Examinando por Materia "Hemolysis"

Mostrando 1 - 3 de 3

Acceso abierto
Evaluation of human blood agar culture medium with washed red blood cells for the development of Streptococcus agalacti strains.
(Universidad Privada Norbert Wiener, 2020-10-01) Cano Corales, Katia Alejandra; Ugarte Córdova, Ana Margarita; Borja Velezmoro, Gustavo Adolfo
The study evaluated the performance of an alternative culture medium, human blood agar with 5% washed red blood cells, for the development of Streptococcus agalactiae strains compared to 5% sheep blood agar. The sample consisted of 36 properly identified Streptococcus agalactiae strains. Results obtained from the ASHGL 5% medium showed 100% sensitivity and specificity after 24 hours of incubation. Positive and negative predictive values also reached 100%. The morphology of the colonies and the presence of beta-hemolysis on both sheep blood agar and ASHGL 5% agar were evident after 24 hours of incubation. Conclusion: The human blood agar with 5% washed red blood cells (ASHGL) is as effective as 5% sheep blood agar.
Acceso abierto
Interference due to hemolysis in the measurement of biochemical constituents in two types of analyzers. Dry Chemistry vs. Liquid Chemistry – 2023
(Universidad Privada Norbert Wiener, 2023-11-19) Oncebay Segura, Jackeline Zully; Saldaña Orejón, Italo Moisés
Introduction: Hemolysis in diagnostic samples is one of the leading causes of clinically significant errors in the measurement of biochemical parameters. Objective: To determine the effect of hemolysis interference in the measurement of biochemical constituents in dry chemistry analyzers (Vitros 7600®) and liquid chemistry analyzers (Atellica® Solution). Methods: Pre-experimental study with pre- and post-test. Increasing amounts of hemoglobin were added to seven aliquots of a serum mixture, and the interference on 22 constituents was determined in triplicate. The relative deviation percentage of the constituent's concentration due to hemolysis was calculated compared to a sample without interference. Tolerance limits for interference were established using the desirable systematic error criterion. Results: Significant interference was detected in 16 of 22 constituents for the liquid chemistry analyzer, while 17 constituents were affected by hemolysis on the dry chemistry analyzer according to the tolerable limit criterion used. The constituents with the highest biases for the liquid chemistry analyzer were LDH (+302.60%), AST (+102%), potassium (+44.82%), and CK (+27.66%), while for the dry chemistry platform, the affected constituents were LDH (+223.28%), AST (+109.43%), total bilirubin (+61.02%), and potassium (+44.44%). Conclusions: The discordant results based on the methodology and analyzer used highlight the need to harmonize processes and establish identical interference limits across laboratories and suppliers.
Acceso abierto
Percentage of hemolysis in red blood cell concentrates during their storage in the blood bank of the Delgado Clinic, period August - September 2019
(Universidad Privada Norbert Wiener, 2021-08-12) Yataco Poma, Jessica Jane; Arocutipa Ccopa, María Elena; Palacios Butrón, Fernando Sarco
The blood storage system for red blood cell concentrates in a blood bank aims to counteract the damage caused by the metabolic machinery within the hematocyte membrane to improve its post-transfusion viability.