Examinando por Materia "Ratas"

Mostrando 1 - 4 de 4

Acceso abierto
Actividad antiulcerosa del extracto etanólico de las hojas de Ficus carica L. "Higo" en ratas
(Universidad Privada Norbert Wiener, 2018-09-17) Abarca Vega, Erika Victoria; Chávez Flores, Juana Elvira
Objective: To determine the antiulcer activity of the ethanolic extract of Ficus carica L. (“Fig”) leaves in rats. An ethanolic maceration of the plant leaves was carried out, followed by solubility testing, preliminary phytochemical analysis, and evaluation of antiulcer activity using the modified Lee (1971) technique at doses of 300, 600, and 800 mg/kg. A total of 60 Holtzman rats were randomly distributed into six groups, induced with gastric ulcer using naproxen 200 mg/kg, with ranitidine 30 mg/kg as the reference drug. The extract was shown to be soluble in methanol, ethanol, and distilled water. The preliminary phytochemical screening revealed the presence of flavonoids, alkaloids, phenolic compounds, and free amino groups. Regarding the antiulcer activity, the most effective treatment was the Ficus carica L. leaf extract at 800 mg/kg, achieving 83.01% inhibition of gastric ulceration, compared with ranitidine, which achieved 73.58% inhibition. These results were obtained using the Marhuenda scale. It was determined that the ethanolic extract of Ficus carica L. leaves at a dose of 800 mg/kg presents antiulcer activity in rats.
Acceso abierto
Efecto cicatrizante del gel formulado a partir del extracto hidroalcohólico de Ligaria cuneifolia R&P., "suelda con suelda" en ratas
(Universidad Privada Norbert Wiener, 2021-12-07) Munares Cusi, Milton Ever; Cayao Regalado, Maribel Soledad; Alvarado Chávez, Britt
In Peru, medicinal plants are of great importance for research into new formulations due to being a natural alternative. Objective: To confirm the wound-healing effect of the gel formulated from the hydroalcoholic extract of Ligaria cuneifolia R&P., 'suelda con suelda,' in rats. Methodology: The plant species was collected in the San Pedro community, Puywas district, Huancayo department. A hydroalcoholic extraction of the Ligaria cuneifolia R&P leaves was performed. A solubility test and phytochemical screening were conducted. The wound-healing activity was executed using three gel concentrations (0.5%,5%, and 10%) prepared from the hydroalcoholic extract. This was compared with standard groups based on Contractubex® Gel (onion plant cells) and a base gel control group, culminating in the anatomopathological technique (histological sections of the healing skin tissue). Results: The existence of secondary metabolites such as flavonoids, tannins, and phenolic compounds was evidenced. Regarding the wound-healing effect, the gel based on the 10% hydroalcoholic extract of Ligaria cuneifolia R&P presented the highest healing efficacy (histologically, the presence of reparative changes, marked hyperplasia of basal cells, and superficial and deep dermis with moderate fibrosis and an increase in fibroblasts and cicatricial epithelium was observed). The histological findings showed scar reparative changes in the epidermis, with mild fibrosis in the dermis, indicating a good healing response. The healing degree was rated as Good, validating 80.6% efficacy compared to Contractubex®, which reached 70.6% efficacy. Conclusion: It was confirmed that the hydroalcoholic extract of Ligaria cuneifolia R&P 'suelda con suelda' presents wound-healing activity in rats.
Acceso abierto
Efecto del extracto hidroalcohólico de hojas frescas de Mutisia acuminata R. & P. “chinchilcuma” en la hiperplasia benigna prostática inducida por el alcohol etílico en ratas
(Universidad Privada Norbert Wiener, 2013) Abarca Caqui, Aida Verónica; Chávez Flores, Juana Elvira
In the present research work, the effect of the hydroalcoholic extract of fresh leaves of Mutisia acuminata R. & P. (“Chinchilcuma”) was evaluated in benign prostatic hyperplasia (BPH) induced by ethyl alcohol in Holtzmann strain rats. To determine the secondary metabolites of this plant species, the hydroalcoholic extract of fresh leaves of Mutisia acuminata R. & P. was employed. The species was collected in January 2011 in the community of Huaruccña, located at 3200 meters above sea level, in the district of Coracora, province of Parinacochas, department of Ayacucho, Peru. A phytochemical study was conducted, including solubility tests, phytochemical analysis, and structural elucidation using spectroscopic methods (UV/Vis and IR), which revealed the presence of a secondary metabolite: a flavonoid of the flavonol type. To verify the effect of the hydroalcoholic extract, benign prostatic hyperplasia was induced in the experimental animals through ethyl alcohol administration for 65 days. Morphological parameters of the prostate gland showed that treatment with 150 mg/kg of the extract achieved 38.5% efficacy in reducing prostate weight, with results comparable to those obtained with finasteride at 5 mg/kg (36.5%) and Cefasabal® at 25 mg/kg (32.7%). Biochemical analysis of acid phosphatase indicated a reduction in the group treated with 150 mg/kg of extract (0.014 ng/dL) compared with the BPH group (0.017 ng/dL). Immunological analyses showed that the group treated with 150 mg/kg of extract had free testosterone levels of 0.9 pg/dL compared to 1.0 ng/dL in the BPH group, while prostate-specific antigen free (PSAL) levels were 0.005 ng/dL in the treated group versus 0.008 ng/dL in the BPH group. Histopathological sections of the group treated with 150 mg/kg of extract revealed fewer features of BPH, yielding results similar to those observed with the synthetic drugs Cefasabal® at 25 mg/kg and finasteride at 5 mg/kg.
Acceso abierto
Evaluación de toxicidad aguda del extracto etanólico del tubérculo Euphorbia huanchahana Klotzch & Garcke Boissier “huachangana” en ratas Holtzman
(Universidad Privada Norbert Wiener, 2020-01-22) Quispe Llactahuamán, Pilar Alicia; Villafuerte Pedraza, Graciela; Ñañez del Pino, Daniel
Poisoning from medicinal plants can occur due to the improper use of an apparently harmless natural substance, or as a result of absorbing an excessive amount of a phytosanitary product in a short period. The objective was to determine the acute hepatic and renal toxicity of the ethanolic extract of Euphorbia huanchahana Klotzch & Garcke Boissier, 'Huachangana,' in Holtzman rats. Methodology: An experimental study was designed, and a qualitative phytochemical profile analysis was conducted to determine the presence of primary and secondary metabolites. For the acute toxicity test, a dose of 2000 mg/kg (LD50) was administered orally in a single dose to 32 rats. Four rats were administered only distilled water. The animals remained under observation for 14 days, during which no signs of toxicity, alteration of the growth curve, or death were detected. On day 15, euthanasia was performed, and organs were removed for histopathological analysis of the liver and kidney tissues. A liver profile analysis was also conducted. Conclusion: The ethanolic extract of the Euphorbia huanchahana tuber at a dose of 2000 mg/kg showed no evidence of acute hepatic or renal toxicity in Holtzman rats.