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Interference due to hemolysis in the measurement of biochemical constituents in two types of analyzers. Dry Chemistry vs. Liquid Chemistry – 2023
(Universidad Privada Norbert Wiener, 2023-11-19) Oncebay Segura, Jackeline Zully; Saldaña Orejón, Italo Moisés
Introduction: Hemolysis in diagnostic samples is one of the leading causes of clinically significant errors in the measurement of biochemical parameters. Objective: To determine the effect of hemolysis interference in the measurement of biochemical constituents in dry chemistry analyzers (Vitros 7600®) and liquid chemistry analyzers (Atellica® Solution). Methods: Pre-experimental study with pre- and post-test. Increasing amounts of hemoglobin were added to seven aliquots of a serum mixture, and the interference on 22 constituents was determined in triplicate. The relative deviation percentage of the constituent's concentration due to hemolysis was calculated compared to a sample without interference. Tolerance limits for interference were established using the desirable systematic error criterion. Results: Significant interference was detected in 16 of 22 constituents for the liquid chemistry analyzer, while 17 constituents were affected by hemolysis on the dry chemistry analyzer according to the tolerable limit criterion used. The constituents with the highest biases for the liquid chemistry analyzer were LDH (+302.60%), AST (+102%), potassium (+44.82%), and CK (+27.66%), while for the dry chemistry platform, the affected constituents were LDH (+223.28%), AST (+109.43%), total bilirubin (+61.02%), and potassium (+44.44%). Conclusions: The discordant results based on the methodology and analyzer used highlight the need to harmonize processes and establish identical interference limits across laboratories and suppliers.