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URI permanente para esta comunidadhttps://hdl.handle.net/20.500.13053/384
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Examinando Doctorado por Materia "Aceites Volátiles"
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Publicación Acceso abierto Efecto inhibidor de las concentraciones mínimas del aceite esencial de muña y orégano en comparación con sustancias antimicrobianas usadas en la terapia pulpar frente a cepas de enterococcus faecalis. Estudio in vitro. Lima 2014(Universidad Privada Norbert Wiener, 2017-02-28) Malpartida Quispe, Federico Martin; Aguirre Morales, Anita KoriThe aim of this study was to determine the inhibitory effect of minimum concentrations of essential oil of Minthostachys mollis (muña) and Origanum vulgare (orégano) comparing them with antimicrobial substances used in pulp therapy against strains of Enterococcus faecalis. For this purpose, the E. faecalis (ATCC 29212) strains were reactivated and used in the two stages of the study.1. Determination of the minimum inhibitory concentration (MIC) of both essential oils. 2. Determination of the inhibitory effect of these essential oils compared to antimicrobial substances used in pulp therapy. In the first stage, it was found that the CMM of muña essential oil was 10% and the CMM of the oregano essential oil was 30%. In the second stage, E. faecalis were seeded in 56 petri dishes containing Mueller Hinton culture medium with wells of 6mms. of diameter filled with 100 ml of 100% and 10% (CMI) muña essential oil,30% and 100% (CMI) of oregano essential oil, sodium hypochlorite al 5%, chlorhexidine gel at 2% and chlorhexidine solution al 2%, Calcifar - P ® y Tween 20. The petris were incubated at 37 ° C performing the measurement of the inhibition halos with a Vernier caliper or caliper rule at 24 and 48 hours. For the statistics analysis ANOVA and Tukey tests were used. We concluded that the CMM of muña and oregano essential oils have inhibitory effect against E. faecalis at 24 and 48 hours, however its effect is less than 2% chlorhexidine (solution).
