Examinando por Autor "Chávez Flores, Juana Elvira"

Mostrando 1 - 20 de 65

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Actividad analgésica del extracto etanólico de la raíz Vallea stipularis L.F. "Chuillur" en ratones
(Universidad Privada Norbert Wiener, 2018-09-18) Picho Hurtado, Katheryn Elizabeth; Chávez Flores, Juana Elvira
The investigation was carried out on the plant species Vallea stipularis L.f. “Chuillur,” located in the district of Tamburco-Abancay-Apurímac, traditionally used by the local population as medicine for the treatment of scurvy, as a cicatrizant, for gastritis, rheumatism, drastic purgative, and analgesic. Objective: To verify the analgesic activity of the ethanolic extract of the root of Vallea stipularis L.f. “Chuillur” in mice. Methods: The method of abdominal writhing induced by 0.8% acetic acid was employed. Forty-nine albino mice of the species Mus musculus strain Balb/C53/CNPB, of both sexes, were randomly distributed into seven groups. Ethanolic extracts at doses of 50, 100, and 200 mg/kg were used, as well as the standards Paracetamol Q.P. 300 mg/kg and Tramadol Hydrochloride Q.P. 40 mg/kg. These were administered orally, and 30 minutes later, 0.8% acetic acid was administered intraperitoneally. The animals were immediately observed for 20 minutes. Results: All groups showed a significant reduction (p value = 0.000), obtaining differentiated analgesic effects. The maximum effect was achieved with the 200 mg/kg extract, with 70% inhibition, surpassing Paracetamol (55%). Conclusions: It is concluded that the ethanolic extract of the root of the plant species Vallea stipularis L.f. “Chuillur” has analgesic effect.
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Actividad analgésica del extracto etanólico de las cáscaras de las pepas Persea Americana Mill "Palta Fuerte" en ratones
(Universidad Privada Norbert Wiener, 2017) Jiménez Colqui, Sofía Gaby; Chávez Flores, Juana Elvira
Persea americana Mill “Strong Avocado” is a plant species that is attributed with medicinal activities, such as antitussive and anti-flu; internal use in respiratory conditions (cold, cough and bronchitis), emmenagogue used in scanty and painful menstruation, Persea americana Mill “Strong Avocado” presents peripheral analgesic activity as well as anti-inflammatory. In the present work, the analgesic activity of the ethanolic extract of the shells of the seeds of Persea americana Mill “Strong Avocado” was verified in albino mice. With the method of Koster et al Modified of writhings induced by 0.6% glacial acetic acid used as a writhing stimulant, the concentrations evaluated were 50, 100 and 200 mg / kg, of extract of the shells of the seeds Persea americana Mill “Strong Avocado”, compared with Tramadol 40 mg / kg and acetylsalicylic acid 200 mg / kg. Obtaining a percentage of reduction in contortions with acetylsalicylic acid 200 mg/kg 88%, Tramadol 40 mg/kg 94%, Persea americana Mill 100 mg/kg 88%, Persea americana Mill 200 mg/kg 94%. The analgesic activity of the ethanolic extract of the shells of the seeds Persea americana Mill at 200 mg/kg was verified and a 94% reduction in contortions compared to Tramadol 40 mg/kg that have the same effect.
