and/or molecular reactive testing. The dependent variable was death due to COVID-19, and the independent variables were the epidemiological, clinical and laboratory characteristics of the patient. The chi-square test and the non-parametric Mann–Whitney U test were used, with a significance level of 5%. Of 301 patients with COVID-19, the majority of them were male (66.1%), and the mean age was 58.63 years. Of the patients analyzed, 41.3% of them died, 40.2% of them were obese and 59.8% of them had hepatic steatosis. The three most frequent signs/symptoms were dyspnea (90.03%), fatigue (90.03%) and a cough (84.72%). Being an older adult (p = 0.011), being hospitalized in the ICU (p = 0.001), overweight (p = 0.016), obese (p = 0.021) and having compromised consciousness (p = 0.039) and thrombocytopenia (p = 0.024) were associated with mortality due to COVID-19. Overall, the mortality rate due to COVID-19 was 41.3%. Having an older age, being hospitalized in the ICU, overweight, obese and having compromised consciousness and thrombocytopenia were positively associated with mortality in patients with COVID-19. These findings highlight the need to establish an adequate system of surveillance and epidemiological education in hospitals and communities in the event of new outbreaks, especially in rural and northern Peru.
Acceso abierto
Factors Associated with SARS-CoV-2 Positivity in Patients Treated at the Lambayeque Regional Hospital, Peru during a Pandemic Period
(MDPI, 2022-10-18) Valladares-Garrido, Mario J.; Alvarez-Risco, Aldo; Rojas-Alvarado, Annel B.; Zuniga-Cáceres, José A.; Estrella Izarra, Naylamp A.; Christopher Ichiro Peralta; Astudillo, David; Díaz-Vélez,, Cristian; Failoc Rojas, Virgilio E.; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Garcia Guerra, Andrés; Yáñez, Jaime A.
The aim of this study was to provide additional data on mortality from COVID-19 with particular attention to the factors associated with the positivity of patients admitted to the Lambayeque Hospital in Peru. A retrospective cohort analysis was carried out to determine the clinical-epidemiological factors associated with positivity for SARS-CoV-2 in patients treated at the Lambayeque Regional Hospital during the health emergency period in the context of the COVID-19 pandemic. It was observed that, as the demographic age group increased, the percentage of seropositivity increased, with 66.8% of elderly adults testing positive, compared to 37.4% of children (p < 0.001). More seropositive men than women were evident (61.1% vs. 54.1%; p < 0.001). The most frequent symptom of patients with suspected COVID-19 was cough (65.0%). However, the symptoms with the greatest frequency of seropositive patients were ageusia (78.6%) and fever (77.6%); cough was one of the symptoms with the lowest (63.9%) (p-value < 0.001). The comorbidities with the most seropositive patients were obesity (80.7%) and diabetes mellitus (73.6%) (p-value < 0.001), different from the top comorbidity of heart disease (12.7%) in suspected COVID-19 patients. In terms of disease signs, abnormal findings on MRI (98.11%) and dyspnea (28.7%) were the most common in suspected COVID-19 patients, similar to those in seropositive patients, which were dyspnea (81.4%) and abnormal tomography findings (75.3%) (p-value < 0.001).
Acceso abierto
Factors Associated with SARS-CoV-2 Positivity in Patients Treated at the Lambayeque Regional Hospital, Peru during a Pandemic Period
(MDPI, 2022-10-18) Valladares-Garrido, Mario J.; Alvarez-Risco, Aldo; Rojas-Alvarado, Annel B.; Zuniga-Cáceres, José A.; Estrella Izarra, Naylamp A.; Christopher Ichiro Peralta; Astudillo, David; Díaz-Vélez,, Cristian; Failoc Rojas, Virgilio E.; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Garcia Guerra, Andrés; Yáñez, Jaime A.
