Examinando por Materia "Alkaloids"

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Acceso abierto
Actividad analgésica y antiinflamatoria del extracto hidroalcohólico de las hojas de Passiflora edulis Sims "maracuyá"
(Universidad Privada Norbert Wiener, 2019-03-06) Jalixto Alata, Sonia Carola; Salas Gutierrez, Cristina Angélica; Chávez Flores, Juana Elvira
The species Passiflora edulis Sims, or "maracuyá," is a plant that grows in northern Peru, specifically in the Lima Department, Barranca Province, Supe district, in the Venado Muerto Ravine. This study aimed to evaluate the analgesic and anti-inflammatory activity of the hydroalcoholic extract from the leaves of Passiflora edulis Sims. Methodology: A total of 56 Holtzmann strain rats of different sexes were used for the evaluation of inflammation. The rats were divided into 8 groups of seven animals. Inflammation was induced using the 0.1 mL albumin-induced plantar edema method, and the plantar volume was measured with a plethysmometer every hour for 6 hours. For the analgesic activity evaluation, the 0.8% acetic acid-induced abdominal writhing method was employed. A total of 49 Mus musculus strain mice of different sexes were divided into 7 groups of seven animals. The number of abdominal writhes was individually quantified over a 20-minute period. Results and Conclusion: The results demonstrated anti-inflammatory activity, as the 800 mg/kg hydroalcoholic extract inhibited the development of plantar edema with an effect of 100% within one hour of application, comparable to 4 mg of dexamethasone. The study also showed the analgesic activity of the 400 mg/kg hydroalcoholic extract, which achieved an effect of 70%.
Acceso abierto
Actividad cicatrizante del extracto hidroalcohólico de las hojas de Ruellia graecizans Backer (paque-paque) en ratones albinos Mus Musculus CEPA BALB/C53
(Universidad Privada Norbert Wiener, 2020-08-13) Heredia Luis, Nuria Lissbeth; Chávez Flores, Juana Elvira
El objetivo de esta investigación fue: Comprobar la Actividad cicatrizante del extracto hidroalcohólico de las hojas de Ruellia graecizans Backer “paque-paque” en ratones albinos Mus musculus cepa Balb/C53. Metodología: La especie vegetal fue colectada en el centro poblado el Rollo, distrito de Choros, provincia de Cutervo, departamento de Cajamarca, con una altitud de 1900 m.s.n.m. Con el extracto hidroalcohólico de las hojas “paque-paque”, se realizó pruebas de solubilidad y análisis cualitativo. Se utilizaron 70 ratones, con un peso corporal promedio de 30 -40 g de ambos sexos (cepa Balb/C53/CNPB), los cuales fueron distribuidos en siete grupos. Para determinar la actividad cicatrizante, se preparó geles a base del extracto hidroalcohólico de las hojas “paque-paque” en distintas concentraciones (1, 2, 5 y 10%), que fueron aplicados a los ratones, utilizando el método descrito por Vaisberg y Col. (1989), en lesiones inducidas; estos geles fueron comparados con el grupo estándar (extracto líquido de cebolla 10 g, heparina sódica 0.04 g (5000 U.I), alantoína 1 g, excipientes c.s.p 100 g. ) gel ®, y el grupo control gel Base, se obtuvo de resultado, que el extracto hidroalcohólico de las hojas “paque-paque” tiene actividad cicatrizante con el gel al 10% con una eficacia de cicatrización superior al 80%, siendo comparable con el (extracto líquido de cebolla 10 g, heparina sódica 0.04 g (5000 U.I), alantoína 1 g, excipientes c.s.p 100 g. ) gel®. En conclusión, se comprobó la actividad cicatrizante del gel al 10% a base del extracto de hojas Ruellia graecizans Backer “paque-paque” por vía tópica.
Acceso abierto
Estudio fitoquímico y evaluación de la toxicidad aguda del extracto hidroalcohólico de las hojas frescas de Tanacetum vulgare L., "Palma real"
(Universidad Privada Norbert Wiener, 2013) Chávez Gonzales, Luz Marina; Gutiérrez Condori, Darwin Armando; Chávez Flores, Juana Elvira
A phytochemical and toxicological study of the hydroalcoholic extract of fresh leaves of Tanacetum vulgare L. (“Palma real”) was carried out. The species was collected in the district of Tamburco, Province of Abancay, Department of Apurímac. A hydroalcoholic extraction of the fresh leaves was performed. Through solubility tests and phytochemical analysis, it was determined that the extract is soluble in polar solvents and contains primary metabolites such as carbohydrates and free amino groups, as well as secondary metabolites including alkaloids, steroids, flavonoids, and phenolic compounds. Alkaloids were extracted using an alkaline medium; a fraction (F1) was identified by thin-layer chromatography, employing a MeOH-CHCl₃ solvent system (1:1.5 v/v), and subsequently isolated by preparative chromatography. A possible chemical structure was identified through ultraviolet-visible and infrared spectroscopic analyses, revealing amino, carbonyl, and aromatic groups. For the toxicological study, the Betancourt method was applied, using an acute toxicity model with a limit dose of 2000 mg/kg in Mus musculus Balb/C-53 mice. Histopathological sections of perfused organs showed damage to the liver and kidney. Very mild toxicity was observed in the stomach, while the lung preserved its histopathological structure.