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Actividad analgésica y antiinflamatoria del extracto etanólico de la raíz de Taraxacum officinale Wigg “diente león” en ratones (Mus musculus)
(Universidad Privada Norbert Wiener, 2018-05-07) Castañeda Cuadros, Renzo Gerardo; Miranda Velarde, Anthony Boris; Chávez Flores, Juana Elvira
The objective of the study was to determine the analgesic and anti-inflammatory activity of the ethanolic extract from the root of Taraxacum officinale Wigg, "dandelion," in mice. The root was macerated for 7 days in 70% ethanol. Solubility and qualitative analyses were performed, and the extract was determined to be soluble in polar solvents and to contain flavonoids and alkaloids. To determine the analgesic activity, the 0.8% glacial acetic acid-induced abdominal writhing model was used on 49 Balbin/C53/CNPB strain mice. The groups were a negative control (distilled water), a positive control (0.8% acetic acid), the ethanolic extract at 25, 50, and 100 mg/kg, and comparative standards of paracetamol (300 mg/kg) and tramadol (40 mg/kg). To determine the anti-inflammatory effect, the subplantar edema method was used, with dexamethasone (4 mg/kg) and diclofenac (50 mg/kg) as standard drugs. The evaluation of the extract's analgesic activity showed an effective dose of 100 mg/kg with 69% writhing inhibition. The anti-inflammatory activity was effective at a dose of 25 mg/kg, with an inhibition percentage evolving from 56% to 99% over a six-hour period.
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Actividad analgésica y antiinflamatoria del extracto etanólico de las flores de Bidens andicola H.B.K. "quiquo"
(Universidad Privada Norbert Wiener, 2018-03-01) Huarcaya Huarcaya, Liliana; Sotelo Saravia, Nancy Avelina; Chávez Flores, Juana Elvira
The objective of this study was to determine the analgesic and anti-inflammatory activities of the ethanolic extract of Bidens andicola H.B.K “quiquo” flowers. For the analgesic activity, the modified Koster et al. model was employed, and the study groups were: negative control (distilled water), positive control (0.8% glacial acetic acid), ethanolic extract of Bidens andicola H.B.K. “quiquo” flowers at 50, 100, and 200 mg/kg, paracetamol 300 mg/kg, and tramadol 40 mg/kg. For the anti-inflammatory activity, the plantar edema method with 1% albumin was used, and the groups were: negative control (distilled water), ibuprofen 400 mg/kg, dexamethasone 1 mg/kg, and the ethanolic extract at 200, 400, and 600 mg/kg. The results show that the ethanolic extract of Bidens andicola H.B.K. “quiquo” flowers at 100 mg/kg (65%) and 200 mg/kg (70%) exhibits analgesic activity, lower than tramadol (91%) but comparable to paracetamol (81%). Regarding the anti-inflammatory activity, the ethanolic extract at 200, 400, and 600 mg/kg demonstrated efficacy, with the 600 mg/kg dose showing the greatest anti-inflammatory effect (100%), similar to dexamethasone and ibuprofen. It was confirmed that the ethanolic extract of Bidens andicola H.B.K. “quiquo” flowers possesses both analgesic and anti-inflammatory activities.
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Actividad analgésica y antiinflamatoria del extracto hidroalcohólico de las hojas de Passiflora edulis Sims "maracuyá"
(Universidad Privada Norbert Wiener, 2019-03-06) Jalixto Alata, Sonia Carola; Salas Gutierrez, Cristina Angélica; Chávez Flores, Juana Elvira
The species Passiflora edulis Sims, or "maracuyá," is a plant that grows in northern Peru, specifically in the Lima Department, Barranca Province, Supe district, in the Venado Muerto Ravine. This study aimed to evaluate the analgesic and anti-inflammatory activity of the hydroalcoholic extract from the leaves of Passiflora edulis Sims. Methodology: A total of 56 Holtzmann strain rats of different sexes were used for the evaluation of inflammation. The rats were divided into 8 groups of seven animals. Inflammation was induced using the 0.1 mL albumin-induced plantar edema method, and the plantar volume was measured with a plethysmometer every hour for 6 hours. For the analgesic activity evaluation, the 0.8% acetic acid-induced abdominal writhing method was employed. A total of 49 Mus musculus strain mice of different sexes were divided into 7 groups of seven animals. The number of abdominal writhes was individually quantified over a 20-minute period. Results and Conclusion: The results demonstrated anti-inflammatory activity, as the 800 mg/kg hydroalcoholic extract inhibited the development of plantar edema with an effect of 100% within one hour of application, comparable to 4 mg of dexamethasone. The study also showed the analgesic activity of the 400 mg/kg hydroalcoholic extract, which achieved an effect of 70%.
