Examinando por Materia "Efecto antibacteriano"
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Ítem Acceso abierto Antibacterial effect of irrigating compounds using 5.25% sodium hypochlorite, 40% citric acid and 2% chlorhexidine gluconate against Enterococcus faecalis strain - in vitro - Lima, 2023(Universidad Privada Norbert Wiener, 2023-12-04) Pomacaja Ponce, Yuri Evelin; Huamani Caquiamarca, Yuliana EstherThe objective of this study was to determine the antibacterial effect of irrigating compounds against the Enterococcus faecalis strain. The methodology employed was applied, with an analytical experimental design. A sample of 80 plates inoculated with the E. faecalis strain was used, which were treated with each of the irrigating compounds. The diameter of the inhibition zones was evaluated at three different times: 24, 48, and 72 hours, using the Duraffourd scale as the classification criterion. The results showed that sodium hypochlorite-based irrigating compounds at 5.25% had an average inhibition zone diameter of 11.44 ± 2.05 mm, classifying them as "sensitive." Citric acid-based compounds at 40% exhibited an average inhibition zone diameter of 24.41 ± 2.18 mm, classifying them as "highly sensitive." Furthermore, digluconate of chlorhexidine 2% based compounds presented an average inhibition zone diameter of 19.39 ± 1.18 mm, classifying them as "very sensitive." In conclusion, the evaluated irrigating compounds demonstrated an antibacterial effect against the E. faecalis strain.Ítem Acceso abierto Antibacterial effect of Stevia rebaudiana, Camellia sinensis and Origanum vulgare against Streptococcus mutans in vitro study, Lima - 2023(Universidad Privada Norbert Wiener, 2024-06-04) Mamani Turpo, Luz Delia; Chilón Minaya, Lesly JohannaThe aim of this study was to determine the antibacterial effect of three plant-based compounds: Stevia rebaudiana, Camellia sinensis, and Origanum vulgare, against Streptococcus mutans. An in vitro experimental design methodology was used, with an analytical method and applied type. Treatments were applied to Petri dishes with Streptococcus mutans strains, using 10 plates for each treatment and two control groups. The results revealed that, of the three plant-based compounds tested, two showed antibacterial effects against Streptococcus mutans. For Origanum vulgare, inhibition halos of 32.09 mm were obtained at 24 hours and 31.80 mm at 48 hours. For Camellia sinensis, inhibition halos were 25.06 mm at 24 hours and 24.74 mm at 48 hours. However, Stevia rebaudiana did not show an antibacterial effect as its inhibition halos were 0 mm. Therefore, it was concluded that the antibacterial compounds that did show antibacterial effects did not show significant differences compared to the positive control used, 0.12% Chlorhexidine Gluconate (p>0.05).Ítem Acceso abierto Antibacterial effect of the hydroalcoholic extract of erythroxylum coca lam. (coca) compared to chlorhexidine gluconate 0.12% versus strains of streptococcus mutans. In vitro study. Lima 2021(Universidad Privada Norbert Wiener, 2021-12-04) Macedo Heredia, Raúl Enrique; Granados Carrera, Julio CesarObjetivo: Esta investigación tuvo como objetivo evaluar el efecto antibacteriano del extracto hidroalcohólico de Erythroxylum coca Lam. (coca) comparado con gluconato de clorhexidina al 0,12% contra cepas de Streptococcus mutans in vitro. Metodología: Se realizó un estudio cuantitativo, aplicado, cuasi experimental. La variable principal estudiada fue el agente antibacteriano, comparándose concentraciones de 50%, 75% y 100% del extracto hidroalcohólico de Erythroxylum coca Lam. (coca) con gluconato de clorhexidina al 0,12%. Se desarrollaron cultivos bacterianos de Streptococcus mutans utilizando una muestra de 70 placas. Resultados: El extracto hidroalcohólico de Erythroxylum coca Lam. (coca) mostró efectos antibacterianos contra Streptococcus mutans en las tres concentraciones a las 24 y 48 horas. El mayor halo de inhibición media se observó con una concentración del 50% a las 48 horas (31,01 mm), mientras que el menor se registró con clorhexidina al 0,12% a las 24 horas (10 mm). Conclusión: Existe una diferencia significativa en el efecto antibacteriano del extracto hidroalcohólico de Erythroxylum coca Lam. (coca) en comparación con el gluconato de clorhexidina al 0,12% frente a cepas de Streptococcus mutans in vitro.