Examinando por Autor "Serván Meléndez, Janet"

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    Evaluación de la toxicidad aguda del extracto etanólico de las hojas de Minquartia guianensis Aubl “huacapú” en Rattus norvegicus Var "Holtzman"
    (Universidad Privada Norbert Wiener, 2023-11-09) Espinoza Rivera, Julia; Serván Meléndez, Janet; Chávez Flores, Juana Elvira
    Medicinal plants used as therapeutic alternatives contain phytoconstituent compounds that substitute for pharmaceuticals in the traditional medicine of Amazonian peoples, often applied empirically. Excessive administration of these plants may cause intoxication and pose health risks. Objective: To evaluate the acute toxicity of the ethanolic extract of Minquartia guianensis Aubl (“huacapú”) leaves in Rattus norvegicus var. “Holtzman.” Methodology: An analytical method was developed with a quantitative approach, applied type, and experimental design. The ethanolic extract obtained from huacapú leaves was studied in a sample of 30 rats. Phytochemical qualitative analysis was carried out through techniques of collection, maceration, extraction, and identification of secondary metabolites. For the acute toxicity study (limit dose 2000 mg/kg), 20 rats received a single oral dose of the ethanolic extract, and 10 rats received distilled water orally. The Irwin test was applied, and clinical, physiological, and behavioral manifestations were recorded. Rats were observed for 14 days, and on the 15th day, euthanasia was performed; intracardiac blood samples were collected for hematological analysis, and evisceration was carried out for anatomopathological examination. Results: No deaths were recorded, no significant hematological alterations were observed, and no anatomopathological lesions were found; the Irwin test results were normal. Conclusion: The ethanolic extract of Minquartia guianensis Aubl (“huacapú”) leaves, at a dose of 2000 mg/kg, did not present acute toxicity according to hematological analysis, anatomopathological findings, and the Irwin test. Further complementary research is suggested.