Examinando por Materia "Pruebas de Sensibilidad Microbiana"

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Acceso abierto
Actividad antimicrobiana del aceite esencial de Origanum vulgare L. “orégano peruano” frente a Staphylococcus aureus
(Universidad Privada Norbert Wiener, 2018-05-31) Salinas Segura, Roger; Carhuapoma Yance, Mario
The objective of this research was to confirm the antimicrobial activity of the essential oil of Origanum vulgare L., "Peruvian Oregano," against Staphylococcus aureus. The essential oil was obtained by steam distillation and purified with anhydrous sodium sulfate. The analysis of the main phytochemical components of the oil was performed using organoleptic and instrumental methods. The antimicrobial activity was assayed against Staphylococcus aureus ATCC 6538 using the disk diffusion method (Kirby-Bauer), with Gentamicin 10 µg (OXOID brand) as the positive control and dimethyl sulfoxide as the negative control. Results: The essential oil had a good yield of 2.02%, distilled in 60 minutes, with a density of 0.9132 g/mL and a refractive index of 1.475. The main components were carvacrol and thymol. The essential oil of Origanum vulgare L. showed antimicrobial activity against Staphylococcus aureus ATCC 6538, with inhibition halos of 11.47,13.40,16.60, and 19.65 mm at concentrations of 25,50,75, and 100 µg/mL, respectively. The gentamicin control resulted in an inhibition halo of 30.14 mm. Conclusion: The highest antimicrobial activity of the essential oil of Origanum vulgare L. "Peruvian Oregano" against Staphylococcus aureus was confirmed at 100 µg/mL.
Acceso abierto
Actividad antimicrobiana del extracto etanólico de las hojas de Schinus molle L. "molle"
(Universidad Privada Norbert Wiener, 2017) Clemente Sotteccani, Claudia Elizabeth; Paucar Lopez, Rosmery; Chávez Flores, Juana Elvira
Peru is one of the countries with the greatest biodiversity in the world, and many of its plant species can be sustainably utilized by industry. Schinus molle L. ("Molle"), a native tree with medicinal applications whose ethanolic extract exhibits antibacterial properties, is a clear example. The objective of this research was to confirm the antimicrobial activity of the ethanolic extract from the leaves of Schinus molle L. To this end, the ethanolic extract was obtained at concentrations of 500 and 1000 mg/mL, with 0.12% chlorhexidine gluconate as the positive control and distilled water as the negative control. The study of antimicrobial activity was conducted using the disk diffusion method with surface plating technique. The experimental solutions were incubated under anaerobic conditions for 48 and 72 hours at 37∘ C, after which the diameters of the inhibition halos were read with a vernier caliper. A qualitative analysis determined the presence of metabolites: flavonoids, alkaloids, carbohydrates, steroids and/or triterpenes, reducing sugars, and phenolic compounds. Results: The ethanolic extract at concentrations of 500 and 1000 mg/mL demonstrated antimicrobial activity against Streptococcus mutans ATCC 25175. From the comparison performed, chlorhexidine gluconate produced greater inhibition. Conclusion: The ethanolic extract of Schinus molle L. 'Molle' presents antimicrobial activity against Streptococcus mutans ATCC 25175, and the chlorhexidine gluconate is qualitatively similar to the ethanolic extract of Schinus molle L. 'Molle'.