The aim of this study was to provide additional data on mortality from COVID-19 with particular attention to the factors associated with the positivity of patients admitted to the Lambayeque Hospital in Peru. A retrospective cohort analysis was carried out to determine the clinical-epidemiological factors associated with positivity for SARS-CoV-2 in patients treated at the Lambayeque Regional Hospital during the health emergency period in the context of the COVID-19 pandemic. It was observed that, as the demographic age group increased, the percentage of seropositivity increased, with 66.8% of elderly adults testing positive, compared to 37.4% of children (p < 0.001). More seropositive men than women were evident (61.1% vs. 54.1%; p < 0.001). The most frequent symptom of patients with suspected COVID-19 was cough (65.0%). However, the symptoms with the greatest frequency of seropositive patients were ageusia (78.6%) and fever (77.6%); cough was one of the symptoms with the lowest (63.9%) (p-value < 0.001). The comorbidities with the most seropositive patients were obesity (80.7%) and diabetes mellitus (73.6%) (p-value < 0.001), different from the top comorbidity of heart disease (12.7%) in suspected COVID-19 patients. In terms of disease signs, abnormal findings on MRI (98.11%) and dyspnea (28.7%) were the most common in suspected COVID-19 patients, similar to those in seropositive patients, which were dyspnea (81.4%) and abnormal tomography findings (75.3%) (p-value < 0.001).
Acceso abierto
Green Synthesis of a Novel Silver Nanoparticle Conjugated with Thelypteris glandulosolanosa (Raqui-Raqui): Preliminary Characterization and Anticancer Activity
(MDPI, 2022-06-21) Vera-Nuñez, Lucero Del Carmen; Cornejo-Ruiz, Junior Oliver; Arenas-Chávez, Carlos Alberto; de Hollanda, Luciana Maria; Alvarez-Risco, Aldo; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Yáñez, Jaime A.; Vera-Gonzales, Corina
“In the last decade, the green synthesis of nanoparticles has had a prominent role in scientific research for industrial and biomedical applications. In this current study, silver nitrate (AgNO3 ) was reduced and stabilized with an aqueous extract of Thelypteris glandulosolanosa (Raqui-raqui), forming silver nanoparticles (AgNPs-RR). UV-vis spectrophotometry, dynamic light scattering (DLS), and scanning transmission electron microscopy (STEM) were utilized to analyze the structures of AgNPsRR. The results from this analysis showed a characteristic peak at 420 nm and a mean hydrodynamic size equal to 39.16 nm, while the STEM revealed a size distribution of 6.64–51.00 nm with an average diameter of 31.45 nm. Cellular cytotoxicity assays using MCF-7 (ATCC® HTB-22™, mammary gland breast), A549 (ATCC® CCL-185, lung epithelial carcinoma), and L929 (ATCC® CCL-1, subcutaneous connective tissue of Mus musculus) demonstrated over 42.70% of MCF-7, 59.24% of A549, and 8.80% of L929 cells had cell death after 48 h showing that this nanoparticle is more selective to disrupt neoplastic than non-cancerous cells and may be further developed into an effective strategy for breast and lung cancer treatment. These results demonstrate that the nanoparticle surfaces developed are complex, have lower contact angles, and have excellent scratch and wear resistance.“
Acceso abierto
Green Synthesis of a Novel Silver Nanoparticle Conjugated with Thelypteris glandulosolanosa (Raqui-Raqui): Preliminary Characterization and Anticancer Activity
(MDPI, 2022-06-21) Vera-Nuñez, Lucero Del Carmen; Cornejo-Ruiz, Junior Oliver; Arenas-Chávez, Carlos Alberto; de Hollanda, Luciana Maria; Alvarez-Risco, Aldo; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Yáñez, Jaime A.; Vera-Gonzales, Corina
