Examinando por Autor "Puchoc Quispe, Sayuri Yasumi"

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Acceso abierto
Determinación de etanol en sangre en individuos examinados por dos métodos analíticos en el laboratorio central de la Dirección de Criminalística de la Policía Nacional del Perú. Lima 2023
(Universidad Privada Norbert Wiener, 2024-02-27) Puchoc Quispe, Sayuri Yasumi; Vega Salazar, Antony; Ramos Jaco, Antonio Guillermo
The objective of this study was to validate the methods for blood alcohol analysis using GC-FID and UV-VIS and to determine the differences between ethanol concentrations in blood samples from individuals tested at the Central Laboratory of DIRCRI-PNP. The methodology employed was the hypothetical-inductive method, with a quantitative approach, basic and descriptive, longitudinal, and of comparative level. The performance characteristics evaluated in the validation were: selectivity, sensitivity, linearity, accuracy, precision, and robustness, using blood samples with ethanol. For the determination of ethanol in individuals, 240 biological blood samples from the Toxicology Service – DIRCRI-PNP Sampling were processed and analyzed using the GC-FID and UV-VIS methods. Data processing for validation was performed using ANOVA, linear regression, F-test, and Student's t-test applied to the mentioned methods. The performance characteristics of the GC-FID and UV-VIS methods were: detection limits of 0.00191 g/L and 0.0123 g/L; quantification limits of 0.00636 g/L and 0.0411 g/L; accuracy as % recovery of 96.59–100.16% and 86.17–101.16%; repeatability as coefficient of variation of 2.01% and 26.47%; intermediate precision as coefficient of variation of 1.88% and 22.11%. Conclusion: The GC-FID method cannot be interchangeable or replaceable by UV-VIS due to the high variability in the performance characteristics of the methods.