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Profile of sensitivity in extended-Spectrum Beta-Lactamase-Producing Enterobacteria isolated from urine cultures of pediatric patients with urinary infections. Hipólito Unanue National Hospital. 2015
(Universidad Privada Norbert Wiener, 1905-07-09) Fonseca Taipe, Francisco; Olivo Lopez, Jose Maria
Urinary tract infections in pediatric patients are mainly caused by enterobacteria, which are gram-negative bacilli capable of producing a variety of beta-lactamase enzymes, including extended-spectrum beta-lactamases, a family of enzymes that inactivate a broader spectrum of beta-lactam antibiotics. OBJECTIVE: To determine the sensitivity profiles of extended-spectrum beta-lactamase-producing enterobacteria isolated from urine cultures of pediatric patients with urinary tract infections at the National Hospital Hipólito Unanue, Lima, 2015. MATERIAL AND METHODS: The data collection tool used was the program Whonet 6.4. A descriptive, quantitative, retrospective, and cross-sectional study was conducted. RESULTS: Of a total of 702 urine cultures analyzed from January to December 2015, 161 strains of gram-negative enterobacteria were isolated. Of these, 40 strains were identified as extended-spectrum beta-lactamase producers (24.8%), using the disk diffusion method on Mueller Hinton agar through the Kirby Bauer technique. To confirm the detection of extended-spectrum beta-lactamases, the Jarlier method was used. The strains mainly identified were E. coli, Klebsiella pneumoniae, and Proteus mirabilis, mostly affecting females (70%) and primarily those aged 1 to 4 years, representing 52.5%. CONCLUSION: The sensitivity profiles of extended-spectrum beta-lactamase-producing enterobacteria to non-beta-lactam antibiotics were: nitrofurantoin (89%), amikacin (87%), gentamicin (47%), showing high resistance to sulfamethoxazole-trimethoprim (95%), nalidixic acid (80%), and ciprofloxacin (70%).