Examinando por Autor "Fortuna García, Edgar Fernando"

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Evaluación de la toxicidad aguda del extracto hidroalcohólico de hojas frescas de Mutisia acuminata R. & P. “chinchilcuma” en cerebros y cerebelos de ratas albinas cepa Holtzmann
(Universidad Privada Norbert Wiener, 2013) Fortuna García, Edgar Fernando; Chávez Flores, Juana Elvira
The plant species Mutisia acuminata R. & P. “chinchilcuma,” collected in the community of Huaruccña (located at 3200 m.a.s.l.), district of Coracora, province of Parinacochas, department of Ayacucho (Peru), was taken to the Pharmacology Laboratory of the Norbert Wiener Private University in June 2011 for the evaluation of the acute toxicity of the hydroalcoholic extract of fresh leaves in Holtzman albino rats. In the phytochemical analysis, the presence of eight metabolites was determined. For the lethality test (5000 mg/kg, LD50), 20 rats were administered, via intragastric route and in a single dose, different volumes of the extract according to their body weight. Four rats were only given distilled water. The experimental animals were kept under observation for 14 days, at the end of which no signs of toxicity, alteration of the growth curve of the species, or death of any animal were detected. On day 15, euthanasia was performed, and the encephalic organs were removed to carry out the anatomopathological analysis of brain and cerebellum tissues. No macroscopic lesions were found. Microscopic observations revealed mild, moderate, and severe edema; mild congestion in one female rat; and severe congestion in one male rat. According to the Globally Harmonized System (GHS), the hydroalcoholic extract of the evaluated botanical species at 5000 mg/kg is considered “practically non-toxic.”