Examinando por Materia "Leukocytes, Mononuclear"

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    Evaluation of the performance of lysants for the study of immunophenotype by full spectrum flow cytometry in peripheral blood in a private laboratory, Lima -2023
    (Universidad Privada Norbert Wiener, 2023-11-26) Huamanyalli Villar, Elizabeth; Champa Guevara, Cesar Alfonso
    The study of immunophenotyping by Flow Cytometry provides relevant information for diagnosing and monitoring hematological neoplasms. Therefore, protocols for immunophenotyping are based on the use of lysing solutions to eliminate red blood cells, reducing interference effects in data interpretation during subsequent analysis. Selecting an appropriate lysing solution based on EuroflowTM standardization criteria is crucial. The incorporation of new technologies into the laboratory, such as full-spectrum cytometry, involves evaluating conditions to verify the working platform (including the method, protocol, and reagents). Proper use of the lysing agent reduces variability in event dispersion. Furthermore, developing and implementing standardized protocols that allow laboratories to provide reproducible and reliable results is considered good practice.