“In the last decade, the green synthesis of nanoparticles has had a prominent role in scientific research for industrial and biomedical applications. In this current study, silver nitrate (AgNO3 ) was reduced and stabilized with an aqueous extract of Thelypteris glandulosolanosa (Raqui-raqui), forming silver nanoparticles (AgNPs-RR). UV-vis spectrophotometry, dynamic light scattering (DLS), and scanning transmission electron microscopy (STEM) were utilized to analyze the structures of AgNPsRR. The results from this analysis showed a characteristic peak at 420 nm and a mean hydrodynamic size equal to 39.16 nm, while the STEM revealed a size distribution of 6.64–51.00 nm with an average diameter of 31.45 nm. Cellular cytotoxicity assays using MCF-7 (ATCC® HTB-22™, mammary gland breast), A549 (ATCC® CCL-185, lung epithelial carcinoma), and L929 (ATCC® CCL-1, subcutaneous connective tissue of Mus musculus) demonstrated over 42.70% of MCF-7, 59.24% of A549, and 8.80% of L929 cells had cell death after 48 h showing that this nanoparticle is more selective to disrupt neoplastic than non-cancerous cells and may be further developed into an effective strategy for breast and lung cancer treatment. These results demonstrate that the nanoparticle surfaces developed are complex, have lower contact angles, and have excellent scratch and wear resistance.“
Acceso abierto
Optimization of Synthesis of Silver Nanoparticles Conjugated with Lepechinia meyenii (Salvia) Using Plackett-Burman Design and Response Surface Methodology—Preliminary Antibacterial Activity
(MDPI, 2022-08-31) Laime-Oviedo, Luis Alberto; Soncco-Ccahui, Amanda Allison; Peralta-Alarcon, Gladis; Arenas-Chávez, Carlos Alberto; Pineda-Tapia, Jose Luis; Díaz-Rosado , José Carlos; Alvarez-Risco, Aldo; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Yáñez, Jaime A.; Vera-Gonzales , Corina
“In the present investigation, an ethanolic fraction (EF) of Lepechinia meyenii (salvia) was prepared and fractionated by gradient column chromatography, and the main secondary metabolites present in the EF were identified by HPLC-MS. Silver nanoparticles (AgNPs) were synthesized and conjugated with the EF of Lepechinia meyenii (salvia). The AgNPs synthesis was optimized using Plackett-Burman design and response surface methodology (RSM), considering the following independent variables: stirring speed, synthesis pH, synthesis time, synthesis temperature and EF volume. The AgNPs synthesized under the optimized conditions were characterized by UV visible spectroscopy (UV-VIS), Fourier Transform Infrared Spectroscopy (FT-IR), Dynamic Light Scattering (DLS) and Scanning Transmission Electron Microscopy (STEM). The antibacterial activity of the AgNPs against Staphylococcus aureus (ATCC® 25923) was evaluated. The following flavonoids were identified: rosmarinic acid, diosmin and hesperetin-7-O-rutinoside. The optimized conditions for the synthesis of nanoparticles were pH 9.45, temperature 49.8 ◦C, volume of ethanolic fraction 152.6 µL and a reaction time of 213.2 min. The obtained AgNPs exhibited an average size of 43.71 nm and a resonance plasmon of 410–420 nm. Using FT-IR spectroscopy, the disappearance of the peaks between 626.50 and 1379.54 cm−1 was evident with the AgNPs, which would indicate the participation of these functional groups in the synthesis and protection of the nanoparticles. A hydrodynamic size of 47.6 nm was obtained by DLS, while a size of 40–60 nm was determined by STEM. The synthesized AgNPs conjugated with the EF showed a higher antibacterial activity than the EF alone. These results demonstrate that the AgNPs synthesized under optimized conditions conjugated with the EF of the Lepechinia meyenii (salvia) presented an increased antibacterial activity.“
Acceso abierto
Optimization of Synthesis of Silver Nanoparticles Conjugated with Lepechinia meyenii (Salvia) Using Plackett-Burman Design and Response Surface Methodology—Preliminary Antibacterial Activity
(MDPI, 2022-08-31) Laime-Oviedo, Luis Alberto; Soncco-Ccahui, Amanda Allison; Peralta-Alarcon, Gladis; Arenas-Chávez, Carlos Alberto; Pineda-Tapia, Jose Luis; Díaz-Rosado, José Carlos; Alvarez-Risco , Aldo; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Yáñez, Jaime A.; Vera-Gonzales, Corina
