Examinando por Materia "Edetic Acid"

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    Carbapenemases in Pseudomonas aeruginosa in Isolates from Hospitalized Patients of a National Hospital in San Juan de Miraflores, January to June 2017, Lima
    (Universidad Privada Norbert Wiener, 1905-07-09) Carranza Vásquez, Sherley Merlid; Vasquez Quispe, Walter Ivan; Olivo Lopez, Jose Maria
    Pseudomonas aeruginosa is one of the most common pathogens causing infections in hospitalized patients and produces various resistance mechanisms, among which carbapenemases that hydrolyze all beta-lactams are significant. The objective of our study was to determine the types of carbapenemases found in Pseudomonas aeruginosa isolates from hospitalized patients at a national hospital in San Juan de Miraflores, Lima in 2017. A basic, descriptive, prospective, and cross-sectional study was conducted, including 150 non-repeated Pseudomonas aeruginosa isolates from hospitalized patients, with sensitivity tests performed by disk diffusion according to CLSI 2017. Of these, 80 strains were suspected of producing carbapenemases. The phenotypic test for carbapenemase detection used the disk approximation method with substrates (imipenem and meropenem) and inhibitors (EDTA and ABP) according to the INEI-ANLIS Dr. C. Malbrán recommended algorithm. Results showed 3 (4%) strains positive for carbapenemases. We conclude that the type of carbapenemase found in Pseudomonas aeruginosa was 4%, corresponding to the detection of metallo-beta-lactamases, indicated by the synergistic effect between imipenem and EDTA. No serine carbapenemases were detected.