Examinando por Materia "Antibacterial effect"

Mostrando 1 - 5 de 5

Acceso abierto
Antibacterial effect of irrigating compounds using 5.25% sodium hypochlorite, 40% citric acid and 2% chlorhexidine gluconate against Enterococcus faecalis strain - in vitro - Lima, 2023
(Universidad Privada Norbert Wiener, 2023-12-04) Pomacaja Ponce, Yuri Evelin; Huamani Caquiamarca, Yuliana Esther
The objective of this study was to determine the antibacterial effect of irrigating compounds against the Enterococcus faecalis strain. The methodology employed was applied, with an analytical experimental design. A sample of 80 plates inoculated with the E. faecalis strain was used, which were treated with each of the irrigating compounds. The diameter of the inhibition zones was evaluated at three different times: 24, 48, and 72 hours, using the Duraffourd scale as the classification criterion. The results showed that sodium hypochlorite-based irrigating compounds at 5.25% had an average inhibition zone diameter of 11.44 ± 2.05 mm, classifying them as "sensitive." Citric acid-based compounds at 40% exhibited an average inhibition zone diameter of 24.41 ± 2.18 mm, classifying them as "highly sensitive." Furthermore, digluconate of chlorhexidine 2% based compounds presented an average inhibition zone diameter of 19.39 ± 1.18 mm, classifying them as "very sensitive." In conclusion, the evaluated irrigating compounds demonstrated an antibacterial effect against the E. faecalis strain.
Acceso abierto
Antibacterial effect of Stevia rebaudiana, Camellia sinensis and Origanum vulgare against Streptococcus mutans in vitro study, Lima - 2023
(Universidad Privada Norbert Wiener, 2024-06-04) Mamani Turpo, Luz Delia; Chilón Minaya, Lesly Johanna
The aim of this study was to determine the antibacterial effect of three plant-based compounds: Stevia rebaudiana, Camellia sinensis, and Origanum vulgare, against Streptococcus mutans. An in vitro experimental design methodology was used, with an analytical method and applied type. Treatments were applied to Petri dishes with Streptococcus mutans strains, using 10 plates for each treatment and two control groups. The results revealed that, of the three plant-based compounds tested, two showed antibacterial effects against Streptococcus mutans. For Origanum vulgare, inhibition halos of 32.09 mm were obtained at 24 hours and 31.80 mm at 48 hours. For Camellia sinensis, inhibition halos were 25.06 mm at 24 hours and 24.74 mm at 48 hours. However, Stevia rebaudiana did not show an antibacterial effect as its inhibition halos were 0 mm. Therefore, it was concluded that the antibacterial compounds that did show antibacterial effects did not show significant differences compared to the positive control used, 0.12% Chlorhexidine Gluconate (p>0.05).
Acceso abierto
Comparison of the antibacterial effectiveness of irrigants based on 0.12% chlorhexidine and ozonized sunflower oil on porphyromonas gingivalis strains - in vitro study, Lima - 2023
(Universidad Privada Norbert Wiener, 2023-06-08) Blas Huaroc, Esteban Abraham; Huayllas Paredes, Betzabé
The objective was to determine the antibacterial effectiveness of irrigants based on 0.12% Chlorhexidine and ozonized sunflower oil against Porphyromonas gingivalis strains. This was an applied research study with an analytical method, quantitative focus, and an in-vitro experimental design, where microorganisms were cultured on 40 Petri dishes containing agar. The results showed significant antibacterial effects of both 0.12% Chlorhexidine and ozonized sunflower oil, according to the Duraffourd Scale. Chlorhexidine 0.12% showed average inhibition zones of 17.58 mm at 24 hours, 17.56 mm at 48 hours, and 17.55 mm at 72 hours. On the other hand, ozonized sunflower oil showed inhibition zones of 36.5 mm at 24 hours, 36.49 mm at 48 hours, and 36.47 mm at 72 hours. It was concluded that ozonized sunflower oil had a better antibacterial effect than 0.12% Chlorhexidine against Porphyromonas gingivalis strains.
Acceso abierto
In vitro antibacterial effect of chamomile (Matricaria chamomilla l) in essential oil and infusion on Streptococcus mutans
(Universidad Privada Norbert Wiener, 2022-12-07) Gomero Saenz, Nelson Ricardo; Vilchez Bellido, Dina
Chamomile (Matricaria chamomilla L) has anti-inflammatory, antioxidant, analgesic, and bacteriostatic properties that could help control oral microorganisms such as Streptococcus mutans. The objective of this research was to determine the in vitro antibacterial effect of chamomile, in essential oil and infusion, on Streptococcus mutans. The experimental study used the Kirby Bauer technique, which involves depositing antibiotic-impregnated discs containing chamomile essential oil (35%), chamomile infusion (20%), chlorhexidine 0.12% (positive control group), and distilled water (negative control group) on blood agar plates inoculated with Streptococcus mutans ATCC 25175 (n=44 Petri dishes, 11 for each tested substance), evaluating the formation of an inhibition zone. Descriptive and inferential statistics were performed (p ≤ 0.05 considered significant). The results showed that chamomile essential oil exhibited an antibacterial effect on Streptococcus mutans, unlike chamomile infusion, which showed no effect. The mean inhibition zones were 7.15 mm for chamomile essential oil (Matricaria chamomilla L) and 25.09 mm for chlorhexidine, after 48 hours of incubation. It was concluded that chamomile essential oil exhibited an antibacterial effect on Streptococcus mutans.
Acceso abierto
In vitro evaluation of the antibacterial activity of ionomers of encapsulated glass and powder-liquid for ART restoration on cultures of streptococcus mutans and lactobacillus acidophilus, Lima 2021.
(Universidad Privada Norbert Wiener, 2022-11-30) Vilca Manrique, Alberto Gustavo; Céspedes Porras, Jacqueline
This study evaluated, determined, and compared the antibacterial effect of glass ionomers for ART restoration, both encapsulated (Riva Self Cure® and Equia Forte®) and powder-liquid (Fuji IX® and Ketac Molar Easymix®), against cultures of Streptococcus mutans (ATC 35668) and Lactobacillus acidophilus (ATC 4356). The methodology was experimental, quasi-experimental, in vitro, conducted in the Herrera S.A.C. Clinical Laboratory and medical offices. Forty Petri dishes were grouped into two groups according to the bacteria, inoculating the encapsulated and powder-liquid ionomers with bacterial strains using the diffusion technique. The data was collected by measuring the inhibition halos with a vernier and processed statistically. Results showed that the inhibition halos on Streptococcus mutans (ATC 35668) cultures formed by encapsulated glass ionomers were 9.4 + 2.11 mm for Riva Self Cure® and 9.0 + 2.16 mm for Equia Forte®. For powder-liquid ionomers, the halos were 8.9 + 1.66 mm for Ketac Molar Easymix® and 9.6 + 1.83 mm for Fuji IX®. Likewise, the mean inhibition halos on Lactobacillus acidophilus (ATC 4356) cultures obtained by the encapsulated glass ionomers were 9.3 + 1.76 mm for Riva Self Cure® and 9.6 + 2.5 mm for Equia Forte®. The halos obtained by powder-liquid ionomers were 8.9 + 1.1 mm for Ketac Molar Easymix® and 8.7 + 1.63 mm for Fuji IX®. Conclusion: There is in vitro antibacterial activity of glass ionomers (Riva Self Cure® and Equia Forte®) and powder-liquid (Fuji IX® and Ketac Molar Easymix®) for ART restoration against cultures of Streptococcus mutans (ATC 35668) and Lactobacillus acidophilus (ATC 4356).