Examinando por Materia "Óvulo"

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Comparison of duck egg and chicken egg in the preparation of ogawa medium to decrease the average growth time of mycobacterium tuberculosis
(Universidad Privada Norbert Wiener, 2022-09-17) Guevara Perez, Lloy Italo; Rosas Paredes, Antino Rogelio; Benites Azabache, Juan Carlos
Tuberculosis, in any of its manifestations, is currently one of the most important public health issues, and the search for a rapid diagnostic method has been and continues to be one of the primary objectives in many parts of the world, especially in countries like ours, which have high incidence rates, and where the most affected population lacks the financial means to access existing rapid diagnostic methods. Objective: The aim of this study was to evaluate the Ogawa culture medium with duck egg for the diagnosis of Mycobacterium tuberculosis, comparing the growth time and growth characteristics of this medium at 15, 30, 45, and 60 days. Methodology: A descriptive, comparative study was conducted using 50 samples from asymptomatic respiratory patients and TB patients (++, +++). Patients with ongoing treatment, paucibacillary patients, treatment dropouts, and relapses were excluded, and the Ogawa culture method was used. Bacterial growth was assessed in the culture media at 15, 30, 45, and 60 days, as well as the average growth time using duck egg and chicken egg. Results: The average growth time of M. tuberculosis in the Ogawa medium with duck egg was 17.3 days, while with chicken egg it was 23.6 days, with a significant difference between both averages (p=0.000). The colony growth on duck egg was significantly higher than on chicken egg at 15 days (p=0.002), 30 days (p=0.001), and 45 days (p=0.025). No significant difference was found in colony growth at 60 days (p=0.215). Conclusion: The use of the Ogawa medium with duck egg is justified as an alternative for the presumptive diagnosis of Mycobacterium tuberculosis.