Examinando por Autor "Rojas León, Roberto Eugenio"

Mostrando 1 - 4 de 4

Acceso abierto
Differentiation of Candida albicans from Candida dubliniensis in tobacco agar, prepared with tobacco from three cigar brands
(Universidad Privada Norbert Wiener, 2018-09-28) Abanto Castillo, Johan Alexander; Valverde Ticlia, Fanny Noemí; Rojas León, Roberto Eugenio
The objective was to describe the differentiation of Candida albicans from Candida dubliniensis on tobacco agar, prepared with tobacco from three cigarette brands. This observational, cross-sectional, descriptive study was carried out with two strains of Candida albicans and Candida dubliniensis, isolated and confirmed by the Microbiology Service of the National Institute of Child Health - Breña. These strains were then plated on tobacco agar prepared with tobacco from three cigarette brands (Hamilton, Premier, and Pallmall) and incubated for 96 hours at 28°C in the microbiology service of the Huaycan hospital. Macroscopic and microscopic differences between Candida albicans and Candida dubliniensis were observed 72 hours after incubation, achieving the expected research objectives. This allowed for the description of the specific differences between Candida albicans and Candida dubliniensis through tobacco agar, giving a good specificity of approximately 100%, as described by some authors.
Acceso abierto
Egg Albumin Germ Tube Test Performance in Identifying Candida Albicans, 2020
(Universidad Privada Norbert Wiener, 2020-03-11) Arenas Pillco, Liliana Veronica; Rosas Paytan, Ruth Victoria; Rojas León, Roberto Eugenio
Candida albicans identification is performed with the germ tube test using human serum, which, despite being accessible, is not biosecure. Various researchers have conducted this test using egg albumin with satisfactory results but did not measure its performance. The aim of this study was to evaluate the performance of the germ tube test with egg albumin in identifying Candida albicans. This was a quantitative, observational, analytical, cross-sectional study conducted at the Norbert Wiener University Laboratory, following INS protocols. The germ tube test was performed using egg albumin from Gallus gallus domesticus, employing 118 Candida albicans strains and 12 non-Candida albicans strains. Sensitivity, specificity, false positives, false negatives, and efficiency were: 95.8%, 100%, 0%, 4.2%, and 96.2%, respectively. The Kappa index was 0.81, indicating high agreement with the reference method. Following SEIMC criteria, it was concluded that the test using egg albumin is acceptable for identifying Candida albicans and is an economical, accessible, and, above all, safe medium for laboratory personnel.
Acceso abierto
Susceptibility to Fluconazole and Voriconazole in strains of the genus Candida, isolated in vaginal secretions from patients of reproductive age treated in a Category I-3 Polyclinic, Lima.
(Universidad Privada Norbert Wiener, 2020-10-15) Chanco Lapa, Rocio; Vega Ordinola, Jean Pierre Andrés; Rojas León, Roberto Eugenio
Objective: To determine the susceptibility to fluconazole and voriconazole in strains of the genus Candida, isolated from vaginal secretion of reproductive-age patients treated at a Polyclinic category I-3, Lima.
Acceso abierto
Validation of the modified griess test for the detection of nitrite in urine from women of reproductive age with significant bacteriuria attended at San Bartolomé Hospital, November 2014 – January 2015
(Universidad Privada Norbert Wiener, 1905-07-07) Guillén Cabezas, Rosa Evelyn; Gutierrez Galindo, Luz Emperatriz; Rojas León, Roberto Eugenio
The objective of this study was to validate and determine the sensitivity, specificity, and predictive values of the modified Griess test for detecting nitrite in the urine of women of childbearing age with significant bacteriuria treated at the San Bartolomé Hospital from November 2014 to January 2015. Our research was descriptive, quantitative, and cross-sectional, as it is a primary validation study that resulted in numerical and descriptive specifications. Results showed 71.6% sensitivity, 99.4% specificity, 94.6% positive predictive value, and 96.3% negative predictive value. We concluded that the modified Griess test is not valid for detecting significant bacteriuria because it is a screening test that needs to have high sensitivity.