Examinando por Materia "Propolis"
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Publicación Acceso abierto Actividad antibacteriana del propóleo y su combinación con el hidróxido de calcio sobre el Enterococcus faecalis. Estudio in vitro 2021.(Universidad Privada Norbert Wiener, 2022-06-10) Perez Llantoy, Linda Katherine; Vilchez Bellido, DinaEl propósito de este estudio fue determinar la actividad antibacteriana del extracto de propóleo al 30 % y su combinación con el hidróxido de calcio sobre cepas de Enterococcus Faecalis ATCC 19433. La muestra estuvo conformada por cultivos de Enterococcus Faecalis ATCC 19433, 4 grupos de 25 cultivos, tres de pastas experimentales (Hidróxido de calcio “PrevestDenPro” 70%, Extracto de Propóleo 30% e Hidróxido de calcio 70% más propóleo 30%), y un control negativo (agua destilada), midiéndose los halos de inhibición a las 24 y 48 horas. Como resultado se encontró que hubo actividad antibacteriana en las tres pastas experimentales (halos de inhibición > 0): hidróxido de calcio 70% (M: 7,164 y M: 7,664), hidróxido de calcio 70% más propóleo 30% (M: 7,804 y M: 8,184) y en el extracto de propóleo 30% (M: 12,848 y M: 13,428), a las 24 y 48 horas, respectivamente. Se encontró diferencias significativas entre los halos de inhibición a las 24 y 48 horas, para cada sustancia (p valor < 0.05); al compararlas en pares, hubo diferencias significativas entre el extracto de propóleo y el hidróxido de calcio (sig: 0.00), y el hidróxido de calcio más extracto de propóleo y el extracto de propóleo (sig: 0.00), sin diferencia significativa entre el hidróxido de calcio y el hidróxido de calcio más extracto de propóleo (sig: 0.080 y 0.484, a las 24 y 48 horas). En conclusión, existe efectividad antibacteriana del extracto de propóleo 30 % y de su combinación con hidróxido de calcio sobre cepas de Enterococcus Faecalis ATCC 19433, a las 24 y 48 horas, siendo el extracto de propóleo 30%, quien mostró mayor actividad antibacteriana.Publicación Acceso abierto Actividad antifúngica del extracto de propóleo frente a Candida albicans de pacientes con candidiasis vulvovaginal en el año 2021(Universidad Privada Norbert Wiener, 2022-06-15) Rodríguez Loyola, Gina Andrea; Collanque Pinto, Jesús DanielThe general objective of this research was to evaluate the antifungal activity of propolis extract against Candida albicans from patients with vulvovaginal candidiasis in 2021. The research used a quantitative approach with an experimental design. The population consisted of C. albicans strains isolated from patients with vulvovaginal candidiasis admitted to the Gynecology Service of a Private Clinic in Lima. A total of 34 strains were chosen (17 C. albicans strains from acute cases and 8 from recurrent cases) and were treated with propolis extract and fluconazole, with three repetitions, resulting in 102 experimental units. The antifungal susceptibility test was performed using the disk diffusion method with a single concentration of propolis extract and fluconazole as the positive control. Results: The propolis extract, like fluconazole, inhibited all C. albicans strains. However, the effect of fluconazole was significantly greater than that of the research product. Conclusion: The propolis extract does show antifungal activity against C. albicans.Publicación Acceso abierto Caracterización fisicoquímica de la miel y propóleo de la abeja nativa sin aguijón (trigona angustula) y elaboración de granola, caramelo y marshmallow. Lima. 2022(Universidad Privada Norbert Wiener, 2023-04-14) Sandoval Guerrero, Anthony; Guadalupe Sifuentes de Posadas, Luz FabiolaIt is well known that honey and propolis from various bees are products used as alternative and complementary medicine to treat various health conditions. The objective of the study on honey and propolis from the native stingless bee (Trigona angustula) is to analyze their physicochemical characteristics and to produce candies based on the products provided by this insect, such as granola, caramel, and marshmallow made from honey and propolis. Additionally, the study aims to highlight the importance of native stingless bees in pollination, not only for agricultural crops but also for their crucial role in ecosystems, as they are the primary pollinators of tropical and subtropical forests in the region. The purpose of this study is also to raise awareness about protecting stingless bees, as excessive use of pesticides, insecticides, and chemical products in agriculture, combined with deforestation, is leading to their extinction. Samples of honey and propolis from native stingless bees will be collected in the northeastern region of Peru, specifically in the Cajamarca region, Jaén province, Las Pirias district, located at 1625 meters above sea level. For honey, analyses will be conducted on individual and total sugars using a 600g sample and the Separation of Sugars in Honey method; reducing sugars using a 100g sample and the Determination of Reducing Sugar Content method; ash content with a 100g sample and the Determination of Mineral Substances Content method; hydroxymethylfurfural (HMF) content using milligrams of honey and the Spectrophotometric Determination of Hydroxymethylfurfural method; moisture content with a 100g sample and the Determination of Moisture Content method; protein content with a 100g sample using the Crude Protein-Combustion Method; and pH (at 25°C) using the pH of Acidified Foods method. For propolis, analyses will include metals using ICP and atomic absorption by the Inductively Coupled Plasma Atomic Emission Spectrometric Method for Trace Element Analysis of Water and Wastes; mercury and arsenic using methods for determining cadmium, arsenic, lead, tin, copper, iron, zinc, and mercury in foods through atomic absorption spectrophotometry (Hg, As); ash content with a 100g sample using the Determination of Mineral Substances Content method; moisture content with a 100g sample using the Solid in Water method; and pH (at 25°C) using the pH Acidified Foods method