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Actividad Analgésica y Antiinflamatoria del Extracto Hidroalcohólico de las hojas de Ruellia graecizans Backer (Paque-paque) en ratones.
(Universidad Privada Norbert Wiener, 2017-11-27) Becerra Mejía, Einsten Román; Chávez Flores, Juana Elvira
El presente trabajo de investigación tuvo como objetivo evaluar la Actividad Analgésica y Antiinflamatoria del extracto hidroalcohólico de las hojas de Ruellia graecizans Backer (Paque-paque) en ratones. La recolección de las hojas de la especie vegetal se realizó en el centro poblado el Rollo, distrito de Choros, provincia de Cutervo, departamento de Cajamarca, a una altitud de 1900 m.s.n.m. Se realizó la prueba de solubilidad y análisis cualitativo. Se utilizaron 84 ratones albinos con un peso corporal superior a 25 g de ambos sexos (cepa Balbin/C53/CNPB). Para determinar la actividad analgésica se utilizó el modelo de contorsiones abdominales por ácido acético glacial al 0,8 % (Koster y Col. Modificado), se utilizó el extracto de la especie vegetal en concentraciones de 50, 100 y 200 mg/kg y como estándar Paracetamol Q.P. 300 mg/kg y Tramadol 40 mg/kg, obteniendo como resultado que el extracto hidroalcohólico de las hojas de Ruellia graecizans Backer (Paque-paque) presentan un mejor efecto analgésico a una concentración de 50 mg/kg con un porcentaje de inhibición de contorsiones de 81 % siendo comparable con el Paracetamol Q.P. 300 mg/kg. Para determinar la actividad antiinflamatoria se empleó el método del edema subplantar inducido por albumina 1 % (Winter et al. Modificado), se utilizó el extracto de la especie vegetal en concentraciones de 50, 100 y 150 mg/kg y como estándar Dexametasona 4 mg/kg y Diclofenaco 50 mg/kg, obteniendo como resultado que el extracto hidroalcohólico de las hojas de Ruellia graecizans Backer (Paque-paque) a las 6 horas de estudio presenta un mayor efecto antiinflamatorio a una dosis de 50 mg/kg con un porcentaje de inflamación de 0,75 %.
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Actividad analgésica y antiinflamatoria del extracto hidroalcólico de hojas y tallos de Leucheria daucifolia (D. Don.) Crisci "Churoq wasin" en ratones
(Universidad Privada Norbert Wiener, 2018-12-07) Soto Requez, Yvon Carla; Ruiz Rondinel, Sandra; Chávez Flores, Juana Elvira
The present study aimed to evaluate the analgesic and anti-inflammatory activity of the hydroalcoholic extract of leaves and stems of Leucheria daucifolia (D. Don.) Crisci “Churoq wasin”. For the analgesic activity, the model of abdominal writhing induced by 0.8% acetic acid was employed; 49 mice were used, and the evaluated groups were: negative control (distilled water), positive control (0.8% acetic acid), hydroalcoholic extract at 50, 100 and 200 mg/kg, compared with paracetamol 300 mg/kg and tramadol 40 mg/kg. For the anti-inflammatory effect, the auricular edema method was used with an irritant agent (6% xylene diluted in acetone) applied to the mouse auricle. A total of 49 albino mice were used, and the study groups were: vaseline cream, creams prepared with the hydroalcoholic extract at 0.5, 1 and 2%, compared with diclofenac 1% cream and hydrocortisone 1% cream. The results show that the hydroalcoholic extract of leaves and stems of Leucheria daucifolia (D. Don.) Crisci “Churoq wasin” at a dose of 100 mg/kg presents analgesic activity with an inhibition percentage of writhing of 62%, not surpassing tramadol (86%) but comparable to paracetamol (76%). Regarding anti-inflammatory activity, the cream with 2% extract presented an inhibition percentage of inflammation of 48.7%, which is comparable to hydrocortisone 1% and diclofenac 1%. It was confirmed that the hydroalcoholic extract possesses both analgesic and anti-inflammatory activity in mice.