Acceso abierto
Determinación fitoquímica y actividad antimicrobiana del aceite esencial de los órganos aéreos de Lepechinia meyenii (Walp.) Epling "pacha salvia"
(Universidad Privada Norbert Wiener, 2018-03-09) Quispe Silva, Mirela Magaly; Tenorio Roca, Eduardo; Carhuapoma Yance, Mario
The plant species Lepechinia meyenii (Walp.) Epling (commonly known as salvia, pacha salvia, salvia de puna, kotapuriña) is a native plant that grows in the southern Andean regions of Peru (Ayacucho, Huancavelica, Cuzco, and Puno). The main objective of the present study was to conduct a phytochemical evaluation and determine the antimicrobial activity of the essential oil obtained from the aerial parts of Lepechinia meyenii (Walp.) Epling “pacha salvia.” In the methodology, a gas chromatograph coupled with a mass spectrometer (GC-MS) was employed to determine the phytochemical composition, and the agar diffusion method (Kirby-Bauer) was used to evaluate antimicrobial activity. The results showed the presence of 31 components, with the major compounds being α-pinene (21.37%), eucalyptol (1,8-cineole = 18.80%), and D-limonene (15.43%). The essential oil demonstrated antimicrobial activity against Staphylococcus aureus ATCC 6538 when compared with gentamicin 10 µg. It is concluded that the essential oil from the aerial parts of Lepechinia meyenii (Walp.) Epling “pacha salvia” contains active components with antimicrobial activity.
Acceso abierto
Evaluación de la efectividad antimicrobiana de un colutorio a base de los aceites esenciales de Eucalyptus globulus Labill (eucalipto) y Minthostachys sp. (muña), frente a Klebsiella pneumoniae ATCC 10031, Staphylococcus aureus ATCC 29737 y Candida albicans ATCC 10231
(Universidad Privada Norbert Wiener, 2017-12-06) Aylas Canicela, Roosevelt Edhair; Nué Martinez, Jorge Jonathan
The present research work entitled “Evaluation of the antimicrobial effectiveness of a mouthwash based on the essential oils of Eucalyptus globulus Labill (Eucalyptus) and Minthostachys sp. (Muña), against Klebsiella pneumoniae ATCC 10031, Staphylococcus aureus ATCC 29737 and Candida albicans ATCC 10231”, had as its main objective to evaluate the antimicrobial effectiveness of the formulated mouthwash. The study was quasi-experimental, prospective, and cross-sectional. A registration form was used for the data obtained. The method employed for the evaluation of the antibacterial effectiveness was the Modified Microbial Challenge test. The results were: for Klebsiella pneumoniae, at a significance level of 0.05 and a p-value = 0.4412, the treatment with the formulated mouthwash has the same effect as the commercial mouthwash; for Staphylococcus aureus, at a significance level of 0.05 and a p-value = 0.6039, the treatment with the formulated mouthwash is not more effective than the commercial mouthwash, both present the same effectiveness; for Candida albicans, at a significance level of 0.05 and a p-value = 0.0000325, it is accepted that the treatment with the formulated mouthwash shows greater effectiveness than the commercial mouthwash.
Acceso abierto
Validación del método microbiológico de cilindro-placa para la cuantificación de gentamicina sulfato en Dermolab NF crema de Laboratorios Vitapharma S.A.C., Lima 2018
(Universidad Privada Norbert Wiener, 2019-06-11) Huerto Huapaya, Carmen Aracelli; Vignolo Asencio, Luis Ernesto; Guevara Vicaña, Rita Aurora
The present study was conducted with the objective of standardizing a microbiological cylinder-plate method for the quantification of gentamicin and subsequently validating it, that is, to demonstrate through documented evidence in the Quality Control Laboratory of Vitapharma S.A.C. that the product Dermolab NF cream meets the required validation criteria. The validation process was described and developed using the agar diffusion method, with Staphylococcus epidermidis ATCC 12228 as the test microorganism. The analytical parameters evaluated included specificity (selectivity), linearity, range, accuracy, and precision. Statistical evaluation of the experimental results, based on the established acceptance criteria, demonstrated that the analytical method is specific, linear (r² = 0.98 for the standard and r² = 0.97 for the sample, with a minimum specification of 0.95), precise (CV = 2.03% and 1.73%, with a specification of <5%; tolerance = 19.15% and 16.28%, corresponding to repeatability and intermediate precision, respectively, with a specification of <30%), and accurate (bias = 1.06%, with a specification of <3%; G_exp = 0.511 < G_tab = 0.871; T_exp = 0.765 < T_tab = 2.306) within the studied concentration range. Therefore, based on the studies performed, it is established that the analytical performance characteristics meet the requirements for the proposed analytical application, providing reliable results for verifying the quality specifications of the product.