“In the present investigation, an ethanolic fraction (EF) of Lepechinia meyenii (salvia) was prepared and fractionated by gradient column chromatography, and the main secondary metabolites present in the EF were identified by HPLC-MS. Silver nanoparticles (AgNPs) were synthesized and conjugated with the EF of Lepechinia meyenii (salvia). The AgNPs synthesis was optimized using Plackett-Burman design and response surface methodology (RSM), considering the following independent variables: stirring speed, synthesis pH, synthesis time, synthesis temperature and EF volume. The AgNPs synthesized under the optimized conditions were characterized by UV visible spectroscopy (UV-VIS), Fourier Transform Infrared Spectroscopy (FT-IR), Dynamic Light Scattering (DLS) and Scanning Transmission Electron Microscopy (STEM). The antibacterial activity of the AgNPs against Staphylococcus aureus (ATCC® 25923) was evaluated. The following flavonoids were identified: rosmarinic acid, diosmin and hesperetin-7-O-rutinoside. The optimized conditions for the synthesis of nanoparticles were pH 9.45, temperature 49.8 ◦C, volume of ethanolic fraction 152.6 µL and a reaction time of 213.2 min. The obtained AgNPs exhibited an average size of 43.71 nm and a resonance plasmon of 410–420 nm. Using FT-IR spectroscopy, the disappearance of the peaks between 626.50 and 1379.54 cm−1 was evident with the AgNPs, which would indicate the participation of these functional groups in the synthesis and protection of the nanoparticles. A hydrodynamic size of 47.6 nm was obtained by DLS, while a size of 40–60 nm was determined by STEM. The synthesized AgNPs conjugated with the EF showed a higher antibacterial activity than the EF alone. These results demonstrate that the AgNPs synthesized under optimized conditions conjugated with the EF of the Lepechinia meyenii (salvia) presented an increased antibacterial activity.“
Acceso abierto
Purification and Characterization of a Novel Factor of Crotoxin Inter-CRO (V-1), a New Phospholipase A2 Isoform from Crotalus durissus collilineatus Snake Venom Using an In Vitro Neuromuscular Preparation
(MDPI, 2022-07-21) Vera-Gonzales, Corina; Arenas-Chávez, Carlos Alberto; Ponce-Soto, Luis A.; Alvarez-Risco, Aldo; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Yáñez, Jaime A.
The fractionation of Crotalus durissus collilineatus whole venom through an HPLC chromatographic method enabled the purification of a new V-1 neurotoxin. Inter-CRO (V-1) presents similarity in its primary structure to crotoxin B (CB), suggesting another isoform of this toxin. The aim of this study was to compare V-1 to the crotoxin complex (CA/CB) and CB to elucidate aspects related to its functionality. The homogeneity of the purified protein was confirmed with a molecular mass of 1425.45 Da, further verified by mass spectrometry. The sequence of the protein showed high similarity to other viperid snake venom PLA2 proteins. The results of this study report that V-1 is an uncharacterized novel toxin with different biological activities from CB. V-1 maintained catalytic activity but presented neurotoxic activity as observed by the 2.5-fold increase in twitch tension record compared to control values on isolated muscle cells. View Full-Text
Acceso abierto
Purification and Characterization of a Novel Factor of Crotoxin Inter-CRO (V-1), a New Phospholipase A2 Isoform from Crotalus durissus collilineatus Snake Venom Using an In Vitro Neuromuscular Preparation
(MDPI, 2022-07-21) Vera-Gonzales, Corina; Arenas-Chávez , Carlos Alberto; Ponce-Soto, Luis A.; Alvarez-Risco, Aldo; Del-Aguila-Arcentales, Shyla; Davies, Neal M.; Yáñez , Jaime A.
“The fractionation of Crotalus durissus collilineatus whole venom through an HPLC chromatographic method enabled the purification of a new V-1 neurotoxin. Inter-CRO (V-1) presents similarity in its primary structure to crotoxin B (CB), suggesting another isoform of this toxin. The aim of this study was to compare V-1 to the crotoxin complex (CA/CB) and CB to elucidate aspects related to its functionality. The homogeneity of the purified protein was confirmed with a molecular mass of 1425.45 Da, further verified by mass spectrometry. The sequence of the protein showed high similarity to other viperid snake venom PLA2 proteins. The results of this study report that V-1 is an uncharacterized novel toxin with different biological activities from CB. V-1 maintained catalytic activity but presented neurotoxic activity as observed by the 2.5-fold increase in twitch tension record compared to control values on isolated muscle cells.“