Ítem Acceso abierto Comparison of the antibacterial effectiveness of irrigants based on 0.12% chlorhexidine and ozonized sunflower oil on porphyromonas gingivalis strains - in vitro study, Lima - 2023(Universidad Privada Norbert Wiener, 2023-06-08) Blas Huaroc, Esteban Abraham; Huayllas Paredes, BetzabéThe objective was to determine the antibacterial effectiveness of irrigants based on 0.12% Chlorhexidine and ozonized sunflower oil against Porphyromonas gingivalis strains. This was an applied research study with an analytical method, quantitative focus, and an in-vitro experimental design, where microorganisms were cultured on 40 Petri dishes containing agar. The results showed significant antibacterial effects of both 0.12% Chlorhexidine and ozonized sunflower oil, according to the Duraffourd Scale. Chlorhexidine 0.12% showed average inhibition zones of 17.58 mm at 24 hours, 17.56 mm at 48 hours, and 17.55 mm at 72 hours. On the other hand, ozonized sunflower oil showed inhibition zones of 36.5 mm at 24 hours, 36.49 mm at 48 hours, and 36.47 mm at 72 hours. It was concluded that ozonized sunflower oil had a better antibacterial effect than 0.12% Chlorhexidine against Porphyromonas gingivalis strains.Publicación Acceso abierto Efecto antibacteriano del extracto etanólico del cáliz de Physalis peruviana (Aguaymanto) frente cepas de referencia, Lima Metropolitana, 2023(Universidad Norbert Wiener, 2024-05-04) Delgado Cubas, Luz Mery; Trujillo paredes, Edith; Collanque Pinto, Jesús DanielLa resistencia a los antibióticos es alarmante a nivel mundial, siendo Streptococcus mutans, Porphyromonas gingivalis y Staphylococcus aureus los principales implicados en este fenómeno. debido a lo mencionado que el presente trabajo tiene como objetivo: Determinar el efecto antibacteriano del extracto etanólico del cáliz de Physalis peruviana (aguaymanto) frente cepas de referencia, en Lima, 2023. Metodología: Se empleó el método hipotético-deductivo con un enfoque cuantitativo, se clasificó como aplicada, con un diseño de estudio experimental de corte transversal, de nivel explicativo; población frutos de Physalis peruviana y Streptococcus mutans, Porphyromonas gingivalis y Staphylococcus aureus, muestra fue 10 kilos de cáliz de Physalis peruviana, 15 placas petri el cual fueron distribuidas de 5 placas por cada cepa biológica y muestreo no probabilístico por intención. El procedimiento fitoquímico fue el análisis cualitativo preliminar y la parte biológica la difusión en agar en pozos. Resultados: Los promedios del 25%, 50% y 75% frente a las cepas de referencia fueron inferiores a los obtenidos por el control positivo (clorhexidina y ciprofloxacino). Así mismo, (p<0.000) entre los grupos experimentales y (DMSO). Conclusión: El extracto etanólico del cáliz de Physalis peruviana (aguaymanto) presentó actividad antibacteriana en todas las concentraciones frente a las cepas de referencia, en Lima, 2023, excepto en la concentración al 25%, la cual tuvo una media de 7,2160 mm frente la cepa Streptococcus mutans ATCC 25175.Publicación Acceso abierto Efecto antibacteriano del extracto metanólico de las hojas de Nasturtium officinale “berro” sobre cepas de Pseudomona aeruginosa, Escherichia coli y Staphylococcus aureus(Universidad Norbert Wiener, 2024-02-26) Broncano Giraldo, Anali Milagros; Pares Quispe, Lizeth Cristina; Collanque Pinto, Jesús DanielEl presente trabajo tiene como objetivo: Determinar el efecto antibacteriano del extracto metanólico de las hojas de Nasturtium officinale “berro” sobre cepas de Pseudomona aeruginosa ATCC 27853, Escherichia coli ATCC 10536 y Staphylococcus aureus ATCC 25923. Métodos: el método que se utilizo fue disco difusión de Kirby-Bauer, para evaluar el efecto antibacteriano se utilizaron 72 placas con agar Mueller Hinton, divididos en 4 grupos para las diferentes concentraciones. Resultados: El extracto metanólico a base de las hojas de Nasturtium Officinale “berro”, presenta efecto antibacteriano sobre la cepa de Pseudomona aeruginosa ATCC27853 en diferentes concentraciones a compasión de Escherichia coli ATCC 10536 y Staphylococcus aureus ATCC 25923 las cuales no presentaron efecto antibacteriano en ninguna concentración, el mayor efecto antibacteriano se dio en la concentración 100 mg/ml a diferencia de las demás concentraciones. Conclusión: Se demostró el efecto antibacteriano del extracto metanólico de las hojas de Nasturtium officinale “berro” sobre cepas de Pseudomona aeruginosa ATCC 27853.Ítem Acceso abierto In vitro antibacterial effect of chamomile (Matricaria chamomilla l) in essential oil and infusion on Streptococcus mutans(Universidad Privada Norbert Wiener, 2022-12-07) Gomero Saenz, Nelson Ricardo; Vilchez Bellido, DinaChamomile (Matricaria chamomilla L) has anti-inflammatory, antioxidant, analgesic, and bacteriostatic properties that could help control oral microorganisms such as Streptococcus mutans. The objective of this research was to determine the in vitro antibacterial effect of chamomile, in essential oil and infusion, on Streptococcus mutans. The experimental study used the Kirby Bauer technique, which involves depositing antibiotic-impregnated discs containing chamomile essential oil (35%), chamomile infusion (20%), chlorhexidine 0.12% (positive control group), and distilled water (negative control group) on blood agar plates inoculated with Streptococcus mutans ATCC 25175 (n=44 Petri dishes, 11 for each tested substance), evaluating the formation of an inhibition zone. Descriptive and inferential statistics were performed (p ≤ 0.05 considered significant). The results showed that chamomile essential oil exhibited an antibacterial effect on Streptococcus mutans, unlike chamomile infusion, which showed no effect. The mean inhibition zones were 7.15 mm for chamomile essential oil (Matricaria chamomilla L) and 25.09 mm for chlorhexidine, after 48 hours of incubation. It was concluded that chamomile essential oil exhibited an antibacterial effect on Streptococcus mutans.Ítem Acceso abierto In vitro evaluation of the antibacterial activity of ionomers of encapsulated glass and powder-liquid for ART restoration on cultures of streptococcus mutans and lactobacillus acidophilus, Lima 2021.(Universidad Privada Norbert Wiener, 2022-11-30) Vilca Manrique, Alberto Gustavo; Céspedes Porras, JacquelineThis study evaluated, determined, and compared the antibacterial effect of glass ionomers for ART restoration, both encapsulated (Riva Self Cure® and Equia Forte®) and powder-liquid (Fuji IX® and Ketac Molar Easymix®), against cultures of Streptococcus mutans (ATC 35668) and Lactobacillus acidophilus (ATC 4356). The methodology was experimental, quasi-experimental, in vitro, conducted in the Herrera S.A.C. Clinical Laboratory and medical offices. Forty Petri dishes were grouped into two groups according to the bacteria, inoculating the encapsulated and powder-liquid ionomers with bacterial strains using the diffusion technique. The data was collected by measuring the inhibition halos with a vernier and processed statistically. Results showed that the inhibition halos on Streptococcus mutans (ATC 35668) cultures formed by encapsulated glass ionomers were 9.4 + 2.11 mm for Riva Self Cure® and 9.0 + 2.16 mm for Equia Forte®. For powder-liquid ionomers, the halos were 8.9 + 1.66 mm for Ketac Molar Easymix® and 9.6 + 1.83 mm for Fuji IX®. Likewise, the mean inhibition halos on Lactobacillus acidophilus (ATC 4356) cultures obtained by the encapsulated glass ionomers were 9.3 + 1.76 mm for Riva Self Cure® and 9.6 + 2.5 mm for Equia Forte®. The halos obtained by powder-liquid ionomers were 8.9 + 1.1 mm for Ketac Molar Easymix® and 8.7 + 1.63 mm for Fuji IX®. Conclusion: There is in vitro antibacterial activity of glass ionomers (Riva Self Cure® and Equia Forte®) and powder-liquid (Fuji IX® and Ketac Molar Easymix®) for ART restoration against cultures of Streptococcus mutans (ATC 35668) and Lactobacillus acidophilus (ATC 4356).
