Examinando por Materia "Spectrum Analysis"

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    Development and validation of a test for the measurement of methhemoglobin through the analysis of images with a smartphone - 2023
    (Universidad Privada Norbert Wiener, 2024-07-20) Leon Trujillo, Bryan Estiff; Saldaña Orejón, Italo Moisés
    Objective: To develop and validate a test for measuring methemoglobin using image analysis with a smartphone. Materials and Methods: The design of this research is descriptive cross-sectional, correlational, quantitative, and prospective. It will consist of a set of arterial blood samples collected in lithium heparin tubes at the Carlos Lanfranco La Hoz Hospital in the biochemistry area from September 2023 to January 2024. Results: A linear relationship was found between sodium nitrite concentration and methemoglobin concentration obtained in the ABL-800 Flex gasometer, with an R² value of 0.9971. The values of the red channel showed an inversely proportional relationship with methemoglobin concentration. Image analysis was performed using the regression equation Y = -1.0036x + 132.44. According to the research, methemoglobin determination with a smartphone showed minimal bias compared to hemoglobin levels and methylene blue concentration. Conclusions: A simple and cost-effective method was developed for determining methemoglobinemia through measurement of the red channel values of RGB using a smartphone camera, with minimal interference from hemoglobin levels and methylene blue. This will be very useful in health centers with limited resources or in situations where methemoglobin concentrations cannot be measured rapidly.