Examinando por Materia "Biopelículas"

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  • Miniatura
    ÍtemAcceso abierto
    Association between biofilm formation and the production of extended-spectrum beta-lactamases in Escherichia coli isolated from urine culture at Hipólito Unanue National Hospital, January to June 2018
    (Universidad Privada Norbert Wiener, 2019-01-23) Hurtado Quispe, Luz Melina; Casablanca Alvarado, Juana; Champi Merino, Roky Govanni
    The objective was to determine the association between biofilm formation and the production of extended-spectrum beta-lactamases (ESBL) in Escherichia coli isolated from urine cultures at the Hipólito Unanue National Hospital from January to June 2018." A quantitative, observational, and cross-sectional study was conducted. The Jarlier method was used to demonstrate ESBL production, and the Tissue Culture Plate (TCP) method was used to assess biofilm formation. The variables were described using absolute and percentage frequencies, and the association between the variables was determined using the Chi-square test. Out of 190 Escherichia coli strains isolated from urine cultures, 48.9% produced ESBL and 73.2% were biofilm producers, with 58.3% weak, 30.2% moderate, and 11.5% strong biofilm formation.
  • Miniatura
    ÍtemAcceso abierto
    Relationship between biofilm formation and oxacillin resistance in staphylococcus aureus isolated from blood cultures. Hipólito Unanue National Hospital, July 2016 – June 2018
    (Universidad Privada Norbert Wiener, 2020-01-18) Zavala Castillo, Rosa Iris; Yalle Cotrina, Oliver Francisco; Champi Merino, Roky Govanni
    The objective of this research was to determine the relationship between biofilm formation and oxacillin resistance in Staphylococcus aureus isolated from blood cultures at the Hipólito Unanue National Hospital. The sample consisted of 71 Staphylococcus aureus strains isolated from blood cultures. The study was quantitative, observational, and correlational. Statistical analysis was done using SPSS version 22 and WHONET 5.6, employing the Chi-square test for variable association. The frequency of Staphylococcus aureus resistance to oxacillin was determined using the clinical laboratory standard (CLSI) cutoff values, finding a 63.4% resistance rate. The tissue culture plate method (PCT) was used for biofilm determination, with 55 strains (77.5%) exhibiting biofilm formation capacity, while 16 strains (22.5%) were non-biofilm producers. Biofilm-producing strains were categorized as strong (49.3%), moderate (18.3%), and weak (9.9%), while 22.5% did not exhibit biofilm formation. The study concluded that no significant relationship exists between oxacillin resistance and biofilm formation in Staphylococcus aureus strains isolated from blood cultures of hospitalized patients at the Hipólito Unanue National Hospital (July 2016 - June 2018).