“Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor “
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Fecha
2022-11-15Autor(es)
Machado, Suellen
Feitosa, Valker
Pillaca-Pullo, Omar
Lario, Luciana
Sette, Lara
Pessoa, Jr., Adalberto
Alves, Harley
Metadatos
Mostrar el registro completo del ítemResumen
“Microbial proteases, especially aspartic proteases, are an essential group of enzymes
produced from different microorganisms. Microbial proteases have several applications, mainly in
the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing
and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from
the Antarctic environment and was previously reported to have higher extracellular aspartic protease
production. In addition, advances in the operational conditions of bioreactors for enzyme production
are important to reduce the gap associated with scaling−up processes. This is the first study that
evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa
yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose
broth at 25 ◦C for 72 h under kLa values from 18 to 135 h−1
. The results show that kLa (121 h−1
)
obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and
productivity (6.784 U/L.h) as well as biomass (10.4 g/L), µmax (0.14 h−1
) and Yx/s (0.484 g/g). In
conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of
protease expression and secretion influenced by kLa. In this sense, our results could be used for
further industrial investment.“
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- Web of Science (WOS) [236]