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Actividad anti-tirosinasa y efecto fotoprotector del extracto etanólico del tegumento de dos variedades de Phaseolus vulgaris L., ñuña roja y negra
(Universidad Privada Norbert Wiener, 2020-12-01) Mera Santa Cruz, Ermitanio; Papuico Sanchez, Liliana Eulalia; Chávez Flores, Juana Elvira
The objective of this study was to determine the anti-tyrosinase activity and evaluate the photoprotective effect of the ethanolic extract from the tegument of two varieties of Phaseolus vulgaris L., red and black 'ñuña'. Methodology: Anti-tyrosinase activity was determined with the thin-layer chromatographic method using a 0.05% Kojic acid standard. For the photoprotective effect, 35 female Mus musculus Balb/C53 strain mice, seven weeks old, were divided into five experimental groups. UV-C radiation was applied to their backs for 30 minutes once a day for one month. Acute photodamage was evaluated using the Draize scale, and photoaging was evaluated using the Glogau scale. Results: Secondary metabolites such as polyphenols and flavonoids were found. The extract was not toxic in mice via oral and dermal administration at a limit dose of 5000 mg/kg. It has anti-tyrosinase activity, and when applied in a 5% gel, the red variety showed a greater effect. It also has a photoprotective effect in mice subjected to UVC radiation. Conclusion: The ethanolic extract from the tegument of the black 'ñuña' at 5% in gel has a photoprotective effect.
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Actividad antiinflamatoria del extracto etanólico de las hojas del Baccharis buxifolia (Lam.) Pers. “Talla” en ratones
(Universidad Privada Norbert Wiener, 2018-08-22) Rivera Vicuña, David Walter; Chávez Flores, Juana Elvira
The present research aimed to evaluate the anti-inflammatory activity of the ethanolic extract of Baccharis buxifolia (Lam.) Pers. “Talla” leaves in mice. Solubility testing and a qualitative phytochemical profile of the ethanolic extract were carried out. To determine anti-inflammatory activity, the subplantar edema method with 1% albumin, as described by Winter et al. (modified), was employed. Forty-two albino mice (Mus musculus strain Balb/C53) of both sexes, each weighing more than 25 g, were used. The mice were randomly distributed into six groups and subsequently administered 0.1 mL of 1% albumin in the subplantar region. The plant extract was tested at concentrations of 100, 200, and 400 mg/kg of body weight, while dexamethasone (4 mg/kg) and diclofenac (50 mg/kg) were used as standard drugs. The results showed that the ethanolic extract of Baccharis buxifolia leaves, at 400 mg/kg, exhibited the greatest anti-inflammatory effect at two hours, with an inhibition percentage of 62%, compared with the lower extract concentrations (100 mg/kg: 10%; 200 mg/kg: 28%) and with the standard drugs diclofenac (50 mg/kg: 31%) and dexamethasone (4 mg/kg: 48%). It is concluded that the ethanolic extract of Baccharis buxifolia (Lam.) Pers. “Talla” leaves demonstrates significant anti-inflammatory activity at a dose of 400 mg/kg.
