Examinando por Materia "Genes"

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Acceso abierto
Análisis de perfiles genéticos mezcla y su aplicación en evidencias forenses de violencia sexual de mujeres peruanas en UNBIMOG 2019-2020
(Universidad Privada Norbert Wiener, 2022-10-22) Bermejo Valladolid, Maria Estilita; Montellanos Cabrera, Henry Sam
For the analysis of genetic mixture profiles, the objective of this study was to apply interpretation criteria of the analysis of genetic mixture profiles, in forensic evidence of sexual violence of Peruvian women, in the Molecular and Genetic Biology Unit; 2019-2020. The research design is non-experimental, applied, cross-sectional and descriptive. 141 forensic evidence from 36 criminalistics cases were analyzed where it was possible to determine that the genetic profiles met the criteria for general interpretation of mixed profiles, for which 98% exceeded the analytical threshold (≥ 50 RFU), 82% exceeded the threshold stochastic (≥ 150-200 RFU), 60% exceeded the stutter threshold (≥ 10-15% RFU), 92% presented heterozygote imbalance (< 0.6), the proportion of contributors 0.1-1 was distinguishable in 62 %, the extrabands recognizable in 60% were the stutters, the presence of genetic artifacts was in 44% and the stochastic, degraded and/or inhibited profiles are related to the types of mixture profiles. In the criminal cases of a single aggressor, they met the general thresholds of scientific recommendation, likewise the mixed profiles were interpretable, the conclusive cases were in 78 pieces of evidence and the inconclusive cases were in 13 pieces of evidence. In the criminal cases of multiple aggressors, the mixed profiles met the general thresholds of scientific recommendation, likewise these profiles were interpretable, the conclusive cases were 41 pieces of evidence and the inconclusive cases were 9 pieces of evidence.
Acceso abierto
Perfil genético en prendas procesadas con pruebas de identificación y diagnóstico hematológico en la Unidad Médico Legal II–Lima Norte, 2023
(Universidad Privada Norbert Wiener, 2023-07-18) Sarmiento Yengle, Valery Roxana; Casana Jara, Kelly Milagritos
The Forensic Biology Laboratory of the Legal Medicine Unit of North Lima has been processing different samples, in order to find evidence that can help in forensic investigations. This has been using hematological identification and diagnostic tests such as the Adler Test, Hexagón Obti and Bluestar to determine the presence of blood, which can be transported for genetic analysis; For this reason, the main objective of this investigation was to determine if the previous use of hematological identification and diagnostic tests affects the subsequent obtaining of the genetic profile in processed garments, using an applied methodology, of a quantitative approach and of a non-experimental design, with a sample of 13 garments analyzed in both forensic laboratories. Once these data were obtained, they were analyzed using the IBM SPSS STATICS Software, version 20. Likewise, for the hypothesis test, Fisher's exact test was used, derived from a Pearson Chi-square analysis; having as results the amplification of 100% of the samples analyzed with the Adler Test and 88.9% of the samples analyzed with Bluestar, however, it was not possible to obtain results from the Hexagón Obti since the samples worked with this reagent were not analyzed. in the laboratory of Molecular Biology and Genetics. Coming to the conclusion that the previous use of hematological identification and diagnostic tests does not affect the subsequent obtaining of the genetic profile in processed garments, because the genetic profile of 92.3% of all the samples analyzed was obtained.