Examinando por Autor "Guevara Vicaña, Rita Aurora"

Mostrando 1 - 4 de 4

Acceso abierto
Determinación de la calidad microbiológica e inocuidad del agua potable para consumo de los dispensadores de las boticas y farmacias del distrito de Breña en el departamento de Lima, mayo-junio de 2017
(Universidad Privada Norbert Wiener, 2017) Bobadilla Poquioma, Carlos Yoel; Hurtado Cahuana, José Luis; Guevara Vicaña, Rita Aurora
Water intended for consumption, the preparation of food and beverages, or personal hygiene must not contain any human pathogens. This can be achieved by selecting high-quality water sources, effectively treating and decontaminating water polluted by human or animal feces or other substances, and protecting it from contamination during distribution to the user. Therefore, it is necessary to determine the presence of microorganisms that indicate water safety (total coliforms) and those that indicate water microbiological quality (Heterotrophs). This was carried out on samples from 67 pharmaceutical establishments in the Breña district during May–June 2017. The determination of coliform microorganisms was performed by the multiple tube method, and the determination of heterotrophic microorganisms was performed by the pour plate count method. The results were compared with the standards established in the Regulation on Drinking Water Quality: D.S. N° 031-2010-SA / Ministry of Health. General Directorate of Environmental Health – Lima: Ministry of Health; 2011. 4.5% of the samples did not comply with the established parameters (Heterotrophs), as the maximum permissible limit according to current national standards is 500 CFU/mL of sample.
Acceso abierto
Determinación de la calidad sanitaria e inocuidad de salsas picantes servidas en establecimientos comerciales de la urbanización Santa Anita en el distrito de Santa Anita – Lima abril 2018
(Universidad Privada Norbert Wiener, 2019-06-26) Rosales Ñaupari, Ketty Inés; Guevara Vicaña, Rita Aurora
The objective of this study was to evaluate the level of sanitary quality and safety of hot sauces served in commercial establishments in the Santa Anita Urbanization in the Santa Anita District. For this purpose, a total of 30 hot sauce samples were analyzed: 10 from market stalls (0-fork category), 10 from restaurants (1-fork category), and 10 from restaurants (2-fork category). The sampling was non-probabilistic and by convenience. Method: The 3M Petrifilm Plates for Mold and Yeast Count and for Total Coliform Count (AOAC 991.14) were used. For data interpretation, the limits established by Sanitary Technical Standard NTS No. 071 – MINSA/DIGESA - V.01 were applied. Results: Of the establishments studied, 1 out of 10 (10.00%) in the 0-fork category, 2 out of 10 (20.00%) in the 1-fork category, and 2 out of 10 (20.00%) in the 2-fork category complied with the microbiological criteria. Conclusion: The commercial establishments in the Santa Anita Urbanization have low sanitary quality and safety in the hot sauces served, according to the permissible values of microbiological indicators established in the aforementioned standard.
Acceso abierto
Implementación del método de filtración por membrana empleando el sistema de filtración EZ-FIT™ en análisis microbiológico de agua purificada en laboratorio de caramelos farmacéuticos en el periodo de mayo–octubre del 2019
(Universidad Privada Norbert Wiener, 2020-11-27) Minaya Laureano, Maribel Florencia; Guevara Vicaña, Rita Aurora
The objective of this thesis was to compare the implementation of the membrane filtration method using the EZ-FITTM filtration system in a pharmaceutical candy laboratory for the analysis of purified water. The research design was observational, analytical, and descriptive. The study confirmed that the percentage of recovery for the inoculated microorganisms was greater than 70%. It was also evident that the data had a normal distribution, which allowed for the use of the Student's t-test to compare the results of both methods, with a 95% confidence level for the intervals of microbial counts. The comparison was made between the TSA agar medium method and the EZ-FITTM membrane filtration method. This work determined that the mean differences in CFU recovery for E. coli, P. aeruginosa, and S. aureus cultures were not statistically significant, meaning that either technique would provide satisfactory results. Ultimately, according to its established procedures, the laboratory decides which method to apply, prioritizing the benefits of its management.
Acceso abierto
Validación del método microbiológico de cilindro-placa para la cuantificación de gentamicina sulfato en Dermolab NF crema de Laboratorios Vitapharma S.A.C., Lima 2018
(Universidad Privada Norbert Wiener, 2019-06-11) Huerto Huapaya, Carmen Aracelli; Vignolo Asencio, Luis Ernesto; Guevara Vicaña, Rita Aurora
The present study was conducted with the objective of standardizing a microbiological cylinder-plate method for the quantification of gentamicin and subsequently validating it, that is, to demonstrate through documented evidence in the Quality Control Laboratory of Vitapharma S.A.C. that the product Dermolab NF cream meets the required validation criteria. The validation process was described and developed using the agar diffusion method, with Staphylococcus epidermidis ATCC 12228 as the test microorganism. The analytical parameters evaluated included specificity (selectivity), linearity, range, accuracy, and precision. Statistical evaluation of the experimental results, based on the established acceptance criteria, demonstrated that the analytical method is specific, linear (r² = 0.98 for the standard and r² = 0.97 for the sample, with a minimum specification of 0.95), precise (CV = 2.03% and 1.73%, with a specification of <5%; tolerance = 19.15% and 16.28%, corresponding to repeatability and intermediate precision, respectively, with a specification of <30%), and accurate (bias = 1.06%, with a specification of <3%; G_exp = 0.511 < G_tab = 0.871; T_exp = 0.765 < T_tab = 2.306) within the studied concentration range. Therefore, based on the studies performed, it is established that the analytical performance characteristics meet the requirements for the proposed analytical application, providing reliable results for verifying the quality specifications of the product.