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Actividad antimicrobiana del extracto etanólico de las hojas de Schinus molle L. "molle"
(Universidad Privada Norbert Wiener, 2017) Clemente Sotteccani, Claudia Elizabeth; Paucar Lopez, Rosmery; Chávez Flores, Juana Elvira
Peru is one of the countries with the greatest biodiversity in the world, and many of its plant species can be sustainably utilized by industry. Schinus molle L. ("Molle"), a native tree with medicinal applications whose ethanolic extract exhibits antibacterial properties, is a clear example. The objective of this research was to confirm the antimicrobial activity of the ethanolic extract from the leaves of Schinus molle L. To this end, the ethanolic extract was obtained at concentrations of 500 and 1000 mg/mL, with 0.12% chlorhexidine gluconate as the positive control and distilled water as the negative control. The study of antimicrobial activity was conducted using the disk diffusion method with surface plating technique. The experimental solutions were incubated under anaerobic conditions for 48 and 72 hours at 37∘ C, after which the diameters of the inhibition halos were read with a vernier caliper. A qualitative analysis determined the presence of metabolites: flavonoids, alkaloids, carbohydrates, steroids and/or triterpenes, reducing sugars, and phenolic compounds. Results: The ethanolic extract at concentrations of 500 and 1000 mg/mL demonstrated antimicrobial activity against Streptococcus mutans ATCC 25175. From the comparison performed, chlorhexidine gluconate produced greater inhibition. Conclusion: The ethanolic extract of Schinus molle L. 'Molle' presents antimicrobial activity against Streptococcus mutans ATCC 25175, and the chlorhexidine gluconate is qualitatively similar to the ethanolic extract of Schinus molle L. 'Molle'.
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Actividad antioxidante y antiinflamatoria del extracto etanólico de las hojas de Passiflora tripartita var. mollissima (Kunth) "tumbo serrano"
(Universidad Privada Norbert Wiener, 2019-03-08) Ramos Huamaní, Hilda; Pari Colque, Regina; Chávez Flores, Juana Elvira
The plant species Passiflora tripartita var. mollissima (Kunth), commonly known as “tumbo serrano,” is native to Peru and grows in the regions of Cuzco, Lambayeque, Lima, Pasco, Piura, and Talara. The objective of this study was to determine the antioxidant and anti-inflammatory activity of the ethanolic extract of Passiflora tripartita var. mollissima leaves. The ethanolic extract was obtained by maceration, after which solubility testing and phytochemical screening were conducted to identify secondary metabolites. Antioxidant activity was evaluated using the DPPH method, while anti-inflammatory activity was assessed through the paw edema test induced by a phlogistic agent (1% albumin). The results demonstrated antioxidant activity at the following concentrations: 125 µg/mL (79.55%), 93.75 µg/mL (60.99%), 62.5 µg/mL (41.93%), and 31.25 µg/mL (21.82%). Compared with the TROLOX standard, the extract also showed significant anti-inflammatory activity, with a dose of 600 mg/kg producing an effect from the first hour of administration (p = 0.000), which was comparable to that of dexamethasone at 4 mg. In conclusion, the ethanolic extract of Passiflora tripartita var. mollissima leaves exhibits both antioxidant and anti-inflammatory activity.
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Actividad antiulcerosa del extracto etanólico de las hojas de Ficus carica L. "Higo" en ratas
(Universidad Privada Norbert Wiener, 2018-09-17) Abarca Vega, Erika Victoria; Chávez Flores, Juana Elvira
Objective: To determine the antiulcer activity of the ethanolic extract of Ficus carica L. (“Fig”) leaves in rats. An ethanolic maceration of the plant leaves was carried out, followed by solubility testing, preliminary phytochemical analysis, and evaluation of antiulcer activity using the modified Lee (1971) technique at doses of 300, 600, and 800 mg/kg. A total of 60 Holtzman rats were randomly distributed into six groups, induced with gastric ulcer using naproxen 200 mg/kg, with ranitidine 30 mg/kg as the reference drug. The extract was shown to be soluble in methanol, ethanol, and distilled water. The preliminary phytochemical screening revealed the presence of flavonoids, alkaloids, phenolic compounds, and free amino groups. Regarding the antiulcer activity, the most effective treatment was the Ficus carica L. leaf extract at 800 mg/kg, achieving 83.01% inhibition of gastric ulceration, compared with ranitidine, which achieved 73.58% inhibition. These results were obtained using the Marhuenda scale. It was determined that the ethanolic extract of Ficus carica L. leaves at a dose of 800 mg/kg presents antiulcer activity in rats.
