Examinando por Autor "Muñiz Mayta, Liseth"

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Acceso abierto
Actividad diurética del extracto hidroalcohólico de las hojas secas de la Persea americana Mill "palta fuerte"
(Universidad Privada Norbert Wiener, 2018-07-05) Bonifaz Monrroy, Nathali Dámariz; Muñiz Mayta, Liseth; Félix Véliz, Luis Miguel Visitación
The present study was conducted with the objective of determining the diuretic activity of the hydroalcoholic extract of dried leaves of Persea americana Mill. “Palta fuerte” in rats. The type of study was experimental and prospective, carried out in the natural products and animal facility laboratories of the Pharmacy and Biochemistry Department at Norbert Wiener University. The sample was collected in the province of Cañete, department of Lima. The hydroalcoholic extract was prepared for solubility testing, phytochemical analysis, and determination of diuretic activity using the Naik et al. method in rats divided into five groups of five each: Group I was the control, Group II received hydrochlorothiazide, and Groups III, IV, and V received doses of 100, 200, and 400 mg/kg of the extract. The percentage of urinary volumetric excretion, diuretic activity, and electrolyte quantification (Na⁺, K⁺, and Cl⁻) were calculated. It was determined that the extract is soluble in polar solvents and insoluble in non-polar solvents; the secondary metabolites present were tannins, saponins, phenols, alkaloids, and flavonoids. The percentages of urinary volumetric excretion were 28%, 30%, and 46% at doses of 100, 200, and 400 mg/kg, respectively, compared to hydrochlorothiazide which was 49%. The diuretic activity was 81%, 85%, and 95% at doses of 100, 200, and 400 mg/kg, respectively (p < 0.05); and significant differences were also observed with respect to electrolytes. In conclusion, it was demonstrated that the hydroalcoholic extract of the dried leaves of Persea americana Mill. “Palta fuerte” presented moderate diuretic activity.