Examinando por Materia "Bacterial contamination"

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(Universidad Privada Norbert Wiener, 2015) Trauco Villavicencio, Sandra Lizzeth; Galvez Ramirez, Carlos Michell
The objective of this study was to determine the efficacy of 0.12% Chlorhexidine and 0.1% and 0.2% Sodium Hypochlorite for controlling the most prevalent bacterial contamination on toothbrushes used by 7-year-old schoolchildren at the Parroquial Nuestra Señora de Montserrat Educational Institution. To this end, the toothbrushes used by the schoolchildren for a period of 47 days were collected in sterile urocultivation containers containing 5 ml of sterile TSB broth and incubated at 35°C for 18 hours. Then, 1 μl (0.001 ml) of the broth was streaked and diluted on MacConkey agar plates, which were incubated at 35°C for 18 hours. The reading was based on colonial characteristics, followed by microorganism identification. The antibacterial substances in Mueller Hinton plates were incubated for 24 hours at 35°C, after which the inhibition zones were measured with a Vernier caliper or ruler. The results showed that the most prevalent microorganisms on the toothbrushes used by the schoolchildren were Pseudomonas spp. (28.3%) and Pseudomonas aeruginosa (6.5%) on non-contaminated plates, while on contaminated plates, Pseudomonas spp. accounted for 65% and Pseudomonas aeruginosa for 15%. The antibacterial efficacy identified that 0.12% Chlorhexidine had a greater inhibitory effect for controlling bacterial contamination found on the toothbrushes compared to 0.1% and 0.2% Sodium Hypochlorite. It was concluded that the most prevalent bacteria were more resistant to the inhibitory effect of 0.1% and 0.2% Sodium Hypochlorite.