Examinando por Materia "Klebsiella pneumoniae"

Mostrando 1 - 2 de 2

Acceso abierto
Efficacy of high-level disinfection in safe disinfection of endoscopes
(Universidad Privada Norbert Wiener, 1905-07-09) Rubio Ampuero, Rosa Elena; Gómez Gonzales, Walter Edgar
Objective: Determine the effectiveness of High Level Disinfection (HLD) in the safe disinfection of endoscopes. Methodology: Systematic review whose population is made up of 06 published and indexed scientific articles, data collection was carried out through Lilacs, Scielo, Cochrane Plus, Medline, the analysis was carried out through a summary table, evaluating the quality of evidence and strength of recommendation in a critical and intensive manner for each article according to the established technical criteria, guaranteeing compliance with ethical principles. Results: At the national level, the Herediana Medical Journal concludes that the bacterial load before the cleaning and disinfection process of 50 endoscopes was positive in 88% and after the process positive in 26%. At the international level, the Chilean Journal of Infectology refers to infections exogenous pneumonias such as post-bronchoscopy pneumonia, which reach incidence rates, according to the authors, between 0.6% and 6.9% of the procedures.(6).The Spanish Society of Gastroenterology refers in 02 studies that DAN is not sufficient, both for endoscopes and accessories. The predominant bacteria in the positive cultures were Pseudomona Aeruginosa and Klebsiella Pneumoniae. Conclusion: The systematic review shows that DAN is not sufficient for the safe disinfection of endoscopes in sterilization centers.
Acceso abierto
Phenotypic and molecular characterization of strains producing extended-spectrum beta-lactamases isolated from pediatric patients, 2018
(Universidad Privada Norbert Wiener, 2019-07-30) Ancajima Ballena, Maria Elena; Sotelo Alejos, Rocío Maribel; Benites Azabache, Juan Carlos
The resistance to beta-lactams in the pediatric population has not been previously evaluated in our community. This research aimed to characterize phenotypically and molecularly the extended-spectrum beta-lactamase (ESBL)-producing strains isolated from pediatric patients during the second semester of 2018. The study was prospective, cross-sectional, and descriptive, involving 30 pediatric patient samples. Data analysis was performed using the SPSS v21.0 statistical package, and tables and graphs were created in MS-Excel 2010. ESBL-producing strains were identified using antimicrobial susceptibility methods and CLSI breakpoint criteria, and the genes blaTEM, blaSHV, and blaCTX were characterized using conventional PCR. Most of the samples analyzed were urine (90%), with E. coli (76%) and K. pneumoniae (10%) being the most frequent bacterial agents. E. coli strains presented the blaCTX gene (87%), the blaTEM gene (83%), and the blaSHV gene (74%). Two strains (66.7%) of K. pneumoniae presented the blaCTX and blaTEM genes simultaneously, and two strains (66.7%) of P. mirabilis presented the blaCTX gene. The phenotypic and molecular characteristics of ESBL-producing strains isolated from pediatric patients indicated bacterial resistance, with the blaCTX gene being the most frequently found.