Examinando por Materia "Adhesins, Escherichia coli"

Mostrando 1 - 1 de 1

Acceso abierto
Phenotypic and molecular characterization of strains producing extended-spectrum beta-lactamases isolated from pediatric patients, 2018
(Universidad Privada Norbert Wiener, 2019-07-30) Ancajima Ballena, Maria Elena; Sotelo Alejos, Rocío Maribel; Benites Azabache, Juan Carlos
The resistance to beta-lactams in the pediatric population has not been previously evaluated in our community. This research aimed to characterize phenotypically and molecularly the extended-spectrum beta-lactamase (ESBL)-producing strains isolated from pediatric patients during the second semester of 2018. The study was prospective, cross-sectional, and descriptive, involving 30 pediatric patient samples. Data analysis was performed using the SPSS v21.0 statistical package, and tables and graphs were created in MS-Excel 2010. ESBL-producing strains were identified using antimicrobial susceptibility methods and CLSI breakpoint criteria, and the genes blaTEM, blaSHV, and blaCTX were characterized using conventional PCR. Most of the samples analyzed were urine (90%), with E. coli (76%) and K. pneumoniae (10%) being the most frequent bacterial agents. E. coli strains presented the blaCTX gene (87%), the blaTEM gene (83%), and the blaSHV gene (74%). Two strains (66.7%) of K. pneumoniae presented the blaCTX and blaTEM genes simultaneously, and two strains (66.7%) of P. mirabilis presented the blaCTX gene. The phenotypic and molecular characteristics of ESBL-producing strains isolated from pediatric patients indicated bacterial resistance, with the blaCTX gene being the most frequently found.