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Actividad cicatrizante del extracto hidroalcohólico de las hojas de Ruellia graecizans Backer (paque-paque) en ratones albinos Mus Musculus CEPA BALB/C53
(Universidad Privada Norbert Wiener, 2020-08-13) Heredia Luis, Nuria Lissbeth; Chávez Flores, Juana Elvira
The objective of this research was to confirm the wound-healing activity of the hydroalcoholic extract from the leaves of Ruellia graecizans Backer, 'paque-paque,' in Balb/C53 strain albino mice (Mus musculus). Methodology: The plant was collected in El Rollo, Cutervo, Cajamarca, at an altitude of 1900 m.a.s.l. Solubility tests and a qualitative analysis were performed on the hydroalcoholic extract. A total of 70 mice with an average body weight of 30-40 g were distributed into seven groups. To determine wound-healing activity, gels with the extract at various concentrations (1%, 2%, 5%, and 10%) were prepared and applied to induced lesions using the method described by Vaisberg et al. (1989). These gels were compared to a standard gel and a control base gel. The results showed that the 10% gel had a healing efficacy of over 80%, comparable to the standard gel. Conclusion: The wound-healing activity of the 10% gel based on the topical application of the Ruellia graecizans Backer 'paque-paque' leaf extract was confirmed.
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Actividad cicatrizante del extracto hidroalcohólico de los tallos de Tripogandra serrulata (M. Vahl) Handlos “7 vidas” en ratones albinos
(Universidad Privada Norbert Wiener, 2015) Machuca Cóndor, John José; Chávez Flores, Juana Elvira
This experimental study determined the wound healing effect of the hydroalcoholic extract of Tripogandra serrulata (M. Vahl) Handlos (“7 vidas”) stems in mice. The plant material was collected in the district of Pangoa, province of Satipo, department of Junín, at 628 m a.s.l. The stems were macerated to obtain a homogeneous plant sample. A phytochemical analysis was performed, and topical creams were prepared with different concentrations of the hydroalcoholic extract (5%, 10%, 15%, and 20%), which were used for pharmacological testing according to the method described by Vaisberg et al. (1989), in induced lesions. A total of 56 albino mice (Mus musculus Balb/C53), of both sexes, were distributed into seven groups. The treatment with the greatest efficacy among the prepared topical creams was the 15% concentration. It was concluded that the hydroalcoholic extract of Tripogandra serrulata (M. Vahl) Handlos (“7 vidas”) stems exhibits wound healing activity.
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Actividad cicatrizante y toxicidad dérmica del extracto etanólico de los tubérculos de Ullucus tuberosus Caldas “olluco” en animales de experimentación
(Universidad Privada Norbert Wiener, 2018-05-28) Blacido Paucar, Zayda Judith; Quispe Crisóstomo, Nicols Hilario; Chávez Flores, Juana Elvira
The use of medicinal plants as an alternative to treat ailments and/or diseases is gaining great importance for the creation and research of new formulations. Objective: To determine the wound-healing activity and dermal toxicity of the ethanolic extract from the tubers of Ullucus tuberosus Caldas, 'olluco,' in experimental animals. Methods: The plant species was collected in the Panao community, Huánuco department, Peru. An ethanolic extraction of the Ullucus tuberosus Caldas 'olluco' tuber was performed for phytochemical analysis. The wound-healing activity was assessed using the Vaisberg et al. technique, for which three creams based on the plant species were prepared at concentrations of 0.5,5, and 10%. These were compared with two standard groups: a 1% Croton Lechleri 'Sangre de Drago' cream prepared naturally, and onion plant stem cells (Emolan®), and a control group ('Vaseline'). Dermal toxicity was determined using the Contero technique, where a single oral and dermal dose of 5000 mg/kg of the extract was administered to rats. Results: Phytochemical analysis determined that the extract is soluble in distilled water and contains primary and secondary metabolites. The 10% concentration cream obtained 86% healing efficacy compared to the intact skin sample. In the toxicological evaluation, no Holtzman strain rat showed dermal toxicity. Conclusion: It was determined that the ethanolic extract from the tubers of Ullucus tuberosus Caldas, 'olluco,' does present wound-healing activity and does not possess dermal toxicity.
