Examinando por Materia "Contact Inhibition"

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    Comparison of two methods for the detection of residual antimicrobial activity in urine cultures processed at the National Teaching Hospital Madre Niño San Bartolomé
    (Universidad Privada Norbert Wiener, 1905-07-07) Rodriguez Vera, Claudia Liliana; Olivo Lopez, Jose Maria
    Objective: To determine which method, paper diffusion or direct inoculation, detects residual antimicrobial activity more frequently in urine cultures processed at the National Mother-Child Teaching Hospital in San Bartolomé. Materials and Methods: A descriptive, cross-sectional study was conducted from January to March 2014, processing 2024 urine samples from outpatients and hospitalized patients. For both methods to detect residual antimicrobial activity, the samples were inoculated on Muller Hinton agar plates with the Bacillus subtilis ATCC 6633 sensor strain at a 0.5 McFarland scale. In the paper diffusion method, 10 µL of urine was applied on a disk, while in the direct inoculation method, 1 µL of urine was directly inoculated on the agar plate with a sterile loop. Both plates were incubated at 37°C for 24 hours. The presence of an inhibition zone around the disk or direct inoculation point was considered as the presence of residual antimicrobial activity. The absence of an inhibition zone was considered as the absence of residual antimicrobial activity. Results: Residual antimicrobial activity was detected in 9.7% (196/2024) of the urine samples using the paper diffusion method, compared to 6.4% (129/2024) using the direct inoculation method. Moreover, 67 (3.3%) urine samples showed residual antimicrobial activity detected only by the paper diffusion method. In total, 1828 (90.3%) samples showed no residual antimicrobial activity with either method, and 129 (6.4%) showed residual antimicrobial activity. Conclusions: The paper diffusion method has a higher detection rate for residual antimicrobial activity in urine samples processed at the National Mother-Child Teaching Hospital in San Bartolomé.