Examinando por Autor "Meléndez Curasi, José Alejandro"

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Determinación de la hormona gonadotropina coriónica humana en manchas secas de sangre para la aplicación en la escena del delito - Distrito Fiscal de Ica 2020-2021
(Universidad Privada Norbert Wiener, 2023-04-20) Meléndez Curasi, José Alejandro; Montellanos Cabrera, Henry Sam
The human chorionic gonadotropin hormone (HCG) begins to be produced in large quantities after the implantation of the zygote, and a series of laboratory tests have been designed for clinical diagnosis; in the field of criminalistics there is a high casuistry of crimes against pregnant women, and the blood remains at the scenes where the events occurred; The objective of this study was to determine the permanence of the human chorionic gonadotropin hormone in dried blood spots of pregnant women, who remain at the scene of a crime, 22 venous blood samples were collected from pregnant women, from these samples Blood stains were produced on cotton cloth supports, the same ones that were left to dry in the environment for three months, from these stains the determination of the hormone was carried out using two methods, a qualitative immunochromatographic method and a quantitative ELISA method. It was obtained as a result that 100% of the blood spots were positive for the HCG hormone. Finally, it was concluded that HCG remains unchanged in dried blood stains from pregnant women; In addition, no differences were found in the determination of the hormone, between the qualitative method by immunochromatography and the quantitative method by ELISA, with both methods a sensitivity and specificity of 100% was found in the samples tested; it is recommended that the HCG dry spot test be implemented in the forensic field.