Examinando por Materia "Enterococcus Faecalis"

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Evaluation of the antibacterial capacity of two endodontic cements based on Calcium Hydroxide and Zinc Oxide against Enterococcus Faecalis strains. In vitro study.
(Universidad Privada Norbert Wiener, 2020-11-04) Gonzales Rojas, Heidy Aracely; Ordoñez Lopez, Carmen Jenny
The objective of this study was to determine the antibacterial capacity of two endodontic cements based on calcium hydroxide and zinc oxide against Enterococcus Faecalis strains. In this in vitro, experimental, longitudinal, and prospective research, the sample consisted of 40 specimens divided into two experimental groups and two control groups. The agar diffusion test was used to determine the antibacterial capacity. For this, the Enterococcus Faecalis ATCC 29212 strain was inoculated onto Mueller Hinton agar plates, and four equidistant perforations were made where the materials were placed. The antibacterial capacity was evaluated by measuring the inhibition halos at 2, 24, and 48 hours. It was found that chlorhexidine gluconate produced the largest bacterial growth inhibition halos, which were statistically significant (p<0.05). When comparing the endodontic cements, it was found that the calcium hydroxide-based endodontic cement produced the largest bacterial growth inhibition halos, which were statistically significant compared to the zinc oxide-based endodontic cement (p<0.05). Conclusion: The calcium hydroxide-based endodontic cement demonstrated better antibacterial capacity against Enterococcus Faecalis.
Acceso abierto
In vitro evaluation of the antibacterial capacity of sealants endodontics based on epoxy resin and calcium hydroxide versus Enterococcus faecalis
(Universidad Privada Norbert Wiener, 2023-09-07) Alvarez Vilca, Roel; Jaime Okumura, Roberto
The objective of this research was to determine, through an in vitro study, the antibacterial capacity of an epoxy resin and calcium hydroxide-based endodontic sealer against Enterococcus Faecalis. Materials and Methods: The antibacterial capacity was evaluated using the disc diffusion test. For this, 14 Petri dishes were prepared with the culture medium (Mueller Hinton Agar) and left to gel for 24 hours before inoculating Enterococcus Faecalis ATCC 29212 using the swab technique. Then, 4 cavities of 5 mm in diameter were made, where the epoxy resin-based endodontic sealer (Vioseal), calcium hydroxide-based sealer (Sealer 26), negative control (physiological serum), and positive control (2% chlorhexidine gluconate) were placed. The reading of the bacterial inhibition zones was performed using a digital caliper at 2, 24, 48, and 72 hours. Vioseal showed the greatest statistically significant antibacterial capacity when compared to Sealer 26 (p < 0.05). In conclusion, 2% chlorhexidine gluconate demonstrated the highest antibacterial capacity, followed by Vioseal and Sealer, respectively.