Examinando por Materia "Campylobacter"

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    Correlation Between Interrupted Gram Staining and Culture with Filters Using the Klebsiella Technique in the Presumptive Identification of Campylobacter sp. in Stool Samples from Children at a National Hospital in Lima, July 2015 to June 2016
    (Universidad Privada Norbert Wiener, 2017-12-19) Ocampo Ramirez, Lesslie Fiorella; Chuquiray Valverde, Nancy Norma
    Currently, Campylobacter sp. is a bacterial agent of foodborne transmission and a zoonosis of worldwide distribution. Campylobacter enteritis ranges from watery diarrhea, without blood, to dysenteric syndrome with abdominal pain and fever, typically self-limiting and indistinguishable from gastrointestinal infections caused by other enteropathogens. The objective of this study was to determine the correlation between interrupted Gram staining and filtration culture techniques with Klebsiella in the presumptive identification of Campylobacter sp. in stool samples from children at a National Hospital in Lima, from July 2015 to June 2016. The study is non-experimental, with a cross-sectional, correlational, descriptive design. A total of 414 stool samples from pediatric patients with positive inflammatory reaction were evaluated during July 2015 to June 2016 at the Alberto Sabogal Sologuren Hospital laboratory, with 43 positive cases (10.4%) for Campylobacter sp. The interrupted Gram stain showed 46.5% sensitivity and 99.2% specificity. Although the sensitivity of interrupted Gram staining was low, the culture method can increase sensitivity, making it a low-cost strategy for diagnosing Campylobacter sp.-caused diarrheal infections.