Examinando por Materia "Alkaloids"

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Acceso abierto
Actividad analgésica y antiinflamatoria del extracto hidroalcohólico de las hojas de Passiflora edulis Sims "maracuyá"
(Universidad Privada Norbert Wiener, 2019-03-06) Jalixto Alata, Sonia Carola; Salas Gutierrez, Cristina Angélica; Chávez Flores, Juana Elvira
The species Passiflora edulis Sims, or "maracuyá," is a plant that grows in northern Peru, specifically in the Lima Department, Barranca Province, Supe district, in the Venado Muerto Ravine. This study aimed to evaluate the analgesic and anti-inflammatory activity of the hydroalcoholic extract from the leaves of Passiflora edulis Sims. Methodology: A total of 56 Holtzmann strain rats of different sexes were used for the evaluation of inflammation. The rats were divided into 8 groups of seven animals. Inflammation was induced using the 0.1 mL albumin-induced plantar edema method, and the plantar volume was measured with a plethysmometer every hour for 6 hours. For the analgesic activity evaluation, the 0.8% acetic acid-induced abdominal writhing method was employed. A total of 49 Mus musculus strain mice of different sexes were divided into 7 groups of seven animals. The number of abdominal writhes was individually quantified over a 20-minute period. Results and Conclusion: The results demonstrated anti-inflammatory activity, as the 800 mg/kg hydroalcoholic extract inhibited the development of plantar edema with an effect of 100% within one hour of application, comparable to 4 mg of dexamethasone. The study also showed the analgesic activity of the 400 mg/kg hydroalcoholic extract, which achieved an effect of 70%.
Acceso abierto
Actividad cicatrizante del extracto hidroalcohólico de las hojas de Ruellia graecizans Backer (paque-paque) en ratones albinos Mus Musculus CEPA BALB/C53
(Universidad Privada Norbert Wiener, 2020-08-13) Heredia Luis, Nuria Lissbeth; Chávez Flores, Juana Elvira
The objective of this research was to confirm the wound-healing activity of the hydroalcoholic extract from the leaves of Ruellia graecizans Backer, 'paque-paque,' in Balb/C53 strain albino mice (Mus musculus). Methodology: The plant was collected in El Rollo, Cutervo, Cajamarca, at an altitude of 1900 m.a.s.l. Solubility tests and a qualitative analysis were performed on the hydroalcoholic extract. A total of 70 mice with an average body weight of 30-40 g were distributed into seven groups. To determine wound-healing activity, gels with the extract at various concentrations (1%, 2%, 5%, and 10%) were prepared and applied to induced lesions using the method described by Vaisberg et al. (1989). These gels were compared to a standard gel and a control base gel. The results showed that the 10% gel had a healing efficacy of over 80%, comparable to the standard gel. Conclusion: The wound-healing activity of the 10% gel based on the topical application of the Ruellia graecizans Backer 'paque-paque' leaf extract was confirmed.
Acceso abierto
Estudio fitoquímico y evaluación de la toxicidad aguda del extracto hidroalcohólico de las hojas frescas de Tanacetum vulgare L., "Palma real"
(Universidad Privada Norbert Wiener, 2013) Chávez Gonzales, Luz Marina; Gutiérrez Condori, Darwin Armando; Chávez Flores, Juana Elvira
A phytochemical and toxicological study of the hydroalcoholic extract of fresh leaves of Tanacetum vulgare L. (“Palma real”) was carried out. The species was collected in the district of Tamburco, Province of Abancay, Department of Apurímac. A hydroalcoholic extraction of the fresh leaves was performed. Through solubility tests and phytochemical analysis, it was determined that the extract is soluble in polar solvents and contains primary metabolites such as carbohydrates and free amino groups, as well as secondary metabolites including alkaloids, steroids, flavonoids, and phenolic compounds. Alkaloids were extracted using an alkaline medium; a fraction (F1) was identified by thin-layer chromatography, employing a MeOH-CHCl₃ solvent system (1:1.5 v/v), and subsequently isolated by preparative chromatography. A possible chemical structure was identified through ultraviolet-visible and infrared spectroscopic analyses, revealing amino, carbonyl, and aromatic groups. For the toxicological study, the Betancourt method was applied, using an acute toxicity model with a limit dose of 2000 mg/kg in Mus musculus Balb/C-53 mice. Histopathological sections of perfused organs showed damage to the liver and kidney. Very mild toxicity was observed in the stomach, while the lung preserved its histopathological structure.