Examinando por Materia "Bacterial inhibition"

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(Universidad Privada Norbert Wiener, 2015) Carrión Reyes, Gueraldin Mercedes; Malpartida Quispe, Federico Martin
The objective of this study was to determine the inhibitory effect of the ethanolic extract of Bidens pilosa (Amor seco) compared to Colgate Plax® and 0.12% Chlorhexidine Gluconate against strains of Streptococcus mutans. The study was experimental, prospective, longitudinal, and analytical. The population consisted of Streptococcus mutans strains (ATCC 25175), and the sample consisted of 40 Petri dishes. The strains were reactivated and cultured on Blood Agar, where 6 mm diameter wells were made, and approximately 100 µl of the ethanolic extract of Bidens pilosa (Amor seco) were added in the following concentrations: 0.8/10, 1/10, and pure. The results were compared with 0.12% Chlorhexidine Gluconate as a positive control, distilled water as a negative control, and Colgate Plax® mouthwash. The results showed that the 0.8/10 and 1/10 concentrations of the ethanolic extract of Bidens pilosa (Amor seco) did not present any inhibition zone at any time. However, the pure ethanolic extract produced an average inhibition zone of 11.98 mm at 24 hours and 11.80 mm at 48 hours. Furthermore, a stronger inhibitory effect was observed with the positive control, 0.12% Chlorhexidine Gluconate, and a lesser inhibitory effect compared to Colgate Plax® mouthwash against the Streptococcus mutans (ATCC 25175) strain.
Acceso abierto

(Universidad Privada Norbert Wiener, 2015) Carrión Reyes, Fiorella Isamar; Malpartida Quispe, Federico Martin
The objective of this study was to determine the inhibitory effect of the essential oil of Schinus molle (Molle) compared to 0.12% Chlorhexidine Gluconate and Colgate Plax® mouthwash in inhibiting bacterial growth of Streptococcus mutans in vitro. The study was experimental, cross-sectional, prospective, and analytical at an explanatory level, conducted in a microbiology laboratory. The population consisted of Streptococcus mutans strains (ATCC 25175), with a sample of 40 Petri dishes. The agar diffusion method was used with 6 mm diameter wells, where 100 µl of Schinus molle (Molle) essential oil at 25%, 50%, and 100%, 0.12% Chlorhexidine Gluconate, Colgate Plax® mouthwash, and Tween 20 were poured. The results showed that the concentrations of 50% and 100% Schinus molle essential oil produced an average inhibition zone of 10.97 mm and 10.46 mm at 24 hours, and 11.06 mm and 10.59 mm at 48 hours, which were smaller than the inhibition zones formed by 0.12% Chlorhexidine Gluconate and Colgate Plax® mouthwash at both times against the Streptococcus mutans (ATCC 25175) strain.
Acceso abierto

(Universidad Privada Norbert Wiener, 2017-09-29) Chávez Hidalgo, Diego Andrés; Cupé Araujo, Ana Cecilia
Materials and Methods: Ten toothpaste brands available in the local market were used, with pediatric toothpastes recommended by specialists in pediatric dentistry. Streptococcus mutans (ATCC 25175) was cultured on a specific medium (tryptic soy agar, yeast extract, sucrose, and bacitracin), which is ideal for the recovery and evaluation of Streptococcus mutans, as referenced in studies certifying its suitability. Bacitracin was used to allow only the growth of this bacterium, preventing contamination in our cultures. The agar diffusion method was used, where the agar is inoculated with the bacterial strain to ensure uniform growth. A modification was made to the preparation of the agar, adding 50 ml of sterile lamb blood to create contrast between the agar and inhibition halos. The cylinder-plate technique from the United States Pharmacopeia was employed, which involves using calibrated cylinders (made of surgical steel or porcelain) measuring 10 mm in height and having an external diameter of 8 mm and an internal diameter of 6 mm. These cylinders were placed on the plates with a spacing of 6 cylinders per plate, following the plate's perimeter and ensuring distance between them for the formation of inhibition halos. According to the technique's protocol, 6 Petri dishes were used for each toothpaste evaluated, 6 plates for evaluating the Gold Standard (penicillin plus gentamicin), and another 6 Petri dishes for the negative evaluation. Conclusions: The toothpaste brands sold in the local market showed inhibitory activity against Streptococcus mutans (ATCC 25175), except for one.
Acceso abierto
Antibacterial effect of Stevia rebaudiana, Camellia sinensis and Origanum vulgare against Streptococcus mutans in vitro study, Lima - 2023
(Universidad Privada Norbert Wiener, 2024-06-04) Mamani Turpo, Luz Delia; Chilón Minaya, Lesly Johanna
The aim of this study was to determine the antibacterial effect of three plant-based compounds: Stevia rebaudiana, Camellia sinensis, and Origanum vulgare, against Streptococcus mutans. An in vitro experimental design methodology was used, with an analytical method and applied type. Treatments were applied to Petri dishes with Streptococcus mutans strains, using 10 plates for each treatment and two control groups. The results revealed that, of the three plant-based compounds tested, two showed antibacterial effects against Streptococcus mutans. For Origanum vulgare, inhibition halos of 32.09 mm were obtained at 24 hours and 31.80 mm at 48 hours. For Camellia sinensis, inhibition halos were 25.06 mm at 24 hours and 24.74 mm at 48 hours. However, Stevia rebaudiana did not show an antibacterial effect as its inhibition halos were 0 mm. Therefore, it was concluded that the antibacterial compounds that did show antibacterial effects did not show significant differences compared to the positive control used, 0.12% Chlorhexidine Gluconate (p>0.05).
Acceso abierto
Bacterial inhibitory effect of essential oil of Eucalyptus Globulus against standardized strain of Enterococcus Faecalis and Phorphyromonas Gingivalis in vitro study, Lima - 2023
(Universidad Privada Norbert Wiener, 2024-08-05) Girón Guayanay, Laura Paola; Velasquez Velasquez, Roxana Pilar
This research aimed to determine the antibacterial inhibitory effect of Eucalyptus globulus essential oil against standardized strains of Enterococcus faecalis and Porphyromonas gingivalis in an in vitro study, Lima - 2023. A quantitative methodology with an analytical focus was used, combining practical application and a prospective quasi-experimental design of a longitudinal nature. A sample size of 160 agar plates was employed, distributed into two main groups: 80 plates for the evaluation of Enterococcus faecalis strains and 80 for the evaluation of Porphyromonas gingivalis. These were further subdivided into four subgroups of 20 Petri plates each. Each subgroup was assigned a specific treatment, which included 20 plates with Eucalyptus globulus essential oil compounds at 50%, 20 with compounds at 75%, 20 with compounds at 100%, and 20 plates with physiological serum as a control. A specific evaluation form was used for the study, where the data generated during the laboratory activities were recorded. The results concluded that there were significant differences in the antibacterial inhibitory effect of Eucalyptus globulus essential oil against standardized strains of Enterococcus faecalis and Porphyromonas gingivalis (p <0.001).