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Actividad diurética del extracto hidroalcohólico de las hojas secas de Baccharis genistelloides (Lam.) pers. (Carqueja) en ratas albinas de la cepa Holtzman
(Universidad Privada Norbert Wiener, 2021-05-20) López Dávila, Zaid Hebert; Chávez Flores, Juana Elvira
Medicinal plants with diuretic activity currently occupy a place as an alternative therapy to pharmaceutical drugs. Objective: To determine the diuretic activity of the hydroalcoholic extract from the dry leaves of Baccharis genistelloides (Lam.) Pers. (Carqueja) in Holtzman strain albino rats. Materials and method: 50 female rats weighing 230 to 280 g were used, employing the modified Naik et al. model. The rats were pre-hydrated with 0.9% sodium chloride (NaCl) at 50 mL/kg and distributed into five groups: Control Group (0.9% NaCl 50 mL/kg), Standard Group (furosemide 40 mg/kg), Group I (extract 200 mg/kg), Group II (extract 400 mg/kg), and Group III (extract 600 mg/kg). Urine was collected after 6 hours, followed by electrolyte dosage and the calculation of the percentage of urinary volumetric excretion (%EVU) and the percentage of diuretic activity (%AD). Results: At the sixth hour, the highest average urine volume was observed in Group III (8.63 mL). This group also showed 55.7% EVU. Regarding %AD, Group III presented the highest activity at 113.1%, which was superior to the control group. For electrolyte dosage, the observed differences in K+ and Na+ were not significant, meaning it cannot be asserted that the extract significantly alters these electrolytes, while the Cl- values of the extract groups were comparable to the control group. Conclusions: The hydroalcoholic extract from the dry leaves of Baccharis genistelloides (Lam.) Pers. (Carqueja) presented diuretic activity at all three concentrations used.
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Actividad hipoglicemiante y evaluación de la capacidad del extracto etanólico de la semilla de Persea americana Mill "palto" para reducir el daño histológico en células β pancreáticas
(Universidad Privada Norbert Wiener, 2019-06-17) García Chávez, Yelson Néstor; Ortega Ponce, Dany Lizbeth; Chávez Flores, Juana Elvira
The objectives were to determine the hypoglycemic activity and evaluate the capacity of the ethanolic extract from the seed of Persea americana Mill, 'Palto' (avocado), to reduce histological damage in pancreatic β cells. Methodology: The ethanolic extract was obtained by collecting, chopping, and drying the seed. Maceration was performed for 7 days. The Szkudelski method was used, which induces diabetes with alloxan, and the hypoglycemic evaluation was performed using test strips. After the study concluded, the animals were sacrificed, and histological sections of the pancreas were prepared. Results: The seed extract showed solubility in water. Treatment with the 200 mg/kg dose was the most effective in reducing glucose. The histological sections showed that the damage produced by alloxan was minor in the pancreatic β cells of the mice treated with the extract of the species under study. Conclusion: The ethanolic extract of the seed was successfully obtained and the solubility test was performed. The extract applied in mice presents hypoglycemic activity via the Szkudelski method, and it was determined that the seed extract has the capacity to reduce histological damage in pancreatic β cells.
