Examinando por Autor "Ordoñez Lopez, Carmen Jenny"

Mostrando 1 - 4 de 4

Acceso abierto
Antibacterial effect of Trimix-MP paste and Fortrimax paste on Enterococcus faecalis strain: in vitro study, Lima 2017
(Universidad Privada Norbert Wiener, 2018-12-11) Zevallos Yanqui, Angélica; Ordoñez Lopez, Carmen Jenny
The objective of this research was to determine the antibacterial effect of Fortrimax paste in comparison with Trimix-MP paste on the Enterococcus Faecalis ATCC® 29212 strain. This study was developed in vitro at the microbiology laboratory of Universidad Privada Norbert Wiener, using the modified well diffusion method. The study population consisted of 160 Petri dishes containing Mueller Hinton agar, inoculated with Enterococcus faecalis, and distributed into 40 Petri dishes for each sample. A 6mm well was perforated in each plate, where the antibiotic paste was deposited, and incubated at 37ºC. The measurements of inhibition halos were taken at 24, 48 hours, and 7 days. The data were processed using the Mann-Whitney statistical test for comparisons. The Fortrimax paste showed a greater inhibitory effect, with an average of 36.5mm at 24 hours, 39.06mm at 48 hours, and 39.79mm at 7 days, showing an increasing trend in inhibition. The Trimix paste showed 30.38mm at 24 hours, 29.70mm at 48 hours, and 29.41mm at 7 days of inhibition. Finally, the positive control paste (Calcium Hydroxide) showed 13.15mm at 24 hours, 13.08mm at 48 hours, and 12.90mm at 7 days, demonstrating a lower inhibitory effect. The study concluded that Fortrimax paste had a better antibacterial effect and higher inhibition compared to Trimix paste and Calcium Hydroxide paste on the Enterococcus faecalis strain.
Acceso abierto
Degree of microbial contamination on the surface of a high-speed handpiece after an operating procedure at the dental clinic of the Private University Norbert Wiener, Lima 2019
(Universidad Privada Norbert Wiener, 2019-07-02) Gutiérrez Anicama, Fernando Adolfo; Ordoñez Lopez, Carmen Jenny
The general objective of this research work was to determine the degree of microbiological contamination on the surface of a high-speed handpiece after a surgical procedure in the treatment of patients attending the dental clinic of the Universidad Privada Norbert Wiener. For this purpose, 52 high-speed handpieces were evaluated, and swabs were taken from the handle and head of each handpiece. The samples were placed in various test tubes containing 1 ml of peptone water, which were then transported to the microbiology laboratory for inoculation onto petri dishes to assess the Colony Forming Units (CFU) present in the sample. The results revealed that the microbiological contamination on the head of the handpiece was 182.69 ± 187.47 CFU, while the handle showed 176.92 ± 189.55 CFU. Furthermore, 51.9% of the heads of high-speed handpieces showed microbial contamination, as did 50% of the handles. Additionally, 51.9% of the high-speed handpieces were contaminated with bacteria, while the remaining 48.1% showed no contamination from any microorganisms. The study concluded that the degree of microbiological contamination on the surface of the high-speed handpiece (both head and handle) following a surgical procedure in the dental clinic of the Universidad Privada Norbert Wiener in Lima, 2019, was high.
Acceso abierto
Effectiveness of xylol, orange oil and eucalyptus oil in dissolving gutta-percha: in vitro study
(Universidad Privada Norbert Wiener, 2019-02-25) Menacho Valdivieso, Johnny Ronal; Ordoñez Lopez, Carmen Jenny
The objective of this research was to determine the effectiveness of xylene, orange oil, and eucalyptus oil in dissolving gutta-percha. For this purpose, three solvent substances commonly used in dentistry (xylene, orange oil, and eucalyptus oil) and 60 gutta-percha discs, 8 millimeters in diameter and 2 millimeters in width, were used. The sample size was calculated using a sample calculation method, with 20 gutta-percha discs for each substance used. The initial weight of the discs was recorded, and then they were placed in Petri dishes containing the studied substances. The discs were submerged in these substances and were removed at 2 minutes and 5 minutes for immersion in distilled water to stop the solvent effect. The discs were weighed again, and the weight was recorded in the data collection sheet. The results showed that xylene dissolved 0.53 grams of gutta-percha after 2 minutes of exposure. Orange oil dissolved 0.44 grams, and eucalyptus oil dissolved 0.39 grams. After 5 minutes, xylene dissolved 1.15 grams of gutta-percha, while orange oil dissolved 0.94 grams, and eucalyptus oil dissolved 0.89 grams of gutta-percha. It was concluded that there was no statistically significant difference between the various gutta-percha solvents at 2 and 5 minutes of exposure.
Acceso abierto
Evaluation of the antibacterial capacity of two endodontic cements based on Calcium Hydroxide and Zinc Oxide against Enterococcus Faecalis strains. In vitro study.
(Universidad Privada Norbert Wiener, 2020-11-04) Gonzales Rojas, Heidy Aracely; Ordoñez Lopez, Carmen Jenny
The objective of this study was to determine the antibacterial capacity of two endodontic cements based on calcium hydroxide and zinc oxide against Enterococcus Faecalis strains. In this in vitro, experimental, longitudinal, and prospective research, the sample consisted of 40 specimens divided into two experimental groups and two control groups. The agar diffusion test was used to determine the antibacterial capacity. For this, the Enterococcus Faecalis ATCC 29212 strain was inoculated onto Mueller Hinton agar plates, and four equidistant perforations were made where the materials were placed. The antibacterial capacity was evaluated by measuring the inhibition halos at 2, 24, and 48 hours. It was found that chlorhexidine gluconate produced the largest bacterial growth inhibition halos, which were statistically significant (p<0.05). When comparing the endodontic cements, it was found that the calcium hydroxide-based endodontic cement produced the largest bacterial growth inhibition halos, which were statistically significant compared to the zinc oxide-based endodontic cement (p<0.05). Conclusion: The calcium hydroxide-based endodontic cement demonstrated better antibacterial capacity against Enterococcus Faecalis.