Examinando por Materia "Germinal Center"

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    Egg Albumin Germ Tube Test Performance in Identifying Candida Albicans, 2020
    (Universidad Privada Norbert Wiener, 2020-03-11) Arenas Pillco, Liliana Veronica; Rosas Paytan, Ruth Victoria; Rojas León, Roberto Eugenio
    Candida albicans identification is performed with the germ tube test using human serum, which, despite being accessible, is not biosecure. Various researchers have conducted this test using egg albumin with satisfactory results but did not measure its performance. The aim of this study was to evaluate the performance of the germ tube test with egg albumin in identifying Candida albicans. This was a quantitative, observational, analytical, cross-sectional study conducted at the Norbert Wiener University Laboratory, following INS protocols. The germ tube test was performed using egg albumin from Gallus gallus domesticus, employing 118 Candida albicans strains and 12 non-Candida albicans strains. Sensitivity, specificity, false positives, false negatives, and efficiency were: 95.8%, 100%, 0%, 4.2%, and 96.2%, respectively. The Kappa index was 0.81, indicating high agreement with the reference method. Following SEIMC criteria, it was concluded that the test using egg albumin is acceptable for identifying Candida albicans and is an economical, accessible, and, above all, safe medium for laboratory personnel.