Examinando por Materia "Diagnostic Imaging"

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Acceso abierto
Validation of the extract from the Exocarp of Renealmia Alpinia (Kumpia) as a tissue nuclear staining agent
(Universidad Privada Norbert Wiener, 1905-07-06) Santa Cruz Sánchez, Óscar; García Vásquez, Carlos Hugo
From the extract of the exocarp of Renealmia alpinia (kumpia), originating from the Amazon of Peru, through extraction by crushing and subsequent filtration, a 100% pure and natural dye with a purple hue was obtained. This dye underwent various tests of tissue nuclear staining, controlled by time (5, 10, and 15 minutes) and pH measurement, with alcohol at 70° as a mordant. Therefore, the objective of this study was to validate this dye (kumpia) as a tissue nuclear dye. An experimental study was conducted using surgical specimens from the Alberto Sabogal Sologuren Hospital. Tissue samples included liver, intestine, and lymph nodes. A total of 102 slides were obtained (34 for each tissue), of which 51 were stained with kumpia-eosin and 51 with hematoxylin-eosin, taken as the gold standard. Three evaluation criteria were considered in the microscopic assessment: histological image for both kumpia and hematoxylin staining, with results of 88.24% (good) and 11.76% (fair); nuclear staining for kumpia showed 94.12% (good) and 5.88% (fair); and 100% (good) for hematoxylin. For the nuclear-cytoplasm differentiation, both stains showed 100% (good). The conclusion is that this dye is good and effective, offering an easy-to-obtain alternative for tissue nuclear staining, and its cost is very affordable.