Examinando por Materia "Staining and Labeling"

Mostrando 1 - 4 de 4

Acceso abierto
Beta vulgaris as a natural nuclear staining color for the histopathological diagnosis of breast cancer at V&M Clinic Image Medica S.A.C, Lima-Peru, 2023
(Universidad Privada Norbert Wiener, 2023-11-26) Quispe Chancas, Nicol; García Vásquez, Carlos Hugo
Beta Vulgaris is a natural dye with tinting properties useful for staining histological tissues, which is particularly beneficial for histopathological diagnosis in one of the most common conditions among women in our country. The preparation and extraction of the dye are free from toxic substances.
Acceso abierto
Evaluation of a colorimetric method for the detection of residual antimicrobial activity in urine cultures of the National Teaching Hospital Mother Child San Bartolomé, Lima 2023
(Universidad Privada Norbert Wiener, 2023-11-09) Julcarima Briceño, Jhonny Josmell; Astete Medrano, Delia Jessica
Experimental Study: The aim was to determine the antimicrobial activity in urine samples analyzed for uroculture using a colorimetric method. 1,014 urine samples were analyzed to detect residual antimicrobial activity with Bacillus subtilis ATCC 6633 spores as the indicator strain. Results from the colorimetric method showed a frequency of 11.4% in detecting residual antimicrobial activity, with a sensitivity of 97.5%, specificity of 100%, a negative predictive value of 99.6%, and a positive predictive value of 100%. The interpretation time was 66 (6.5%) samples in 2 hours and 50 (4.9%) samples in 4 hours. Conclusion: The colorimetric method showed excellent efficacy based on its sensitivity, specificity, interpretation time, and predictive values, aligning with the disk diffusion method.
Acceso abierto
Performance of the gram and modified Browm - Brenn staining method in the diagnosis of bacterial endocarditis Incor, Lima, 2015-2020.
(Universidad Privada Norbert Wiener, 2022-06-25) Artica Vicente, Reynaldo Abdias; Ascarza Gallegos, Justo Angelo
Infective endocarditis is one of the major heart conditions that pose a challenge to cardiology, with a high mortality rate. The microorganisms that cause this disease are diverse, with most cases linked to streptococci and staphylococci, and Staphylococcus aureus being the most prevalent in developed countries. In diagnosing infective endocarditis, pathological examination is more sensitive than valve culture. It has been shown that there are cases of culture-negative endocarditis, as well as those negative for conventional Gram staining. However, using the modified Brown-Brenn Gram stain, the result was positive, which led to therapeutic failures due to poor disease management. Considering the importance of the laboratory in aiding diagnosis, this study will evaluate the performance, in terms of sensitivity and specificity, of both the Gram stain and the modified Brown-Brenn Gram stain for diagnosing bacterial endocarditis at the INCOR.
Acceso abierto
Validation of the extract from the Exocarp of Renealmia Alpinia (Kumpia) as a tissue nuclear staining agent
(Universidad Privada Norbert Wiener, 1905-07-06) Santa Cruz Sánchez, Óscar; García Vásquez, Carlos Hugo
From the extract of the exocarp of Renealmia alpinia (kumpia), originating from the Amazon of Peru, through extraction by crushing and subsequent filtration, a 100% pure and natural dye with a purple hue was obtained. This dye underwent various tests of tissue nuclear staining, controlled by time (5, 10, and 15 minutes) and pH measurement, with alcohol at 70° as a mordant. Therefore, the objective of this study was to validate this dye (kumpia) as a tissue nuclear dye. An experimental study was conducted using surgical specimens from the Alberto Sabogal Sologuren Hospital. Tissue samples included liver, intestine, and lymph nodes. A total of 102 slides were obtained (34 for each tissue), of which 51 were stained with kumpia-eosin and 51 with hematoxylin-eosin, taken as the gold standard. Three evaluation criteria were considered in the microscopic assessment: histological image for both kumpia and hematoxylin staining, with results of 88.24% (good) and 11.76% (fair); nuclear staining for kumpia showed 94.12% (good) and 5.88% (fair); and 100% (good) for hematoxylin. For the nuclear-cytoplasm differentiation, both stains showed 100% (good). The conclusion is that this dye is good and effective, offering an easy-to-obtain alternative for tissue nuclear staining, and its cost is very affordable.