Examinando por Materia "Hepacivirus"

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Diagnostic usefulness of the electrochemiluminescence method versus real-time PCR for hepatitis C diagnosis in a Lima dialysis center, 2018
(Universidad Privada Norbert Wiener, 2019-12-06) Herrera Crispín, Diana Irma; Peña Santa Cruz, Elma; Navarrete Mejía, Pedro Javier
This study aimed to identify the diagnostic utility of the ECLIA assay method compared to the PCR-RT method for diagnosing Hepatitis C at a dialysis center in Lima in 2018. The study was observational, cross-sectional, and retrospective, analyzing results from dialyzed patients at a renal health center in Lima. The study included results from immunoserological tests using the ECLIA method and PCR-RT for patients with multiple etiologies, excluding samples with questionable or indeterminate immunoserological results, neonatal sera, and patients under 18 years old. The first detection method for HCV was ECLIA technology, and PCR-RT was used as the gold standard. Sensitivity and specificity, PPV, and NPV were calculated to determine the validity and diagnostic security of the ECLIA method compared to PCR-RT.Of 123 patients with results from both tests between January and December 2018, 45% were reactive by ECLIA (55/123) and 17% were detectable by PCR-RT (21/123). Sensitivity was 90% (19 reactive by ECLIA out of 21 detectable by PCR-RT), specificity was 65% (66 non-reactive by ECLIA out of 102 non-detectable by PCR-RT), PPV was 93%, and NPV was 57%. The Cohen Kappa index was 0.34, with a concordance level of 69.1% between both tests, excluding random coincidences. The study concluded that the ECLIA method is highly sensitive and moderately specific for HCV in dialyzed patients, with a high degree of diagnostic security for confirming both sick and healthy patients. The degree of concordance in the diagnosis between ECLIA and the gold standard was moderate.