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Actividad laxante del extracto etanólico de las hojas de Origanum majorana L. “mejorana” en ratones
(Universidad Privada Norbert Wiener, 2018-12-04) Cáceres Milla, Alexander Jesús; Chávez Flores, Juana Elvira
The objective of this study was to determine the laxative activity of the ethanolic extract of Origanum majorana L. (“marjoram”) in mice. The species was collected in the department of Ayacucho, macerated for seven days, and concentrated using an extraction hood and a Memmert oven at 40 °C until a dry sample was obtained. To analyze intestinal motility, the method described by Vogel et al. was employed, applying the direct observation technique. A total of 48 albino mice of the Balb/C53/CNPB strain, both male and female, were used, with a dose of 300 mg/kg. The extract demonstrated greater laxative activity. In conclusion, the ethanolic extract of Origanum majorana L. (“marjoram”) exhibits laxative activity when administered orally.
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Actividad repelente in vitro de los aceites esenciales de Syzygium aromaticum "clavo de olor" y Cinnamomum verum "canela" sobre Aedes Aegypti
(Universidad Privada Norbert Wiener, 2022-05-13) Abanto Rojas, Rita Norma; Alvarez Torres, Eugenia Albertina; Chávez Flores, Juana Elvira
The objective of this study was to evaluate the in vitro repellent activity of the essential oils of Syzygium aromaticum ('clove') and Cinnamomum verum ('cinnamon') and a 50/50 v/v combination of both oils on Aedes aegypti. The essential oils were obtained by steam distillation, and the area preference method was used to evaluate repellent activity. The repellent activity of the essential oils and the 50/50 v/v combination was evaluated on the Aedes aegypti Rockefeller strain at various concentrations (0.2%, 0.1%, 0.05%, 0.03%, and 0.01%). N,N-diethyl-3-methylbenzamide (DEET) at 15% was used as a positive control, and acetone was used as a negative control. It was concluded that the essential oils of Syzygium aromaticum, Cinnamomum verum, and their combination do have in vitro repellent activity on Aedes aegypti. The minimum repellent concentration was 0.03%, and the repellency time was five (5) hours at concentrations of 0.05%, 0.1%, and 0.2%. Furthermore, the in vitro repellency level was found to be between class IV and V (using the Talukder and Howes rating) at the 0.05%, 0.1%, and 0.2% concentrations from the 1st to the 5th hour.
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Actividad sedante y ansiolítico del extracto etanólico de las hojas de Ruta chalepensis L. “ruda” en ratones
(Universidad Privada Norbert Wiener, 2018-06-05) Alvarado Lopez, Lesly Lizeth; Castro Bolaños, Cinthia Melissa; Chávez Flores, Juana Elvira
Ruta chalepensis L., "Ruda," is a plant widely distributed in the Mediterranean region and used in folk medicine as an antispasmodic, anthelmintic, antimicrobial, emmenagogue, and abortifacient. The plant species was collected in the Lima department, Lurín district, at 1224 m.a.s.l.. In this experimental study, the sedative and anxiolytic effect of the ethanolic extract from the leaves of Ruta chalepensis L., "Ruda," was confirmed in mice. A qualitative phytochemical profile was conducted. Subsequently, the study methods began: Sodium Pentobarbital-induced Sleep at different concentrations (100,250, and 500 mg/kg) and the Marble Burying Test at doses of 5,10, and 15 mg/kg. A total of 100 albino Balb/C53 strain mice of both sexes were used and distributed into five groups. It was confirmed that the most effective treatment in the Marble Burying Test was the ethanolic extract of Ruta chalepensis L., 'Ruda,' at 10 and 15 mg/kg, showing an effect superior to diazepam. Regarding the Sodium Pentobarbital-induced Sleep method, the comparison of the extracts versus diazepam indicates that the ethanolic extracts of Ruda at 250 and 500 mg/kg primarily have an effect similar to diazepam. Conclusion: The ethanolic extract from the leaves of Ruta chalepensis L., "Ruda," does possess sedative and anxiolytic activity.