Examinando por Materia "Fermented Foods"

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    Efecto antagónico in vitro de Lactobacillus spp. aislados de "tocosh" – Solanum sp., proveniente de Huánuco, frente a bacterias enteropatógenas
    (Universidad Privada Norbert Wiener, 2019-11-29) Gutiérrez Rodríguez, Ivette Evelyn; Gavilán Chávez, Ronnie Gustavo
    According to the WHO, diarrheal diseases (DD) are the second leading cause of death in children under five worldwide. Since the Inca era, ‘tocosh’ has been characterized by its nutraceutical properties. The objective of this research was to evaluate the antagonistic effect of Lactobacillus spp. isolated from ‘tocosh’ - Solanum sp. from Huánuco, against enteropathogenic bacteria: Escherichia coli enteropathogenic (EPEC), Salmonella typhi, Shigella flexneri, and Vibrio cholerae Inaba. Methods: The population was extracted from tocosh produced in fermentation pits located in the province of Yarowilca, Obas district. The bacteria were isolated in Man Rogosa Sharpe broth, under anaerobic conditions, and incubated for 48 hours. To purify the Lactobacillus spp. strains, tests were conducted according to Bergey's Manual. In the evaluation of the antagonistic effect, the Burkholder method and the cell-free supernatant (CFS) method were used. The assay was performed in triplicate at a concentration of 2 on the MacFarland scale of lactic acid bacteria, incubated in aerobiosis at 37 °C for 18 hours. Results: Of a total of 223 samples, 44 strains showed microscopic and biochemical characteristics compatible with Lactobacillus spp. The mean inhibition halos formed by the Burkholder method were 11.36 mm against Vibrio cholerae Inaba 1.457.10, 9.86 mm against Salmonella typhi 2.240-16, 6 mm against Shigella flexneri WHO SH-15.3, and 6 mm against Escherichia coli enteropathogenic (EPEC) 2.734-18. The cell-free supernatant method obtained means of 13.62 mm against Vibrio cholerae Inaba 1.457.10, 10.52 mm against Salmonella typhi 2.240-16, 6.33 mm against Shigella flexneri WHO SH-15.3, and 6.19 mm against Escherichia coli enteropathogenic (EPEC) 2.734-18. A comparison of groups determined that the greatest effect was obtained against Vibrio cholerae Inaba by both methods. Conclusions: It was determined that Lactobacillus spp. isolated from ‘tocosh’ presented an antagonistic effect against the reference enteropathogenic bacteria that cause diarrheal diseases.