Examinando por Autor "Helguera Carrasco, Alicia"

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    Interference due to turbidity (lipemia) in the determination of enzymes of clinical interest - Lima - 2023
    (Universidad Privada Norbert Wiener, 2024-06-30) Helguera Carrasco, Alicia; Saldaña Orejón, Italo Moisés
    Analytical interference is one of the main causes of clinically significant bias in the measurement of biological magnitudes. Lipemia-induced turbidity is one of the most common pre-analytical interferences in the clinical laboratory. The objective is to assess the effect of lipemia-induced turbidity in serum samples on the evaluation of the most common enzymes. Methods: A pre-experimental design was used with pre-test and post-test with a single group. From a pool of sera, seven aliquots were prepared to which increasing amounts of a lipid emulsion were added to mimic turbid samples, and one aliquot without the added interferent was used as a control. Results: The enzyme activity values for amylase, lipase, AST, and ALT were above the tolerability limits, while the bias in enzyme activities of FAL, GGT, CK, and LDH did not exceed these thresholds. Conclusion: Lipemia-induced turbidity significantly affected the enzymes amylase, lipase, AST, and ALT, while no significant effect was observed for the enzymes FAL, GGT, CK, and LDH.