Examinando por Materia "Water Microbiology"
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Publicación Acceso abierto Determinación de la calidad microbiológica e inocuidad del agua potable para consumo de los dispensadores de las boticas y farmacias del distrito de Breña en el departamento de Lima, mayo-junio de 2017(Universidad Privada Norbert Wiener, 2017) Bobadilla Poquioma, Carlos Yoel; Hurtado Cahuana, José Luis; Guevara Vicaña, Rita AuroraWater intended for consumption, the preparation of food and beverages, or personal hygiene must not contain any human pathogens. This can be achieved by selecting high-quality water sources, effectively treating and decontaminating water polluted by human or animal feces or other substances, and protecting it from contamination during distribution to the user. Therefore, it is necessary to determine the presence of microorganisms that indicate water safety (total coliforms) and those that indicate water microbiological quality (Heterotrophs). This was carried out on samples from 67 pharmaceutical establishments in the Breña district during May–June 2017. The determination of coliform microorganisms was performed by the multiple tube method, and the determination of heterotrophic microorganisms was performed by the pour plate count method. The results were compared with the standards established in the Regulation on Drinking Water Quality: D.S. N° 031-2010-SA / Ministry of Health. General Directorate of Environmental Health – Lima: Ministry of Health; 2011. 4.5% of the samples did not comply with the established parameters (Heterotrophs), as the maximum permissible limit according to current national standards is 500 CFU/mL of sample.Publicación Acceso abierto Evaluación de la calidad microbiológica y fisicoquímica del agua para consumo humano de los dispensadores de oficinas farmacéuticas del AA.HH. Mariscal Cáceres del distrito de San Juan de Lurigancho – Lima, 2019(Universidad Privada Norbert Wiener, 2020-08-05) Soto Prudencio, Maria Claudia; Chávez Becerra, María Cleodesvinda; Collanque Pinto, Jesús DanielThe present work entitled “Evaluation of the Microbiological and Physicochemical Quality of Drinking Water from Dispensers in Pharmaceutical Offices of the Human Settlement Mariscal Cáceres, District of San Juan de Lurigancho – Lima, 2019” aimed to evaluate the microbiological and physicochemical quality of drinking water from dispensers. The study was non-experimental, developed in a descriptive and cross-sectional manner, and the analysis consisted of 53 samples. The membrane filtration method was used for the microbiological analysis. The results showed that 15.1% presented values above 500 CFU/mL of heterotrophic bacteria, 24.5% exceeded the maximum permissible limits of total coliforms, 9.4% showed the presence of Escherichia coli, and 28.3% of dispensers were observed in poor condition, of which 20.8% did not meet proper hygiene standards. Meanwhile, 71.7% of dispensers were observed in good condition, but within these, 1.9% did not comply with the recommended hygiene. Regarding physicochemical parameters, two aspects did not meet the permissible limits: appearance and residual free chlorine. In conclusion, 49.1% (n = 26) did not comply, while 50.9% (n = 27) did comply with the microbiological and physicochemical quality of the water. The results were evaluated according to the Regulation on the Quality of Drinking Water for Human Consumption: D.S. No. 031-2010-SA / Directorate General of Environmental Health – Ministry of Health. Lima – Peru, 2011.Publicación Acceso abierto Implementación del método de filtración por membrana empleando el sistema de filtración EZ-FIT™ en análisis microbiológico de agua purificada en laboratorio de caramelos farmacéuticos en el periodo de mayo–octubre del 2019(Universidad Privada Norbert Wiener, 2020-11-27) Minaya Laureano, Maribel Florencia; Guevara Vicaña, Rita AuroraThe objective of this thesis was to compare the implementation of the membrane filtration method using the EZ-FITTM filtration system in a pharmaceutical candy laboratory for the analysis of purified water. The research design was observational, analytical, and descriptive. The study confirmed that the percentage of recovery for the inoculated microorganisms was greater than 70%. It was also evident that the data had a normal distribution, which allowed for the use of the Student's t-test to compare the results of both methods, with a 95% confidence level for the intervals of microbial counts. The comparison was made between the TSA agar medium method and the EZ-FITTM membrane filtration method. This work determined that the mean differences in CFU recovery for E. coli, P. aeruginosa, and S. aureus cultures were not statistically significant, meaning that either technique would provide satisfactory results. Ultimately, according to its established procedures, the laboratory decides which method to apply, prioritizing the benefits of its management.
