Examinando por Materia "Diuresis"

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Acceso abierto
Actividad diurética del extracto hidroalcohólico de las hojas secas de Baccharis genistelloides (Lam.) pers. (Carqueja) en ratas albinas de la cepa Holtzman
(Universidad Privada Norbert Wiener, 2021-05-20) López Dávila, Zaid Hebert; Chávez Flores, Juana Elvira
Medicinal plants with diuretic activity currently occupy a place as an alternative therapy to pharmaceutical drugs. Objective: To determine the diuretic activity of the hydroalcoholic extract from the dry leaves of Baccharis genistelloides (Lam.) Pers. (Carqueja) in Holtzman strain albino rats. Materials and method: 50 female rats weighing 230 to 280 g were used, employing the modified Naik et al. model. The rats were pre-hydrated with 0.9% sodium chloride (NaCl) at 50 mL/kg and distributed into five groups: Control Group (0.9% NaCl 50 mL/kg), Standard Group (furosemide 40 mg/kg), Group I (extract 200 mg/kg), Group II (extract 400 mg/kg), and Group III (extract 600 mg/kg). Urine was collected after 6 hours, followed by electrolyte dosage and the calculation of the percentage of urinary volumetric excretion (%EVU) and the percentage of diuretic activity (%AD). Results: At the sixth hour, the highest average urine volume was observed in Group III (8.63 mL). This group also showed 55.7% EVU. Regarding %AD, Group III presented the highest activity at 113.1%, which was superior to the control group. For electrolyte dosage, the observed differences in K+ and Na+ were not significant, meaning it cannot be asserted that the extract significantly alters these electrolytes, while the Cl- values of the extract groups were comparable to the control group. Conclusions: The hydroalcoholic extract from the dry leaves of Baccharis genistelloides (Lam.) Pers. (Carqueja) presented diuretic activity at all three concentrations used.
Acceso abierto
Actividad diurética del extracto hidroalcohólico de las hojas secas de la Persea americana Mill "palta fuerte"
(Universidad Privada Norbert Wiener, 2018-07-05) Bonifaz Monrroy, Nathali Dámariz; Muñiz Mayta, Liseth; Félix Véliz, Luis Miguel Visitación
The present study was conducted with the objective of determining the diuretic activity of the hydroalcoholic extract of dried leaves of Persea americana Mill. “Palta fuerte” in rats. The type of study was experimental and prospective, carried out in the natural products and animal facility laboratories of the Pharmacy and Biochemistry Department at Norbert Wiener University. The sample was collected in the province of Cañete, department of Lima. The hydroalcoholic extract was prepared for solubility testing, phytochemical analysis, and determination of diuretic activity using the Naik et al. method in rats divided into five groups of five each: Group I was the control, Group II received hydrochlorothiazide, and Groups III, IV, and V received doses of 100, 200, and 400 mg/kg of the extract. The percentage of urinary volumetric excretion, diuretic activity, and electrolyte quantification (Na⁺, K⁺, and Cl⁻) were calculated. It was determined that the extract is soluble in polar solvents and insoluble in non-polar solvents; the secondary metabolites present were tannins, saponins, phenols, alkaloids, and flavonoids. The percentages of urinary volumetric excretion were 28%, 30%, and 46% at doses of 100, 200, and 400 mg/kg, respectively, compared to hydrochlorothiazide which was 49%. The diuretic activity was 81%, 85%, and 95% at doses of 100, 200, and 400 mg/kg, respectively (p < 0.05); and significant differences were also observed with respect to electrolytes. In conclusion, it was demonstrated that the hydroalcoholic extract of the dried leaves of Persea americana Mill. “Palta fuerte” presented moderate diuretic activity.