Examinando por Materia "Endodontic sealer"
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Ítem Acceso abierto effect of plant extracts mixed with an endodontic sealant on the growth of oral pathogens in the root canal an in vitro study(Universidad Privada Norbert Wiener, 2023-11-18) Diaz León, Marlon Omar; Guevara Sotomayor, Juan CésarThis research aimed to evaluate the effect of plant extracts (thyme, copaiba, and annatto) mixed with an endodontic sealer on the growth of oral pathogens in the root canal, such as Enterococcus faecalis and Streptococcus mutans. An in vitro experimental study with an applied analytical methodology was designed for this purpose. A total sample of 80 Petri dishes was divided into two main groups of 40 each for each microorganism, and further subdivided for each treatment and its control group. The results showed that all three plant extracts mixed with a zinc-based endodontic sealer had an inhibitory effect on the growth of the oral pathogens Enterococcus faecalis and Streptococcus mutans. The inhibition zones around the microorganisms were greater than zero in both the first evaluation at 24 hours and at 48 hours. Additionally, the highest effect was observed in the endodontic sealer added with thyme, showing a greater effect than the pure endodontic sealer for Enterococcus faecalis with 12.75 ± 1.64mm and 12.63 ± 1.65mm for the 24 and 48-hour evaluations, and for Streptococcus mutans, 19.59 ± 2.07mm and 19.47 ± 2.06mm for the 24 and 48-hour evaluations, respectively. Therefore, it was concluded that the evaluated plant extracts mixed with endodontic sealer do present an antibacterial effect.Ítem Acceso abierto In vitro evaluation of the antibacterial capacity of sealants endodontics based on epoxy resin and calcium hydroxide versus Enterococcus faecalis(Universidad Privada Norbert Wiener, 2023-09-07) Alvarez Vilca, Roel; Jaime Okumura, RobertoThe objective of this research was to determine, through an in vitro study, the antibacterial capacity of an epoxy resin and calcium hydroxide-based endodontic sealer against Enterococcus Faecalis. Materials and Methods: The antibacterial capacity was evaluated using the disc diffusion test. For this, 14 Petri dishes were prepared with the culture medium (Mueller Hinton Agar) and left to gel for 24 hours before inoculating Enterococcus Faecalis ATCC 29212 using the swab technique. Then, 4 cavities of 5 mm in diameter were made, where the epoxy resin-based endodontic sealer (Vioseal), calcium hydroxide-based sealer (Sealer 26), negative control (physiological serum), and positive control (2% chlorhexidine gluconate) were placed. The reading of the bacterial inhibition zones was performed using a digital caliper at 2, 24, 48, and 72 hours. Vioseal showed the greatest statistically significant antibacterial capacity when compared to Sealer 26 (p < 0.05). In conclusion, 2% chlorhexidine gluconate demonstrated the highest antibacterial capacity, followed by Vioseal and Sealer, respectively.
